Dataset DOI: 10.5061/dryad.qz612jmx4

Description of the data and file structure

Principle Investigator Contact Information

Name: Peixuan Guo
Institution: The Ohio State University
Email: guo.1091@osu.edu

Dataset Overview

This dataset contains the data that was compiled for the publication Jin K, Rychahou P, Binzel DW, Evers BM, Guo P. RNA-Micelles as Self-Assembling Structures for Efficient Co-Delivery of Synergistic siRNA and Nucleoside Analogues to Treat CRC Lung Metastasis. Adv Funct Mater. 2026 Jan 20:e21863. doi: 10.1002/adfm.202521863. Epub ahead of print. PMID: 42006041; PMCID: PMC13089865. This data covers the generation and characterization of RNA nanoparticle - micelles, and evaluation to treat lung metastasis colorectal cancer tumors in murine models. This data set includes the assembly of RNA nanoparticle - micelles that are incorporated with survivin siRNA and multiple copies of gemcitabine; characterization of micelle formation by gel assembly, dynamic light scattering, zeta potential, and ultracentrifugation sedimentation. RNA nanoparticle - micelles were evaluated on HT29 lung metastasis and HCT116 colorectal cancer cell lines for specific cell uptake and targeting, survivin expression, cell viability, γH2AX and caspase3 expression, and tumor treatment by GFP expression in murine models.

Dates of Data Collection

This data was collected at The Ohio State University under Dr. Peixuan Guo and at the University of Kentucky under Dr. Piotr Rychahou between 09/01/2022 and 08/18/2025.

Funding

This work was supported by the National Cancer Institute grant R01 CA293945 (Peixuan Guo), the NIH Eye institute R01 EY031452 (Peixuan Guo), and The Ohio State University President's Research Excellence (PRE) Catalyst Award.

Ethics Approval

Animal model testing in mice was conducted under an approved IACUC protocol granted by the University of Kentucky.

Sharing/Access information

Sharing/Access

This work was published under the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. The work is copyright by the authors (© 2026 The Author(s)). Additionally, in accordance to National Institute of Health policies, this data is freely distributed.

Data Sources

All data was generated in the authors' laboratories between 2022 and 2025 through the generation of RNA nanoparticle - micelles, cell models, and animal models that are described in the methodology of the cited publication.

Recommended Citation

Jin K, Rychahou P, Binzel DW, Evers BM, Guo P. RNA-Micelles as Self-Assembling Structures for Efficient Co-Delivery of Synergistic siRNA and Nucleoside Analogues to Treat CRC Lung Metastasis. Adv Funct Mater. 2026 Jan 20:e21863.

Description of the data and file structure

This repository consists of a single file that includes multiple tabs of data in a Microsoft Excel file format for ease of reading.

Files

The files in this dataset are the original data that was used for figures in the above cited publication. These files consist of csv spreadsheets for plotted data and original uncropped images taken of gels, western blots, confocal microscopy, and whole body images of mice. All data listed with axis titles as related to the associated plot. Each file is named for the associated data in each figure, and includes metadata on the conditions used to obtain the dataset. More details on experimental procedures can be found in the associated publication.

Files and variables

File: Figure 1B.jpg

Description: Raw and uncropped image of 2% Agarose Synergel showing RNA nanoparticle - micelle assemblies. 2% Agarose Synergel in 1x TAE buffer, 100 V for 30 minutes.

Number of variables: 2

Variable Lists

• Gel lane
• Sample name (abbreviations: Scr - Scramble siRNA, SS - single stranded, Gem - Gemcitabine, MC - micelle, Sur - survivin siRNA)

Number of samples: 8

1. Scr-SS
2. Scr-CH
3. Gem-MC
4. Sur-Gem-MC
5. Sur-Gem-CH
6. Sur-AS
7. Sur-CH
8. Sur-MC

File: Figure 1C.csv

Description: Dynamic light scattering (size) and Zeta potential of assembled nanoparticles. Samples were diluted to 10 uM in PBS buffer for analysis on Malvern Zetasizer nano-ZS.

Number of metadata rows: 1

Number of header row: 2

Number of variables: 15

Number of rows: 9

Variable Lists

• Sample name (abbreviations: Scr - Scramble siRNA, SS - single stranded, Gem - Gemcitabine, MC - micelle, Sur - survivin siRNA)
• Temperature (degree C)
• Z-Ave (d.nm): Z-average; intensity-weighted, harmonic-averaged diameter (nm)
• Attenuator: Attenuator index (1-11) of Malvern Zetasizer Nano-ZS
• PDL
• Peak 1 Mean Intensity (d.nm)
• Peak 2 Mean Intensity (d.nm)
• Peak 3 Mean Intensity (d.nm)
• Scattering Angle
• Attenuator
• ZP (mV)
• Mob (umcm/Vs)
• Cond (ms/cm)
• Average Size
• Error Size
• Average Zeta
• Error Zeta

File: Figure 1D.csv

Description: Ultracentrifugation sedimentation of RNA only (1uM). 5-20% Sucrose gradient on SW55ti rotor. Spun at 40,000 RPM for overnight at 4 degree C. Samples were fractionated from the bottom and read for fluorescence (Ex: 633 nM, Em: 670 nM).

