Phylogenomic and divergence time data for 81 vespid species and four apid outgroups: Concatenated matrices, partitions, phylogenetic trees, and chronograms
Data files
Sep 14, 2026 version files 282.80 MB
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README.md
10.71 KB
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Supplementary.zip
282.79 MB
Abstract
This dataset comprises phylogenomic analysis files for 85 species of Hymenoptera, including 81 species of Vespidae and four outgroup species of Apidae. The data include amino acid sequence matrices (USCOs), ultraconserved element (UCE) datasets, partition files, phylogenetic trees, and time-calibrated trees used for phylogenomic and divergence time analyses. Single-copy nuclear orthologs (USCOs) were extracted from genome and transcriptome assemblies, and UCEs were extracted from genome assemblies. All files are provided in standard formats (.fasta, .nexus, .phy, .tre) to facilitate reuse in comparative phylogenomic, evolutionary, and taxonomic studies of aculeate Hymenoptera. The dataset is associated with a manuscript on the phylogenomics and divergence time estimation of Vespidae. No sensitive data are included; the data are released under CC0 to maximize reuse potential.
Supplementary
We generated new genomic data for 32 vespid species (Palearctic and Oriental regions) and combined these with publicly available data from 53 species (NCBI), totaling 85 species. The dataset includes UCEs and USCOs for phylogenetic and divergence time analyses.
Author(s)
Zheng Wei
Chongqing Key Laboratory of Control and Utilization of Vector Insects, Institute of Entomology and Molecular Biology, College of Life Science, Chongqing Normal University, Chongqing 401331, China; 2024110513010@stu.cqnu.edu.cn
Dai Min
Chongqing Key Laboratory of Control and Utilization of Vector Insects, Institute of Entomology and Molecular Biology, College of Life Science, Chongqing Normal University, Chongqing 401331, China; 2021110513042@stu.cqnu.edu.cn
Carpenter James M
Division of Invertebrate Zoology, American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA; carpente@amnh.org
Luo Li
Chongqing Key Laboratory of Control and Utilization of Vector Insects, Institute of Entomology and Molecular Biology, College of Life Science, Chongqing Normal University, Chongqing 401331, China; lioly1994@163.com
Chen Bin
Chongqing Key Laboratory of Control and Utilization of Vector Insects, Institute of Entomology and Molecular Biology, College of Life Science, Chongqing Normal University, Chongqing 401331, China; bin.chen@cqnu.edu.cn
He Shulin
Chongqing Key Laboratory of Control and Utilization of Vector Insects, Institute of Entomology and Molecular Biology, College of Life Science, Chongqing Normal University, Chongqing 401331, China; shulin-he@cqnu.edu.cn
Li Tingjing
Chongqing Key Laboratory of Control and Utilization of Vector Insects, Institute of Entomology and Molecular Biology, College of Life Science, Chongqing Normal University, Chongqing 401331, China; ltjing1979@cqnu.edu.cn
File list (files found within Supplementary.zip)
- FigTree_excluding_all_node.tre
- FigTree_excluding_node1.tre
- FigTree_excluding_node2.tre
- FigTree_excluding_node3.tre
- FigTree_excluding_node4.tre
- FigTree_excluding_node5.tre
- FigTree_excluding_node6.tre
- Figtree_run1.tre
- Figtree_run2.tre
- Species names corresponding to UCEs.txt
- Species names corresponding to USCOs.txt
- uce50_astral_gscf.cf.tree
- uce50_FcC_info.csv
- uce50_FcC_supermatrix.phy
- uce50_ghost_gscf.cf.tree
- uce50_partition_gscf.cf.tree
- uce90_astral_gscf.cf.tree
- uce90_FcC_info.csv
- uce90_FcC_supermatrix.phy
- uce90_ghost_gscf.cf.tree
- uce90_partition_gscf.cf.tree
- uce100_astral_gscf.cf.tree
- uce100_FcC_info.csv
- uce100_FcC_supermatrix.phy
- uce100_ghost_gscf.cf.tree
- uce100_partition_gscf.cf.tree
- usco50 astral_gscf.cf.tree
- usco50_fasttree_gscf.cf.tree
- usco50_FcC_info.csv
- usco50 FcC_supermatrix.phy
- usco50_ghost_gscf.cf.tree
- usco50_pmsf_gscf.cf.tree
- usco80_astral_gscf.cf.tree
- usco80_fasttree_gscf.cf.tree
- usco80_FcC_info.csv
- usco80_FcC_supermatrix.phy
- usco80_ghost_gscf.cf.tree
- usco80_pmsf_gscf.cf.tree
- usco90_astral_gscf.cf.tree
- usco90_fasttree_gscf.cf.tree
- usco90_FcC_info.csv
- usco90_FcC_supermatrix.phy
- usco90_ghost_gscf.cf.tree
- usco90_partition_gscf.cf.tree
- usco90_pmsf_gscf.cf.tree
File descriptions
- FigTree_excluding_all_node.tre -MCMCTree chronogram obtained from the sensitivity analysis excluding all calibration points except the root(i.e., retaining only the root calibration).
