Two species of the Entoloma quadratum–murrayi complex from Japan, E. kermesinum sp. nov. and E. flavescens sp. nov.
Data files
Nov 22, 2024 version files 408.25 KB
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ITS.fasta
35.29 KB
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ML_with_BS_ITS.nwk
2.15 KB
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ML_with_BS_mtLSU.nwk
585 B
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ML_with_BS_single-copy-gene.nwk
2.02 KB
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mtLSU.fasta
2.38 KB
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README.md
1.68 KB
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single_copy_gene.fasta
361.94 KB
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whole_AIC_partitioned_codonpartitioned.partition
2.22 KB
Abstract
We describe two new species, Entoloma. kermesinum sp. nov. and E. flavescens sp. nov., which are confused with E. quadratum and E. murrayi, respectively. We sequenced the large subunit of mitochondrial ribosomal RNA, the nuclear ribosomal internal transcribed spacer region and 22 single-copy genes for 51 specimens of E. kermesinum, E. flavescens, E. album, and related species. Species boundaries were assessed using the molecular phylogenetics and population genetics approaches. Specimens of E. kermesinum, E. flavescens, and E. album formed independent clades, which were phylogenetically distinct from the specimens of E. quadratum and E. murrayi collected around the type locality (i.e., New England). Although the phylogenetic distance between E. flavescens and E. album was small, gene flow between them was restricted in areas where they coexisted, suggesting reproductive isolation. Therefore, these five species can be treated as independent species. We found characteristics useful for identifying E. kermesinum and E. flavescens. In particular, E. kermesinum is characterized by a crimson to brown-red and fibrillose pileus, finely covered by whitish fibrous scales; E. flavescens is characterized by a lemon-yellow to tan and shiny-to-silky pileus. In addition, relatively large basidiospores and clamp connections are diagnostic features of these two species.
Input and output data of phylogenetic analyses in “Two new species of the Entoloma quadratum-murrayi complex from Japan, E. kermesinum sp. nov. and E. flavescens sp. nov.
DATA-SPECIFIC INFORMATION FOR INPUT FILES of MOLECULAR PHYLOGENETIC INFERENCE
[single_copy_gene.fasta]
A multiple sequence alignment file (fasta format) of 21 single copy genes used for reconstructing ML tree using RAxML. Alignment data contain concatenated sequences of 21 single copy genes (7,432 bp).
[whole_AIC_partitioned.partition]
A partition file for thelignment file (“single_copy_gene.fasta”) used for reconstructing ML tree using RAxML
[MLtree_with_BS_single-copy-gene.nwk]
ML tree with bootstrap values reconstructed from concatnated sequences of 21 single copy genes using RAxML (newick format)
[ITS.fasta]
A multiple sequence alignment file (fasta format) of the nuclear ribosomal internal transcribed spacer (ITS) region used for reconstructing ML tree using RAxML. Alignment data contain concatenated sequences of ITS1 and ITS2 regions (727 bp).
[MLtree_with_BS_ITS.nwk]
ML tree with bootstrap values reconstructed from concatnated sequences of ITS1 and ITS2 regions using RAxML (newick format)
[mtLSU.fasta]
A multiple sequence alignment file (fasta format) of the large subunit of mitochondrial ribosomal RNA (mtLSU) used for reconstructing ML tree using RAxML. Alignment data contain concatenated sequences of mtLSU (282 bp).
[MLtree_with_BS_mtLSU.nwk]
ML tree with bootstrap values reconstructed from mtLSU sequences using RAxML (newick format)