Number of metadata rows: 3

Number of header row: 1

Number of variables: 2

Number of rows: 24

Variable Lists

• Fraction number
• Fluorescence emission (670 nm)

File: Figure 1E.csv

Description: Ultracentrifugation sedimentation of RNA micelle at 100 nM. 5-20% Sucrose gradient on SW55ti rotor. Spun at 40,000 RPM for overnight at 4 degree C. Samples were fractionated from the bottom and read for fluorescence (Ex: 633 nM, Em: 670 nM). *Fraction 24 was not plotted in the published data due to a incomplete sample volume thus providing a low fluorescence signal.

Number of metadata rows: 3

Number of header row: 1

Number of variables: 2

Number of rows: 24

Variable Lists

• Fraction number
• Fluorescence emission (670 nm)

File: Figure 1F.csv

Description: Ultracentrifugation sedimentation of RNA micelle at 1 uM. 5-20% Sucrose gradient on SW55ti rotor. Spun at 40,000 RPM for overnight at 4 degree C. Samples were fractionated from the bottom and read for fluorescence (Ex: 633 nM, Em: 670 nM).

Number of metadata rows: 3

Number of header row: 1

Number of variables: 2

Number of rows: 24

Variable Lists

• Fraction number
• Fluorescence emission (670 nm)

File: Figure 2C.jpg

Description: Raw and uncropped image of 2% Agarose synergel of RNA nanoparticle - micelle assembly. 2% Agarose Synergel in 1x TAE buffer at 100V for 30 min.

Number of variables: 2

Variable Lists

• Gel lane
• Sample Name (KB - kilobase, 3WJ - three way junction, Sur - survivin siRNA, Gem - Gemcitabine, EpC - EpCAM RNA aptamer, ss - single stranded, chol - cholesterol, MC - micelle)

Number of samples: 7

1. 1KB Ladder
2. 3WJ-Sur-Gem-EpC
3. A-Sure-SS + B-Chol
4. 3WJ-Sur-EpC-MC
5. A-Sur-Gem-SS + B-Chol
6. 3WJ-Sur-Gem-EpC-MC
7. B-Chol + Sur-Gem-EpC-SS

File: Figure 3A_HCT116_37C_1hr_3WJ-Scr-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Scr-MC assembly binding to HCT116 cells. Samples were incubated at 37 degree Celsius for 1 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3A_HCT116_37C_2hr_3WJ-Scr-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Scr-MC assembly binding to HCT116 cells. Samples were incubated at 37 degree Celsius for 2 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3A_HCT116_4C_1hr_3WJ-Scr-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Scr-MC assembly binding to HCT116 cells. Samples were incubated at 4 degree Celsius for 1 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3A_HCT116_4C_2hr_3WJ-Scr-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Scr-MC assembly binding to HCT116 cells. Samples were incubated at 4 degree Celsius for 2 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3A_HCT116_37C_1hr_3WJ-Sur-EpC-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Sur-EpC-MC assembly binding to HCT116 cells. Samples were incubated at 37 degree Celsius for 1 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3A_HCT116_37C_2hr_3WJ-Scr-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Scr-MC assembly binding to HCT116 cells. Samples were incubated at 37 degree Celsius for 2 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3A_HCT116_4C_1hr_3WJ-Sur-EpC-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Sur-EpC-MC assembly binding to HCT116 cells. Samples were incubated at 4 degree Celsius for 1 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3A_HCT116_4C_2hr_3WJ-Scr-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Scr-MC assembly binding to HCT116 cells. Samples were incubated at 4 degree Celsius for 2 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3A_HT29_37C_1hr_3WJ-Scr-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Scr-MC assembly binding to HT29 cells. Samples were incubated at 37 degree Celsius for 1 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3A_HT29_37C_2hr_3WJ-Scr-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Scr-MC assembly binding to HT29 cells. Samples were incubated at 37 degree Celsius for 2 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3A_HT29_4C_1hr_3WJ-Scr-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Scr-MC assembly binding to HT29 cells. Samples were incubated at 4 degree Celsius for 1 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3A_HT29_4C_2hr_3WJ-Scr-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Scr-MC assembly binding to HT29 cells. Samples were incubated at 4 degree Celsius for 2 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3A_HT29_37C_1hr_3WJ-Sur-EpC-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Sur-EpC-MC assembly binding to HT29 cells. Samples were incubated at 37 degree Celsius for 1 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3A_HT29_37C_2hr_3WJ-Scr-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Scr-MC assembly binding to HT29 cells. Samples were incubated at 37 degree Celsius for 2 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3A_HT29_4C_1hr_3WJ-Sur-EpC-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Sur-EpC-MC assembly binding to HT29 cells. Samples were incubated at 4 degree Celsius for 1 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3A_HT29_4C_2hr_3WJ-Scr-MC.tif

