Here we present HAUpekinT2T, a complete, telomere-to-telomere (T2T) gapless reference genome assembly for the Pekin duck (Anas platyrhynchos). To overcome the challenges of highly repetitive regions and complex genomic structures, this high-quality genome was assembled using a combination of long-read sequencing (Pacbio HiFi and Nanopore) and Hi-C chromatin conformation capture technologies. The final assembly spans approximately 1.26 Gb with a contig N50 of 79.69 Mb. It successfully achieves gapless assemblies for 41chromosomes, including the complete resolution of complex regions such as centromeres, telomeres, and the sex chromosomes (Z and W).