Data from: Dual oxidase is essential for molting, hatching and feeding in the brown planthopper
Data files
Apr 24, 2025 version files 21.72 KB
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5th_instar_H2O2_measurement.csv
796 B
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Body_length_and_weight.csv
1.56 KB
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EPG_and_salivary_sheath.csv
1.81 KB
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Ovarian_expression.csv
773 B
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README.md
3.07 KB
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Reproduction_related_data.csv
1.07 KB
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RNAi_efficiency.csv
1.53 KB
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Survival_rate.csv
2.83 KB
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Temporal_expression.csv
4.72 KB
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Tissue_specific_expression.csv
1.19 KB
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VC_treatment_and_H2O2_rescue.csv
2.39 KB
Abstract
Dual oxidase (Duox) is well-known for its role in immunity and tyrosine crosslinking activity across various biological processes from mammals to holometabolous insects. Nevertheless, its function in hemimetabolous insects remains poorly understood. In this study, we explored the physiological roles of the Duox gene in a hemimetabolous insect, the brown planthopper. We conducted series of experiments, this dataset is the experimental data derived from our manuscript, including qRT-PCR data, survival rate data, hydrogen peroxide content data, body weight and length data, feeding behavior related data, and reproduction related data.
Dataset DOI: 10.5061/dryad.q2bvq83ww
Description of the data and file structure
We conducted series of experiments，this dataset is the experimental data derived from our manuscript, including qRT-PCR data (Temporal/Tissue-specific/Ovarian/RNAi efficiency), survival rate data, hydrogen peroxide content data, body weight and length data, feeding behavior related data, Vc treatment and hydrogen peroxide rescue data, and reproduction related data.
Files and variables
File: 5th_instar_H2O2_measurement.csv
Description: Hydrogen peroxide level was measured 84 h after dsRNA-injection or at 0/48/84 h 5th-instar nymphs.
Variables
- H2O2 level (μmol/g)
File: Body_length_and_weight.csv
Description: Body length and weight of females were measured.
Variables
- length(mm)
- weight(mg)
File: EPG_and_salivary_sheath.csv
Description: The EPG results are divided into four stages, and the data shown are the time proportions of each stage. Number of salivary sheaths from day1 to day3 were calculated.
Variables
- EPG: NP/PP/N4/N5
- Salivary sheathes: d1/d2/d3
File: Ovarian_expression.csv
Description: qRT-PCR result of Duox gene expression in the ovary.
Variables
- Day2-Day5
File: Reproduction_related_data.csv
Description: Eggs number and hatchability of 15 females after dsRNA-injection.
Variables
- Number of eggs
- Hatchability(%)
File: RNAi_efficiency.csv
Description: The RNAi efficiency was detected through qRT-PCR experiments.
Variables
- RNAi efficiency of 3rd-5th instar nymphs
- RNAi efficiency of egg
- RNAi efficiency of ovary
File: Survival_rate.csv
Description: The survival rates of 3rd-5th instar nymphs after RNAi.
Variables
- 3rd-5th
File: Temporal_expression.csv
Description: The temporal expression was detected through qRT-PCR experiments.
Variables
- egg/1st-5th instar/Adult female/Adult male
File: Tissue_specific_expression.csv
Description: The tissue-specific expression was detected through qRT-PCR experiments.
Variables
- Integument (Int), fat body (FB), gut (G), ovary (O), salivary gland (SG), and brain (B), and testis (T)
File: VC_treatment_and_H2O2_rescue.csv
Description:
Survival rates of vitamin C-feeding nymphs. Newly molted 5th-instar nymphs were fed an artificial diet supplemented with 25–100 mg/mL of vitamin C. H2O2 levels in vitamin C-fed nymphs were measured.
Newly molted 5th-instar nymphs were injected with dsRNA and then injected with 20 nmol H2O2 or PBS 12 h before eclosion.
Variables
VC-treatment
- Control
- 25mg/mL
- 50mg/mL
- 100mg/mL
H2O2-injection
- dsGFP+PBS
- dsDuox+H2O2（20nmol）
- dsDuox+PBS
Code/software
All data are saved in csv files and can be viewed through excel or other software capable of viewing csv files.
For qRT-PCR data, relative transcript levels were calculated using the 2−ΔΔCt method. All data were expressed as mean ± SD, based on at least three replicates. Statistical analyses were performed using GraphPad Prism 8.0 (GraphPad Software, CA, USA). Comparisons between two treatments were conducted using Student’s t test or Welch’s t test. Multiple comparisons were analyzed using one-way ANOVA with Games-Howell post hoc tests for non-homogeneous variances. Statistical significance was set at p < 0.05 (*) and p < 0.01 (**).