Description: Raw and uncropped microscopy image of 3WJ-Scr-MC assembly binding to HT29 cells. Samples were incubated at 4 degree Celsius for 2 hour prior to imaging at 20X magnification. Blue stain for DAPI, Red Stain for RNA nanoparticle.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - Alexa647 label on RNA micelle

File: Figure 3B_HCT116_24hr_5x_3WJ-Sur-Epc.tif

Description: Raw and uncropped microscopy image of survivin staining in HCT116 cells following incubation with 3WJ-Sur-EpC nanoparticle. Cells were incubated with RNA nanoparticles for 24 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3B_HCT116_24hr_20x_3WJ-Sur-Epc.tif

Description: Raw and uncropped microscopy image of survivin staining in HCT116 cells following incubation with 3WJ-Sur-EpC nanoparticle. Cells were incubated with RNA nanoparticles for 24 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3B_HCT116_24hr_5x_3WJ-Sur-Epc-MC.tif

Description: Raw and uncropped microscopy image of survivin staining in HCT116 cells following incubation with 3WJ-Sur-EpC-MC micelles. Cells were incubated with RNA nanoparticles for 24 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3B_HCT116_24hr_20x_3WJ-Sur-Epc-MC.tif

Description: Raw and uncropped microscopy image of survivin staining in HCT116 cells following incubation with 3WJ-Sur-EpC-MC micelles. Cells were incubated with RNA nanoparticles for 24 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3B_HCT116_48hr_5x_3WJ-Sur-Epc.tif

Description: Raw and uncropped microscopy image of survivin staining in HCT116 cells following incubation with 3WJ-Sur-EpC nanoparticle. Cells were incubated with RNA nanoparticles for 48 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3B_HCT116_48hr_20x_3WJ-Sur-Epc.tif

Description: Raw and uncropped microscopy image of survivin staining in HCT116 cells following incubation with 3WJ-Sur-EpC nanoparticle. Cells were incubated with RNA nanoparticles for 48 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3B_HCT116_48hr_5x_3WJ-Sur-Epc-MC.tif

Description: Raw and uncropped microscopy image of survivin staining in HCT116 cells following incubation with 3WJ-Sur-EpC-MC micelles. Cells were incubated with RNA nanoparticles for 48 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3B_HCT116_48hr_20x_3WJ-Sur-Epc-MC.tif

Description: Raw and uncropped microscopy image of survivin staining in HCT116 cells following incubation with 3WJ-Sur-EpC-MC micelles. Cells were incubated with RNA nanoparticles for 48 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3B_HT29_24hr_5x_3WJ-Sur-Epc.tif

Description: Raw and uncropped microscopy image of survivin staining in HT29 cells following incubation with 3WJ-Sur-EpC nanoparticle. Cells were incubated with RNA nanoparticles for 24 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3B_HT29_24hr_20x_3WJ-Sur-Epc.tif

Description: Raw and uncropped microscopy image of survivin staining in HT29 cells following incubation with 3WJ-Sur-EpC nanoparticle. Cells were incubated with RNA nanoparticles for 24 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3B_HT29_24hr_5x_3WJ-Sur-Epc-MC.tif

Description: Raw and uncropped microscopy image of survivin staining in HT29 cells following incubation with 3WJ-Sur-EpC-MC micelles. Cells were incubated with RNA nanoparticles for 24 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3B_HT29_24hr_20x_3WJ-Sur-Epc-MC.tif

Description: Raw and uncropped microscopy image of survivin staining in HT29 cells following incubation with 3WJ-Sur-EpC-MC micelles. Cells were incubated with RNA nanoparticles for 24 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3B_HT29_48hr_5x_3WJ-Sur-Epc.tif

Description: Raw and uncropped microscopy image of survivin staining in HT29 cells following incubation with 3WJ-Sur-EpC nanoparticle. Cells were incubated with RNA nanoparticles for 48 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3B_HT29_48hr_20x_3WJ-Sur-Epc.tif

Description: Raw and uncropped microscopy image of survivin staining in HT29 cells following incubation with 3WJ-Sur-EpC nanoparticle. Cells were incubated with RNA nanoparticles for 48 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3B_HT29_48hr_5x_3WJ-Sur-Epc-MC.tif