- FigTree_excluding_node1.tre -MCMCTree chronogram from the leaveoneout sensitivity analysis excluding calibration node 1 (see Table S? for node definitions).
- FigTree_excluding_node2.tre -MCMCTree chronogram from the leaveoneout sensitivity analysis excluding calibration node 2.
- FigTree_excluding_node3.tre -MCMCTree chronogram from the leaveoneout sensitivity analysis excluding calibration node 3.
- FigTree_excluding_node4.tre -MCMCTree chronogram from the leaveoneout sensitivity analysis excluding calibration node 4.
- FigTree_excluding_node5.tre -MCMCTree chronogram from the leaveoneout sensitivity analysis excluding calibration node 5.
- FigTree_excluding_node6.tre -MCMCTree chronogram from the leaveoneout sensitivity analysis excluding calibration node 6.
- Figtree_run1.tre -MCMCTree chronogram from the first independent MCMC run (100 million generations, sampled every 100 generations, 20 % burnin).
- Figtree_run2.tre -MCMCTree chronogram from the second independent MCMC run (same settings as run 1), used to assess convergence.
- Species names corresponding to UCEs.txt -Lookup table mapping species codes to full scientific names for all taxa included in the UCE datasets.
- Species names corresponding to USCOs.txt -Lookup table mapping species codes to full scientific names for all taxa included in the USCO datasets.
- uce50_astral_gscf.cf.tree -Phylogeny of Vespidae inferred from matrix UCE 50 % using the MSC model implemented in ASTRAL, with BPP/gCF/sCF.
- uce50_FcC_info.csv -Summary statistics of the concatenated UCE 50 % supermatrix generated by FASconCAT. Includes overall matrix statistics (total sites, percentage of missing data, indels, ambiguities, parsimonyinformative sites, GC content) and perlocus details, with start and end positions for each UCE locus in the supermatrix, allowing visualization of the partition scheme.
- uce50_FcC_supermatrix.phy -Concatenated supermatrix (PHYLIP format) for the UCE 50%_fna dataset used for phylogenetic tree inference.
- uce50_ghost_gscf.cf.tree -Maximum likelihood species tree for UCE 50 % inferred under the GHOST model (GTR+FO*H4) in IQTREE, with SH-aLRT/UFBoot/gCF/sCF.
- uce50_partition_gscf.cf.tree -Maximum likelihood species tree for UCE 50 % inferred under the partitioned model (GTR+I+G) in IQTREE, with SH-aLRT/UFBoot/gCF/sCF.
- uce90_astral_gscf.cf.tree -Phylogeny of Vespidae inferred from matrix UCE 90 % using the MSC model implemented in ASTRAL, with BPP/gCF/sCF.
- uce90_FcC_info.csv -Summary statistics of the concatenated UCE 90 % supermatrix generated by FASconCAT, including overall matrix statistics and perlocus details with partition boundaries.
- uce90_FcC_supermatrix.phy -Concatenated supermatrix (PHYLIP format) for the UCE 90%_fna dataset used for phylogenetic tree inference.
- uce90_ghost_gscf.cf.tree -Maximum likelihood species tree for UCE 90 % inferred under the GHOST model (GTR+FO*H4) in IQTREE, with SH-aLRT/UFBoot/gCF/sCF.
- uce90_partition_gscf.cf.tree -Maximum likelihood species tree for UCE 90 % inferred under the partitioned model (GTR+I+G) in IQTREE, with SH-aLRT/UFBoot/gCF/sCF.
- uce100_astral_gscf.cf.tree -Phylogeny of Vespidae inferred from matrix UCE 100 % using the MSC model implemented in ASTRAL, with BPP/gCF/sCF.
