Related publication: Kundu DK, Kou M, Gadd J, et al. Exosomal NAMPT from Engineered Mesenchymal Stem Cells Attenuates Aortic Stenosis by Inhibiting Endothelial-to-Mesenchymal Transition. International Journal of Molecular Sciences. 2026;27(1):256.

This dataset contains the numerical source data used to generate the figures presented in the associated publication. The study investigated whether exosomes derived from mesenchymal stem cells (MSCs) engineered to overexpress nicotinamide phosphoribosyltransferase (NAMPT) could reduce the progression of aortic stenosis in an endothelial cell-specific CXCR4 knockout mouse model. The data were generated from both mouse experiments and cultured endothelial cells.

Description of files and variables

Figure_1.csv

This file contains source data for Figure 1, Quantification of NAMPT expression (n = 4); expression was normalized to β-actin. Unpaired Student’s t test; ** p < 0.01 for CXCR4fl/fl vs. EC CXCR4 KO mice.

Variables include:

• Experimental group (CXCR4fl/fl, EC CXCR4 KO)
• Biological replicate number
• NAMPT and β-actin quantification values corresponding to the figure panels
• Relative NAMPT protein expression

Figure_2C.csv

This file contains source data for Figure 2C. NAMPT activity assay in immunoprecipitated lysates from NAMPT-overexpressing MSCs and control MSCs.

Variables include:

• Experimental group (NAMPT Overexpressed MSC, Control MSC)
• Reaction time (minutes)
• Quantitative fluorescence measurements of NAMPT enzymatic activity (relative signal intensity) at each time point for each experimental group

Figure_3(B-F).csv

This file contains echocardiographic measurements demonstrating the therapeutic effects of NAMPT-enriched exosomes on aortic stenosis progression. Endothelial-specific CXCR4 knockout mice with established aortic stenosis received PBS, control MSC-derived exosomes, or NAMPT-enriched MSC-derived exosomes by intraperitoneal injection once weekly for three weeks. Cardiac function was measured by serial echocardiography.

Tables within this spreadsheet are organized by experimental group (EC CXCR4 KO+ PBS, EC CXCR4 KO+ NAMPT-Exo, or EC CXCR4 KO+ MSC Exo) and measurement type.

Variables include:

• Mouse identification number
• Experimental group
• Time point
• Measurements:
  • Left ventricular internal diameter during diastole (LVID;d, mm)
  • Left ventricular posterior wall diameter during diastole (LVPW;d, mm)
  • Left ventricular internal diameter during systole (LVID;s, mm)
  • Left ventricular posterior wall diameter during systole (LVPW;s, mm)
  • Left ventricular anterior wall diameter during diastole (LVAW;d, mm)
  • Left ventricular anterior wall diameter during systole (LVAW;s, mm)
  • Left ventricular end-diastolic volume (LV Vol;d, ul)
  • Left ventricular end-systolic volume (LV Vol;s, ul)
  • Left ventricular ejection fraction (EF, %)
  • Fractional shortening (FS, %)
  • LV Mass (AW)
  • LV Mass (AW) Corrected
  • Aortic and pulmonary valve velocity (mean, peak; mm/s)
  • Aortic and pulmonary valve pressure gradient (mean, peak; mmHg)
  • Aortic and pulmonary valve velocity time integral (VTI; cm)
  • Cardiac cycles
  • Mitral valve velocity (mm/s)
  • Mitral valve deceleration, isovolumetric relaxation time (IVRT), isovolumetric contraction time (IVCT; ms)

Figure_3I.csv

Source data for histological quantification associated with Figure 3. Aortic valve sections were stained with Alizarin Red staining and analyzed to quantify valve pathology following treatment. One-way ANOVA followed by post hoc Tukey HSD test; * p < 0.05. “ns” represents “not significant”.

Note: calcification was quantified as the percent calcified area (%), defined as the Alizarin Red-positive area divided by the total aortic valve area × 100. Therefore, it is reported as a percentage (%) rather than an absolute area (pixels).