Description: Raw and uncropped microscopy image of survivin staining in HT29 cells following incubation with 3WJ-Sur-EpC-MC micelles. Cells were incubated with RNA nanoparticles for 48 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3B_HT29_48hr_20x_3WJ-Sur-Epc-MC.tif

Description: Raw and uncropped microscopy image of survivin staining in HT29 cells following incubation with 3WJ-Sur-EpC-MC micelles. Cells were incubated with RNA nanoparticles for 48 hours at 37 degree Celsius then incubated with survivin (71G4B7) rabbit monoclonal antibody overnight at 4°C, washed with PBS, and incubated with VectaFluor Anti-Rabbit secondary antibodies (red) for 30 min at 37°C and stained with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 3C_Actin.tif

Description: Raw and uncropped image of B-actin expression by western blot in HT29 cells following treatment with RNA nanoparticle -micelles.

Number of variables: 2

Variable Lists

• Lane
• Sample name (Sur - survivin siRNA, DNAr - DNA surivivn anti-sense strand, MC - Micelle, Gem - Gemcitabine)

Number of samples: 8

1. No treatment control
2. 200 nM Sur-Gem-MC
3. 100 nM Sur-Gem-MC
4. 50 nM Sur-Gem-MC
5. 200 nM Sur-DNAr-Gem-MC
6. 1200 nM Sur-DNAr-Gem-MC
7. 50 nM Sur-DNAr-Gem-MC
8. 200 nM Sur

File: Figure 3C_Survivin.tif

Description: Raw and uncropped image of survivin expression by western blot in HT29 cells following treatment with RNA nanoparticle -micelles.

Number of variables: 2

Variable Lists

• Lane
• Sample name (Sur - survivin siRNA, DNAr - DNA surivivn anti-sense strand, MC - Micelle, Gem - Gemcitabine)

Number of samples: 8

1. No treatment control
2. 200 nM Sur-Gem-MC
3. 100 nM Sur-Gem-MC
4. 50 nM Sur-Gem-MC
5. 200 nM Sur-DNAr-Gem-MC
6. 1200 nM Sur-DNAr-Gem-MC
7. 50 nM Sur-DNAr-Gem-MC
8. 200 nM Sur

File: Figure 4A.csv

Description: Cell viability assay of HT29 or HCT116 cell lines following incubation with RNA nanoparticle -micelles. HT29 or HCT116 treated with RNA nanoparticles at 25 nM for 96 hours or HCT116 treated with RNA nanoparticles at 10 nM for 96 hours. Following incubation assay was completed and absorbance was measured at 460 nm. Additional image of the cell assay is included as separate files (Figure 4A_HT29 and Figure 4A_HCT116). The cell viability average and standard error were used to create a plot in the publication.

Number of metadata rows: 2

Number of header row: 2

Number of variables: 11

Number of rows: 14

Variable Lists

• Sample name (Sur - survivin siRNA, DNAr - DNA surivivn anti-sense strand, MC - Micelle, Gem - Gemcitabine)
• Absorbance measurement of repeat 1 (460 nm)
• Absorbance measurement of repeat 2 (460 nm)
• Absorbance measurement of repeat 3 (460 nm)
• Average of absorbance measurements (repeat 1-3)
• Standard error of absorbance measurements (repeat 1-3)
• Cell viability repeat 1 (expressed as percentage of absorbance repeat/absorbance average)
• Cell viability repeat 2 (expressed as percentage of absorbance repeat/absorbance average)
• Cell viability repeat 3 (expressed as percentage of absorbance repeat/absorbance average)
• Cell viability average (repeat 1-3)
• Standard error of cell viability average (repeat 1-3)

File: Figure 4A_HCT116.tif

Description: Raw and uncropped image of cell viability assay of HCT116 cells following incuation with RNA micelles. Image accompanies Figure 4A.csv file. Each column represents a different sample with rows 1-3 serving as repeats at 10 nM.

Number of variables: 2

Variable Lists

• Lane
• Sample name (Sur - survivin siRNA, EpC-EpCAM RNA aptamer, MC - Micelle, Gem - Gemcitabine)

Number of samples: 7 (columns)

1. PBS
2. Gem-MC
3. Sur-MC
4. Sur-Gem-MC
5. Scr-MC
6. 3WJ-Sur-EpC-MC
7. 3WJ-Sur-Gem-EpC-MC

File: Figure 4A_HT29.tif

Description: Raw and uncropped image of cell viability assay of HT29 cells following incuation with RNA micelles. Image accompanies Figure 4A.csv file. Each column represents a different sample with rows 1-3 serving as repeats at 25 nM.

