RNA-Seq profiles of bortezomib-sensitive and -resistant multiple myeloma in bone marrow samples from African American patients
Data files
Mar 25, 2026 version files 24.52 MB
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geneDE_results.csv
24.52 MB
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README.md
2 KB
Abstract
This study aimed to identify circulating microRNAs (miRNAs) associated with bortezomib resistance in multiple myeloma (MM) among African American males using RNA sequencing. RNA-Seq was conducted on 10 bone marrow samples (5 bortezomib-sensitive and 5 bortezomib-resistant) from African American males. miRNA extraction was performed using the phenol-chloroform method with TRIzol reagent (Invitrogen). RNA-Seq and subsequent bioinformatics analyses were carried out by CD Genomics using standard protocols.
Dataset DOI: 10.5061/dryad.rbnzs7hss
Description of the data and file structure
This dataset includes ncRNA data isolated from bone marrow samples of 10 African American patients, including 5 Bortezomib-resistant and 5 Bortezomib-sensitive samples. The findings suggested that circulating miR-18a-5p and miR-20a-5p were identified as key biomarkers associated with bortezomib resistance in African American males with multiple myeloma. Differential expression and technical confirmation of these miRNAs in resistant and sensitive bone marrow samples were performed using RNA-seq and qRT-PCR. Results support the potential clinical utility of these circulating miR-18a-5p and miR-20a-5p for diagnosing and predicting bortezomib resistance in multiple myeloma.
Files and variables
File: geneDE_results.csv
Description: RNA-seq results from bortezomib-resistant and sensitive samples from African American patients with multiple myeloma.
Variables
- Row.names: The transcript names
- baseMean: The average normalized expression level of a gene across all samples
- log2FoldChange: The log base 2 of the fold change between two conditions, bortezomib resistant and bortezomib-sensitive
- lfcSE: The standard error of the estimated log2 fold change
- stat: The test statistic used to assess differential expression
- pvalue: The probability of observing the data assuming no real difference between conditions
- padj: The p-value corrected for multiple testing
- S1: sensitive sample 1
- S2: sensitive sample 2
- S3: sensitive sample 3
- S4: sensitive sample 4
- S5: sensitive sample 5
- R6: resistant sample 6
- R7: resistant sample 7
- R8: resistant sample 8
- R9: resistant sample 9
- R10: resistant sample 10
Note: "NA" cells mean missing data or inapplicable.
Code/software
MS Excel
Bone marrow samples were collected from ten African American male multiple myeloma patients—five bortezomib (BTZ)-sensitive and five BTZ-resistant, classified by clinical response.
Total RNA enriched for microRNA was extracted using TRIzol™ (Invitrogen) per manufacturer’s instructions, and quality was verified before sequencing.
RNA-Seq library preparation, sequencing, and bioinformatics analysis were conducted by CD Genomics laboratory using standard protocols (https://www.cd-genomics.com/resouce-rna-seq-library-preparation-principles-protocol.html). Processed data included normalized expression matrices for downstream differential analysis.