- uce100_FcC_info.csv -Summary statistics of the concatenated UCE 100 % supermatrix generated by FASconCAT, including overall matrix statistics and perlocus details with partition boundaries.
- uce100_FcC_supermatrix.phy -Concatenated supermatrix (PHYLIP format) for the UCE 100%_fna dataset used for phylogenetic tree inference.
- uce100_ghost_gscf.cf.tree -Maximum likelihood species tree for UCE 100 % inferred under the GHOST model (GTR+FO*H4) in IQTREE, with SH-aLRT/UFBoot/gCF/sCF.
- uce100_partition_gscf.cf.tree -Maximum likelihood species tree for UCE 100 % inferred under the partitioned model (GTR+I+G) in IQTREE, with SH-aLRT/UFBoot/gCF/sCF.
- usco50_astral_gscf.cf.tree -Phylogeny of Vespidae inferred from matrix USCO 50 % using the MSC model implemented in ASTRAL, with BPP/gCF/sCF.
- usco50_fasttree_gscf.cf.tree -ML species tree for USCO 50 % inferred using FastTree (approximate ML) with the LG+CAT substitution model, with gCF and sCF values, with UFBoot2/gCF/sCF.
- usco50_FcC_info.csv -Summary statistics of the concatenated USCO 50 % supermatrix generated by FASconCAT, including overall matrix statistics and perlocus details with partition boundaries.
- usco50_FcC_supermatrix.phy -Concatenated supermatrix (PHYLIP format) for the USCO 50%_faa dataset used for phylogenetic tree inference.
- usco50_ghost_gscf.cf.tree -Maximum likelihood species tree for USCO 50 % inferred under the GHOST model (LG+FO*H4) in IQTREE, with SH-aLRT/UFBoot/gCF/sCF.
- usco50_pmsf_gscf.cf.tree -ML species tree for USCO 50 % inferred under the PMSF model, with SH-aLRT/UFBoot2/gCF/sCF.
- usco80_astral_gscf.cf.tree -Phylogeny of Vespidae inferred from matrix USCO 80 % using the MSC model implemented in ASTRAL, with BPP/gCF/sCF.
- usco80_fasttree_gscf.cf.tree -ML species tree for USCO 80 % inferred using FastTree (approximate ML) with the LG+CAT substitution model, with UFBoot2/gCF/sCF.
- usco80_FcC_info.csv -Summary statistics of the concatenated USCO 80 % supermatrix generated by FASconCAT, including overall matrix statistics and perlocus details with partition boundaries.
- usco80_FcC_supermatrix.phy -Concatenated supermatrix (PHYLIP format) for the USCO 80%_faa dataset used for phylogenetic tree inference.
- usco80_ghost_gscf.cf.tree -Maximum likelihood species tree for USCO 80 % inferred under the GHOST model (LG+FO*H4) in IQTREE, with SH-aLRT/UFBoot/gCF/sCF.
- usco80_pmsf_gscf.cf.tree -ML species tree for USCO 80 % inferred under the PMSF model, with SH-aLRT/UFBoot2/gCF/sCF.
- usco90_astral_gscf.cf.tree -Phylogeny of Vespidae inferred from matrix USCO 90 % using the MSC model implemented in ASTRAL, with BPP/gCF/sCF.
- usco90_fasttree_gscf.cf.tree -ML species tree for USCO 90 % inferred using FastTree (approximate ML) with the LG+CAT substitution model, with UFBoot2/gCF/sCF.
- usco90_FcC_info.csv -Summary statistics of the concatenated USCO 90 % supermatrix generated by FASconCAT, including overall matrix statistics and perlocus details with partition boundaries.
- usco90_FcC_supermatrix.phy -Concatenated supermatrix (PHYLIP format) for the USCO 90%_faa dataset used for phylogenetic tree inference.
- usco90_ghost_gscf.cf.tree -Maximum likelihood species tree for USCO 90 % inferred under the GHOST model (LG+FO*H4) in IQTREE, with SH-aLRT/UFBoot/gCF/sCF.
- usco90_partition_gscf.cf.tree -Maximum likelihood species tree for USCO 90 % inferred under the partitioned model (LG+I+G) in IQTREE, with SH-aLRT/UFBoot/gCF/sCF.
- usco90_pmsf_gscf.cf.tree -ML species tree for USCO 90 % inferred under the PMSF model, with SH-aLRT/UFBoot2/gCF/sCF.