Number of variables: 2

Variable Lists

• Lane
• Sample name (Sur - survivin siRNA, EpC-EpCAM RNA aptamer, MC - Micelle, Gem - Gemcitabine)

Number of samples: 7 (columns)

1. PBS
2. Gem-MC
3. Sur-MC
4. Sur-Gem-MC
5. Scr-MC
6. 3WJ-Sur-EpC-MC
7. 3WJ-Sur-Gem-EpC-MC

File: Figure 4B.csv

Description: Cell viability assay of HT29 or HCT116 cell lines following incubation with RNA nanoparticle -micelles. HT29 or HCT116 cells treated with RNA nanoparticles at 200 nM for 180 minutes then replaced with cell media 96 hours. Following incubation assay was completed and absorbance was measured at 460 nm. Additional image of the cell assay is included as separate files (Figure 4B_HT29 and Figure 4B_HCT116). The cell viability average and standard error were used to create a plot in the publication.

Number of metadata rows: 2

Number of header row: 2

Number of variables: 11

Number of rows: 14

Variable Lists

• Sample name (Sur - survivin siRNA, DNAr - DNA surivivn anti-sense strand, MC - Micelle, Gem - Gemcitabine)
• Absorbance measurement of repeat 1 (460 nm)
• Absorbance measurement of repeat 2 (460 nm)
• Absorbance measurement of repeat 3 (460 nm)
• Average of absorbance measurements (repeat 1-3)
• Standard error of absorbance measurements (repeat 1-3)
• Cell viability repeat 1 (expressed as percentage of absorbance repeat/absorbance average)
• Cell viability repeat 2 (expressed as percentage of absorbance repeat/absorbance average)
• Cell viability repeat 3 (expressed as percentage of absorbance repeat/absorbance average)
• Cell viability average (repeat 1-3)
• Standard error of cell viability average (repeat 1-3)

File: Figure 4B_HCT116.tif

Description: Raw and uncropped image of cell viability assay of HCT116 cells following incuation with RNA micelles at 200 nM for 180 min and then replaced with full medium for 96 hour. Image accompanies Figure 4b.csv file. Each column represents a different sample with rows 1-3 serving as repeats.

Number of variables: 2

Variable Lists

• Lane
• Sample name (Sur - survivin siRNA, EpC-EpCAM RNA aptamer, MC - Micelle, Gem - Gemcitabine)

Number of samples: 7 (columns)

1. PBS
2. Gem-MC
3. Sur-MC
4. Sur-Gem-MC
5. Scr-MC
6. 3WJ-Sur-EpC-MC
7. 3WJ-Sur-Gem-EpC-MC

File: Figure 4B_HT29.tif

Description: Raw and uncropped image of cell viability assay of HT29 cells following incuation with RNA micelles at 200 nM for 180 min and then replaced with full medium for 96 hour. Image accompanies Figure 4b.csv file. Each column represents a different sample with rows 1-3 serving as repeats.

Number of variables: 2

Variable Lists

• Lane
• Sample name (Sur - survivin siRNA, EpC-EpCAM RNA aptamer, MC - Micelle, Gem - Gemcitabine)

Number of samples: 7 (columns)

1. PBS
2. Gem-MC
3. Sur-MC
4. Sur-Gem-MC
5. Scr-MC
6. 3WJ-Sur-EpC-MC
7. 3WJ-Sur-Gem-EpC-MC

File: Figure 4C_HCT116_3WJ-Sur-EpC-MC.tif

Description: Raw and uncropped image of γH2AX expression by confocal microscopy in HCT116 cells following treatment with 3WJ-Sur-EpC-MC micelles. Cells were treated with 100 nm RNA‑micelles for 24 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (γH2AX (Ser139) mouse monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑mouse, red) and with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-mouse antibody

File: Figure 4C_HCT116_3WJ-Sur-Gem-EpC-MC.tif

Description: Raw and uncropped image of γH2AX expression by confocal microscopy in HCT116 cells following treatment with 3WJ-Sur-Gem-EpC-MC micelles. Cells were treated with 100 nm RNA‑micelles for 24 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (γH2AX (Ser139) mouse monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑mouse, red) and with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-mouse antibody

File: Figure 4C_HCT116_Gem-MC.tif

Description: Raw and uncropped image of γH2AX expression by confocal microscopy in HCT116 cells following treatment with Gem-MC micelles. Cells were treated with 100 nm RNA‑micelles for 24 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (γH2AX (Ser139) mouse monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑mouse, red) and with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-mouse antibody

File: Figure 4C_HCT116_PBS.tif

Description: Raw and uncropped image of γH2AX expression by confocal microscopy in HCT116 cells following treatment with PBS. Cells were treated with 100 nm RNA‑micelles for 24 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (γH2AX (Ser139) mouse monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑mouse, red) and with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-mouse antibody

File: Figure 4C_HCT116_Scr-MC.tif

Description: Raw and uncropped image of γH2AX expression by confocal microscopy in HCT116 cells following treatment with Scr-MC micelles. Cells were treated with 100 nm RNA‑micelles for 24 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (γH2AX (Ser139) mouse monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑mouse, red) and with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-mouse antibody

