Data from: Electrochemical sensing of hormonal serotonin levels in Crayfish
Data files
Dec 24, 2025 version files 126.47 KB
-
ANOVA_Serotonin_Overton.sav
2.22 KB
-
Data_IsolateCommunal.xlsx
41.73 KB
-
KW_MWU_Serotonin_Overton.sav
1.40 KB
-
MWU_Feeding_Sex_Overton.sav
2.49 KB
-
README.md
3.58 KB
-
Stats_Output_SPSS_2_Overton.spv
18.12 KB
-
Stats_Output_SPSS_Overton.spv
56.94 KB
Abstract
The neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) has been implicated as a key biomarker that regulates a range of neurobehavioral functions. 5-HT also plays a prominent role in modulating social behavior across species. Here, we report the successful differentiation of hormonal (circulatory) 5-HT concentrations in socially isolated and communal crayfish samples by a surface-modified carbon fiber microelectrode electrochemical sensor. Monitoring 5-HT concentrations across social conditions provided important insights into mechanisms linking isolation to neurohormones that guide behavior. Utilizing surface coatings and electrochemical etching, our sensor can detect endogenous, nanomolar concentrations of 5-HT in vitro from crayfish hemolymph (i.e., blood) collected from communally housed animals and those isolated for one or seven days. Our measurements revealed that crayfish responses to isolation varied by species: Procambarus crayfish isolated for seven days exhibited a major increase in 5-HT compared to communally housed or short-term isolated conspecifics, whereas for Faxonius crayfish, the hormonal concentrations of 5-HT peaked after one day of isolation but paralleled the levels of communally housed crayfish after one week of isolation. These findings underscore the complex nature of neurochemical pathways in response to social conditions, demonstrate the sensor's utility for studying 5-HT dynamics, and provide a method for monitoring temporal changes in hormonal 5-HT following social isolation.
Dataset DOI: 10.5061/dryad.r2280gbs6
Description of the data and file structure
The excel sheet consists of raw data including the 5-HT concentration, Ipa values, weight, sex, label, social condition, and feeding conditions. The excel sheet lists all the values compiled and sorted for easier access. The first three tabs of the excel sheet includes the statistical output and input of the dataset, including ANOVA test, Kruskal-Wallis, and Mann-Whitney U.
Multiple files in this repository require SPSS to generate. The SPSS files contain the raw input data for each test as well and statistical outputs.
Files and variables
File: Data_IsolateCommunal.xlsx
Description: Sorted raw data sheet including every experimental variable described below.
Variables
- Data sorted by social condition, weight (in grams), sex, Ipa (in µA), 5-HT concentration (in nM), feeding condition, linear regression coefficient, codetection calibration (Ipa (in µA) and concentration (in nM), and statistical outputs.
- To Calculate Concentration from Current Response, use a current response value and a linear regression model (A = slope, B = intercept) to compute concentration:
Concentration = (Ipa – B) / A. - Dates listed as mm/dd/yyyy.
- Empty cells in the ANOVA Statistical Results tab is due to inapplicable values. The cells are filled according to the statistical output.
File: KW_MWU_Serotonin_Overton.sav
Description: Input data to generate Kruskal-Wallis and Mann-Whitney U statistical outputs for species and social conditions. The variables include Procambarus clarkii serotonin concentration (in nM), Procambarus clarkii social condition (COMs, denoted by 1, 1-day ISOs, denoted by 2, and 7-day ISOs, denoted by 3), Faxonius serotonin concentration (in nM), and Faxonius social condition (COMs, denoted by 1, 1-day ISOs, denoted by 2, and 7-day ISOs, denoted by 3).
File: ANOVA_Serotonin_Overton.sav
Description: Input data to generate Kruskal-Wallis and Mann-Whitney U statistical outputs for species and social conditions. The variables include species (Procambarus clarkii or Faxonius), social condition (COMs, 1-day ISOs, 7-day ISOs), and serotonin (in nM).
File: MWU_Feeding_Sex_Overton.sav
Description: Input data to generate Mann-Whitney U statistical outputs for sex and weight differences and feeding conditions. The variables include weight (in grams), sex (females, denoted by 1, and males, denoted by 2), serotonin concentration for fed animals (in nM), serotonin concentration for all animals (in nM), and species (Procambarus clarkii, denoted by 1 or Faxonius, denoted by 2).
File: Stats_Output_SPSS_2_Overton.spv
Description: Statistical data outputs for feeding condition as well as the sex and weight differences.
File: Stats_Output_SPSS_Overton.spv
Description: Statistical data outputs for species and social condition differences.
Code/software
The .sav and .spv files were generated using SPSS software. Use recent versions (V31 or earlier) to open the analysis outputs. If access to the software if unavailable, .sav files can be opened using, JASP, an open-source software. The .spv files can be viewed using an open-source software, PSPP.
Access information
Other publicly accessible locations of the data:
- N/A
Data was derived from the following sources:
- N/A
All statistical analyses were performed using IBM SPSS (IBM Corp.). Ipa data is represented as the mean of measurements from a sample (n=3). Samples where no 5-HT was detected (Ipa=0 µA) were excluded from statistical analysis. Statistical comparisons among social groups and species were completed using a one-wayanalysis of variance (ANOVA). Differences between species were analyzed using non-parametric Kruskal-Wallis tests, followed by Mann-Whitney U tests for pair-wise comparison. Single-variable cross-species groups were evaluated using Mann-Whitney U tests. Statistical significance was defined at p<0.05.