Biological data for ATSB-IVM/blood assays with Aedes aegypti

Silva, Alexandre 1 ; Moura, Thais1; Dias, Alyne1

Published Mar 17, 2026 on Dryad. https://doi.org/10.5061/dryad.q573n5txd

Data files

Mar 17, 2026 version files 630.21 KB

Data_Moura_et_al_MVE.prism

626.23 KB
README.md

3.98 KB

Abstract

This data repository contains the raw and analyzed results supporting the evaluation of an Attractive Toxic Sugar Bait (ATSB) formulated with ivermectin (IVM) against Aedes aegypti females.

The dataset includes quantitative results used to determine the impact of ATSB-IVM ingestion (15 ppm) on critical biological and reproductive parameters of colonized Aedes aegypti. Data were collected from females aged 5–7 days allocated to two main treatment groups: ingestion of the sugar bait before blood feeding (BB) and ingestion after blood feeding (AB).

The repository includes a primary data table in Prism format detailing the following metrics across three time points (48, 72, and 96 hours relative to blood feeding or bait ingestion):

Blood Engorgement Rate: Percentage of females that successfully took a blood meal.
Female Survival: Measured over the experimental period.
Reproductive Traits: Including Oviposition Rate, total Egg Number (Fecundity), and Egg Hatching Rate (Fertility).

The raw data supports the reported findings that ATSB-IVM ingestion causes pronounced sublethal effects, with significantly greater negative impacts observed in the BB group and in the AB group at the longest exposure time point (96 h). These data are available for re-analysis and validation of ATSB-IVM efficacy as a mosquito control strategy.

Dataset DOI: 10.5061/dryad.q573n5txd

Description of the data and file structure

The data from the study titled "Ingestion of Attractive Toxic Sugar Baits (ATSB) Containing Ivermectin Before and After Blood Feeding Affects the Biology and Reproduction of Aedes aegypti (Diptera: Culicidae)," submitted to the journal Medical and Veterinary Entomology, were collected during experiments conducted at the Insect Bioecology Laboratory of the Federal University of Rondônia during the undergraduate research project of the first author. We used sugar baits containing ivermectin, which were offered to mosquitoes before or after a blood meal at different intervals between 48 and 96 hours. The objective was to evaluate several variables: 1. blood engorgement after bait offering or bait engorgement after blood feeding (Blood/Bait engorgement); 2. survival until oviposition (Survival); 3. oviposition rate (Oviposition); 4. number of eggs laid (Eggs); and 5. larvae ecloded (Eclosion). Variables 1-5 were observed during the first and second gonotrophic cycles of the mosquito to evaluate the residual effect of the treatment.

Files and variables

File: Data_Moura_et_al_MVE.prism

Description: The data in the file were placed in grouped data spreadsheets, with columns indicating the type of bait, i.e., ASB (Attractive Sugar Bait - sugar control) and ATSB (Attractive Toxic Sugar Bait - containing ivermectin - used as an insecticide). Rows indicate the time until blood feeding after bait offering, i.e., 48, 72, or 96 hours, with the group designated as "Baits Before Blood Feeding" (BB), or the time until bait offering after blood feeding, i.e., 48, 72, or 96 hours, with the group designated as "Baits After Blood Feeding" (AB). Each variable was collected during the first gonotrophic cycle (1 cycle) and the second gonotrophic cycle (2 cycle).

A few examples for data sheet description in the Prism file:

Blood engorgement 1 cycle-BB - Blood engorgement - number of mosquitoes that ingested blood divided by the inicial number of mosquitoes that ingested bait denoted by BB (Bait before Blood Feeding) during the first gonotrophic cycle denoted by 1 cycle.

Bait engorgement 1 cycle - AB - Bait engorgement - number of mosquitoes that ingested bait divided by the inicial number of mosquitoes that ingested blood denoted by AB (bait After Blood feeding) during the first gonotrophic cycle denoted by 1 cycle.

Survival 1 cycle - BB - Survival - number of mosquitoes that survived until oviposition divided by the number of mosquitoes that ingested blood after the ingestion of the bait denoted by BB (Bait before Blood Feeding) during the first gonotrophic cycle denoted by 1 cycle.

Oviposition 2 cycle -BB - Oviposition - number of mosquitoes that laid eggs divided by the inicial number of mosquitoes that survived until oviposition that ingested blood after the ingestion of the bait denoted by BB (Bait before Blood feeding) during the second gonotrophic cycle denoted by 2 cycle.

Eggs 1 cycle - AB - Eggs - number of eggs laid by mosquitoes that ingested bait after blood feeding denoted by AB (bait After Blood feeding) during the first gonotrophic cycle denoted by 1 cycle.

Eclosion 1 cycle - BB - Eclosion - number of larvae that ecloded from eggs laid by mosquitoes that ingested bait before blood feeding denoted by BB (Bait before Blood feeding) during the first gonotrophic cycle denoted by 1 cycle.

Code/software

The data can be viewed using the free visualization version of the Prism statistical software (GraphPad), available at the following link: https://www.graphpad.com/support/faq/where-can-i-find-the-free-prism-viewer/