File: Figure 4C_HCT116_Sur-Gem-MC.tif

Description: Raw and uncropped image of γH2AX expression by confocal microscopy in HCT116 cells following treatment with Sur-Gem-MC micelles. Cells were treated with 100 nm RNA‑micelles for 24 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (γH2AX (Ser139) mouse monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑mouse, red) and with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-mouse antibody

File: Figure 4C_HCT116_Sur-MC.tif

Description: Raw and uncropped image of γH2AX expression by confocal microscopy in HCT116 cells following treatment with Sur-MC micelles. Cells were treated with 100 nm RNA‑micelles for 24 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (γH2AX (Ser139) mouse monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑mouse, red) and with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-mouse antibody

File: Figure 4C_HT29_3WJ-Sur-EpC-MC.tif

Description: Raw and uncropped image of γH2AX expression by confocal microscopy in HT29 cells following treatment with 3WJ-Sur-EpC-MC micelles. Cells were treated with 100 nm RNA‑micelles for 24 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (γH2AX (Ser139) mouse monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑mouse, red) and with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-mouse antibody

File: Figure 4C_HT29_3WJ-Sur-Gem-EpC-MC.tif

Description: Raw and uncropped image of γH2AX expression by confocal microscopy in HT29 cells following treatment with 3WJ-Sur-Gem-EpC-MC micelles. Cells were treated with 100 nm RNA‑micelles for 24 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (γH2AX (Ser139) mouse monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑mouse, red) and with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-mouse antibody

File: Figure 4C_HT29_Gem-MC.tif

Description: Raw and uncropped image of γH2AX expression by confocal microscopy in HT29 cells following treatment with Gem-MC micelles. Cells were treated with 100 nm RNA‑micelles for 24 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (γH2AX (Ser139) mouse monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑mouse, red) and with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-mouse antibody

File: Figure 4C_HT29_PBS.tif

Description: Raw and uncropped image of γH2AX expression by confocal microscopy in HT29 cells following treatment with PBS. Cells were treated with 100 nm RNA‑micelles for 24 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (γH2AX (Ser139) mouse monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑mouse, red) and with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-mouse antibody

File: Figure 4C_HT29_Scr-MC.tif

Description: Raw and uncropped image of γH2AX expression by confocal microscopy in HT29 cells following treatment with Scr-MC micelles. Cells were treated with 100 nm RNA‑micelles for 24 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (γH2AX (Ser139) mouse monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑mouse, red) and with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-mouse antibody

File: Figure 4C_HT29_Sur-Gem-MC.tif

Description: Raw and uncropped image of γH2AX expression by confocal microscopy in HT29 cells following treatment with Sur-Gem-MC micelles. Cells were treated with 100 nm RNA‑micelles for 24 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (γH2AX (Ser139) mouse monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑mouse, red) and with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-mouse antibody

File: Figure 4C_HT29_Sur-MC.tif

Description: Raw and uncropped image of γH2AX expression by confocal microscopy in HT29 cells following treatment with Sur-MC micelles. Cells were treated with 100 nm RNA‑micelles for 24 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (γH2AX (Ser139) mouse monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑mouse, red) and with DAPI. Samples were imaged at 20x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-mouse antibody

File: Figure 4D_HCT116_3WJ-Sur-EpC-MC.tif

Description: Raw and uncropped image of cleaved caspase‑3 expression by confocal microscopy in HCT116 cells following treatment with 3WJ-Sur-EpC-MC micelles. Cells were treated with 100 nm RNA‑micelles for 48 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (cleaved caspase‑3 (Asp175) rabbit monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑rabbit, red) and with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 4D_HCT116_3WJ-Sur-Gem-EpC-MC.tif

Description: Raw and uncropped image of cleaved caspase‑3 expression by confocal microscopy in HCT116 cells following treatment with 3WJ-Sur-Gem-EpC-MC micelles. Cells were treated with 100 nm RNA‑micelles for 48 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (cleaved caspase‑3 (Asp175) rabbit monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑rabbit, red) and with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 4D_HCT116_Gem-MC.tif

Description: Raw and uncropped image of cleaved caspase‑3 expression by confocal microscopy in HCT116 cells following treatment with Gem-MC micelles. Cells were treated with 100 nm RNA‑micelles for 48 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (cleaved caspase‑3 (Asp175) rabbit monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑rabbit, red) and with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 4D_HCT116_PBS.tif

Description: Raw and uncropped image of cleaved caspase‑3 expression by confocal microscopy in HCT116 cells following treatment with PBS. Cells were treated with 100 nm RNA‑micelles for 48 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (cleaved caspase‑3 (Asp175) rabbit monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑rabbit, red) and with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 4D_HCT116_Scr-MC.tif

