Dataset DOI: 10.5061/dryad.pc866t24k

Description of the data and file structure

This dataset contains physiological and proteomic measurements from a controlled laboratory experiment designed to investigate how food availability, thermal history, and sirtuin activity influence the response of the California mussel (Mytilus californianus) to aerial heat stress. Mussels were acclimated for three weeks to four combinations of food ration (low or high) and daily emersion temperature (20 or 30 °C) in tidal-simulation tanks that reproduced natural circadian and tidal cycles. Following acclimation, mussels were exposed to an acute aerial heat stress (33 °C), either with or without prior exposure to the sirtuin inhibitors nicotinamide and suramin, and allowed to recover under their respective acclimation conditions. The dataset includes label-free proteomic analyses of gill tissue, measurements of ciliary activity and particle transport velocity, clearance rates, valve gape and siphon activity, and associated experimental metadata. 

Files and variables

File: DisciplineSpecificMetadata.json

Description: Overview of LC-MS parameters used in study. More information can be found at PRIDE database: https://www.ebi.ac.uk/pride/archive/projects/PXD072074

File: All_Fc.csv

Description: Fold change data for proteomics calculated in MSstats. 

Variables

• Accession: NCBI accession number
• NCBI Name: Protein name attached to the NCBI accession number
• Uniprot.Accession: Uniprot accession number 
• Protein.Name: Protein name attached to the Uniprot accession number
• Label: Statistical comparison for fold change of time x treatment x acclimation groups, where BL = Baseline XP1 or 2 = experimental day 1 or 2; HTHF = high food high temperature, LTLF = low temperature low food, HTLF = high temperature low food, LTHF = low temperature high food; Inh - sirtuin inhibition treatment before heat shock; HS = heat shock only treatment 
• log2FC: Fold change on Log2 scale
• SE: standard error for fold change
• Tvalue: T statistic from Student T test
• DF: Degrees of Freedom
• pvalue: raw p value from model
• adj.pvalue: adjusted p value for multiple comparisons
• issue: Issues with model
• Comparison: which statistical comparisons were made. Inh - within time and acclimation group, compared Inh to HS; BL - effect of treatment group to baseline measurements (no inhibition); XP = comparing Inh groups from Day 2 and 1. 
• Acclimation: Acclimation group, where HTHF = high food high temperature, LTLF = low temperature low food, HTLF = high temperature low food, LTHF = low temperature high food
• MissingPercentage: proportion of missing intensity measurements
• Significant: Whether the fold change was significant for a = 0.05 (Yes or No)

File: TideSimDDRampMaster.zip

Description: Arduino code for controlling tidal simulator tanks.

File: ClearanceRateData.csv

Description: Data for clearance rates measurements published at https://doi.org/10.1016/j.jembe.2020.151510 

Variables

• Before or After: Before (Baseline) or After treatment (heat shock or sirtuin and heat shock)
• Time: Arbitrary time point for organizing data
• Hours to Heat Shock: Actual time to or following heat shock in hours
• Tide: Night (N) or Day (D) time high tide
• Acclimation Tank: Acclimation group, where HTHF = high food high temperature, LTLF = low temperature low food, HTLF = high temperature low food, LTHF = low temperature high food
• Impact: Experimental treatment group
• Mussel: label of individual animal, marked with a bee dot (corresponding to color and number)
• Log CR + 1: Log +1 of clearance rate measurements in L h-1

File: SiphonOpeningData.csv

Description: Data for sihpon opening measurements published at https://doi.org/10.1016/j.jembe.2020.151510 

Variables

• Treatment: Experimental group, where BL = baseline, HS = heat shock only, and HS-I is heat shock following sirtuin inhibition
• TimeofDay: Day (D) or Night (N)
• Time to HS: Time before or after heat shock treatment in hours
• Day: Day of experiment within treatment
• Time: Arbitrary time for sorting data
• Replicate: Technical replicate - videos recorded in increments
• Food: High (H) or Low (L) food group
• Temperature: High (H) or Low (L) temperature group
• Inhibition: Whether received sirtuin inhibition, Y = yes, N = no
• Basket: Arbitrary assignment for acclimation
• Beaker: Beaker number the animal was placed in for measurement
• Mussel: label of individual animal, marked with a bee dot (corresponding to color and number)
• Wet Weight: mass of animal recorded after experiment was conducted. 
• PercentOpen: Percent of time the mussel spent with the valves open
• Scaled %/g: Siphon activity (percent time open) scaled to mussel size (wet weight)

File: TideSimFeedingDDRamp.zip

Description: Arduino code for controlling feeding pulses into tidal simulator tanks.

Code/software

Arduino was used to program the tidal simulator tanks. All other data provided in csv. Information for proteomics analysis linked in PRIDE Project PXD072074.

Access information

Other publicly accessible locations of the data:

• https://www.ebi.ac.uk/pride/archive/projects/PXD072074

Data was derived from the following sources:

• N/A