Description: Raw and uncropped image of cleaved caspase‑3 expression by confocal microscopy in HCT116 cells following treatment with Scr-MC micelles. Cells were treated with 100 nm RNA‑micelles for 48 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (cleaved caspase‑3 (Asp175) rabbit monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑rabbit, red) and with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 4D_HCT116_Sur-Gem-MC.tif

Description: Raw and uncropped image of cleaved caspase‑3 expression by confocal microscopy in HCT116 cells following treatment with Sur-Gem-MC micelles. Cells were treated with 100 nm RNA‑micelles for 48 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (cleaved caspase‑3 (Asp175) rabbit monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑rabbit, red) and with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 4D_HCT116_Sur-MC.tif

Description: Raw and uncropped image of cleaved caspase‑3 expression by confocal microscopy in HCT116 cells following treatment with Sur-MC micelles. Cells were treated with 100 nm RNA‑micelles for 48 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (cleaved caspase‑3 (Asp175) rabbit monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑rabbit, red) and with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 4D_HT29_3WJ-Sur-EpC-MC.tif

Description: Raw and uncropped image of cleaved caspase‑3 expression by confocal microscopy in HT29 cells following treatment with 3WJ-Sur-EpC-MC micelles. Cells were treated with 100 nm RNA‑micelles for 48 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (cleaved caspase‑3 (Asp175) rabbit monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑rabbit, red) and with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 4D_HT29_3WJ-Sur-Gem-EpC-MC.tif

Description: Raw and uncropped image of cleaved caspase‑3 expression by confocal microscopy in HT29 cells following treatment with 3WJ-Sur-Gem-EpC-MC micelles. Cells were treated with 100 nm RNA‑micelles for 48 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (cleaved caspase‑3 (Asp175) rabbit monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑rabbit, red) and with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 4D_HT29_Gem-MC.tif

Description: Raw and uncropped image of cleaved caspase‑3 expression by confocal microscopy in HT29 cells following treatment with Gem-MC micelles. Cells were treated with 100 nm RNA‑micelles for 48 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (cleaved caspase‑3 (Asp175) rabbit monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑rabbit, red) and with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 4D_HT29_PBS.tif

Description: Raw and uncropped image of cleaved caspase‑3 expression by confocal microscopy in HT29 cells following treatment with PBS. Cells were treated with 100 nm RNA‑micelles for 48 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (cleaved caspase‑3 (Asp175) rabbit monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑rabbit, red) and with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 4D_HT29_Scr-MC.tif

Description: Raw and uncropped image of cleaved caspase‑3 expression by confocal microscopy in HT29 cells following treatment with Scr-MC micelles. Cells were treated with 100 nm RNA‑micelles for 48 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (cleaved caspase‑3 (Asp175) rabbit monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑rabbit, red) and with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 4D_HT29_Sur-Gem-MC.tif

Description: Raw and uncropped image of cleaved caspase‑3 expression by confocal microscopy in HT29 cells following treatment with Sur-Gem-MC micelles. Cells were treated with 100 nm RNA‑micelles for 48 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (cleaved caspase‑3 (Asp175) rabbit monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑rabbit, red) and with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 4D_HT29_Sur-MC.tif

Description: Raw and uncropped image of cleaved caspase‑3 expression by confocal microscopy in HT29 cells following treatment with Sur-MC micelles. Cells were treated with 100 nm RNA‑micelles for 48 hours then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X‑100. After blocking, samples were incubated overnight at 4°C with primary antibody (cleaved caspase‑3 (Asp175) rabbit monoclonal) and stained with VectaFluor‑conjugated secondary antibody (anti‑rabbit, red) and with DAPI. Samples were imaged at 5x magnification.

Number of variables: 2

Variable Lists

• Blue Stain - DAPI nucleus stain
• Red Stain - VectaFluor Anti-Rabbit antibody

File: Figure 5A_3WJ-Sur-EpC-MC.tiff

Description: Raw and uncropped image of whole mouse bioluminescence expression from HT29 LungM3 cancer cells by IVIS following treatment with 3WJ-Sur-EpC-MC. This image include 5 mouse repeat for the sample. Reduced bioluminescence expression represents treatment of HT29 LungM3 tumors developed on lungs of mice. Images were taken in white light to image mice and bioluminesnce to image tumor cells.

Number of variables: 2

Variable List:

• Bioluminescence
• White light

File: Figure 5A_3WJ-Sur-Gem-EpC-MC.tiff

Description: Raw and uncropped image of whole mouse bioluminescence expression from HT29 LungM3 cancer cells by IVIS following treatment with 3WJ-Sur-Gem-EpC-MC. This image include 5 mouse repeat for the sample. Reduced bioluminescence expression represents treatment of HT29 LungM3 tumors developed on lungs of mice. Images were taken in white light to image mice and bioluminesnce to image tumor cells.

Number of variables: 2

Variable List:

• Bioluminescence
• White light

File: Figure 5A_Gem-MC.tiff

Description: Raw and uncropped image of whole mouse bioluminescence expression from HT29 LungM3 cancer cells by IVIS following treatment with Gem-MC. This image include 5 mouse repeat for the sample. Reduced bioluminescence expression represents treatment of HT29 LungM3 tumors developed on lungs of mice. Images were taken in white light to image mice and bioluminesnce to image tumor cells.

Number of variables: 2

Variable List:

• Bioluminescence
• White light

File: Figure 5A_PBS.tiff

Description: Raw and uncropped image of whole mouse bioluminescence expression from HT29 LungM3 cancer cells by IVIS following treatment with PBS. This image include 5 mouse repeat for the sample. Reduced bioluminescence expression represents treatment of HT29 LungM3 tumors developed on lungs of mice. Images were taken in white light to image mice and bioluminesnce to image tumor cells.

Number of variables: 2

Variable List:

• Bioluminescence
• White light

File: Figure 5A_Scr-MC.tiff

Description: Raw and uncropped image of whole mouse bioluminescence expression from HT29 LungM3 cancer cells by IVIS following treatment with Scr-MC. This image include 5 mouse repeat for the sample. Reduced bioluminescence expression represents treatment of HT29 LungM3 tumors developed on lungs of mice. Images were taken in white light to image mice and bioluminesnce to image tumor cells.

Number of variables: 2

Variable List:

• Bioluminescence
• White light

File: Figure 5A_Sur-Gem-MC.tiff

Description: Raw and uncropped image of whole mouse bioluminescence expression from HT29 LungM3 cancer cells by IVIS following treatment with Sur-Gem-MC. This image include 5 mouse repeat for the sample. Reduced bioluminescence expression represents treatment of HT29 LungM3 tumors developed on lungs of mice. Images were taken in white light to image mice and bioluminesnce to image tumor cells.

Number of variables: 2

Variable List:

• Bioluminescence
• White light

File: Figure 5A_Sur-MC.tiff

Description: Raw and uncropped image of whole mouse bioluminescence expression from HT29 LungM3 cancer cells by IVIS following treatment with Sur-MC. This image include 5 mouse repeat for the sample. Reduced bioluminescence expression represents treatment of HT29 LungM3 tumors developed on lungs of mice. Images were taken in white light to image mice and bioluminesnce to image tumor cells.

Number of variables: 2

Variable List:

• Bioluminescence
• White light

File: Figure 5B.csv

Description: Quantification of fluorescence of lungs isolated from mice with GFP expression from HT29 LungM3 cancer cells by IVIS following treatment with RNA nanoparticle -micelles.

Number of metadata rows: 2

Number of header row: 1

Number of variables: 2

Number of rows: 7 (each row is animal repeat, followed by average and standard error)

Variable List:

• Sample name (Sur - survivin siRNA, 3WJ - three way junction, EpC - EpCAM RNA aptamer, MC - Micelle, Gem - Gemcitabine)
• Tumor repeat

File: Figure 5B.jpg

Description: Raw and uncropped image of GFP expression from HT29 LungM3 cells in lungs of mice. Mice were treated with RNA nanoparticle micelles, mice were sacrifice and lungs were disected. Fluoresence signal is GFP expression by tumor cells. Each row is a different RNA micelle sample with columns representing animal repeats.

Number of variables: 2

Variable Lists

• Animal Repeat number
• Sample name (abbreviations: Scr - Scramble siRNA, SS - single stranded, Gem - Gemcitabine, MC - micelle, Sur - survivin siRNA)

Number of samples (row): 7

1. PBS
2. Gem-MC
3. Sur-MC
4. Sur-Gem-MC
5. Scr-MC
6. 3WJ-Sur-EpC-MC
7. 3WJ-Sur-Gem-EpC-MC

File: Figure 5C.csv

Description: Quantification of fluorescence of whole mouse with bioluminescence expression from HT29 LungM3 cancer cells by IVIS following treatment with RNA nanoparticle -micelles.

Number of metadata rows: 2

Number of header row: 1

Number of variables: 2

Number of rows: 7 (each row is animal repeat, followed by average and standard error)

Variable List:

• Sample name (Sur - survivin siRNA, 3WJ - three way junction, EpC - EpCAM RNA aptamer, MC - Micelle, Gem - Gemcitabine)
• Tumor repeat

Code/software

This data consists of images (.jpg, .tif, .tiff) that can be opened in any imaging software, and comma delimited (.csv) spreadsheets.