Variables include:

• Experimental group (CXCR4fl/fl, EC CXCR4 KO+ PBS, EC CXCR4 KO+ NAMPT-Exo, or EC CXCR4 KO+ MSC-Exo)
• Biological replicate
• Area
• Quantitative histological measurements:
  • Calcified area
  • (%) Calcified

Figure_5.csv

This file contains data demonstrating the effects of treatment on endothelial-to-mesenchymal transition (EndMT). Endothelial cells were analyzed for endothelial and mesenchymal markers. Quantification of fluorescence intensity for α-SMA. Data are presented as mean ± SD. One-way ANOVA followed by post hoc Tukey HSD test; ** p < 0.01, *** p < 0.001. α-SMA indicates α-smooth muscle actin; and AU, arbitrary unit.

Variables include:

• Experimental group (CXCR4fl/fl, EC†CXCR4 KO, or EC CXCR4 KO+ NAMPT-Exo)
• Relative α-SMA expression

Figure_6.csv

This file contains quantitative microRNA expression data. Cardiac endothelial cells isolated from experimental mice (CXCR4fl/fl vs. EC CXCR4 KO group vs NAMPT-Exo treatment group) were analyzed using quantitative RT-PCR to determine microRNA expression.  Data are mean ± SD. One-way ANOVA followed by post hoc Tukey test; * p < 0.05, ** p < 0.01, and *** p <0.001. “ns” represents “not significant”. Expression values were calculated using the 2^-ΔΔCt method with U6 serving as the endogenous reference control.

Variables include:

• Experimental group (CXCR4fl/fl vs. EC CXCR4 KO group vs NAMPT-Exo)
• Biological replicate
• Relative miRNA expression
• miR-146a-3p
• miR-146a-5p
• miR-142a-5p
• miR-125b-5p
• U6 (Endog. Control)

Figure_7(A-B).csv

Source data demonstrating inhibition of EndMT in cultured endothelial cells.**** Primary mouse endothelial cells were treated with TGF-β1 in the presence or absence of a miR-146a-3p inhibitor. Quantification of fluorescence intensity for CD31 and α-SMA.

Variables include:

• Experimental group
• Relative CD31 expression
• Relative α-SMA expression
• Immunofluorescence intensity

Figure_7(C-D).csv

Source data for Western blot quantification associated with Figure 7.**** Protein expression following TGF-β1 stimulation and miR-146a-3p inhibition was quantified in different groups; expression was normalized to GAPDH. Data are mean ± SD. One-way ANOVA followed by post hoc Tukey HSD test; * p < 0.05,** p < 0.01, *** p < 0.001. “ns” represents “not significant”. TGF-β1 indicates transforming growth factor β1; CD31, cluster of differentiation 31, and alpha-SMA, alpha-smooth muscle actin.

Variables include:

• Experimental group
• Relative protein expression
• CD31
• VE-cadherin
• α-SMA
• Vimentin
• GAPDH normalization values

Abbreviations

AS – Aortic stenosis

EC – Endothelial cell

EndMT – Endothelial-to-mesenchymal transition

MSC – Mesenchymal stem cell

NAMPT – Nicotinamide phosphoribosyltransferase

NAMPT-Exo – NAMPT-enriched mesenchymal stem cell-derived exosomes

MSC-Exo – Exosomes derived from unmodified mesenchymal stem cells

CXCR4 – C-X-C chemokine receptor type 4

EF – Ejection fraction

FS – Fractional shortening

LVIDd – Left ventricular internal diameter during diastole

LVIDs – Left ventricular internal diameter during systole

α-SMA – Alpha-smooth muscle actin

PBS – Phosphate-buffered saline

TGF-β1 – Transforming growth factor beta 1

RT-qPCR – Quantitative reverse transcription polymerase chain reaction

VE-Cadherin   Vascular endothelial cadherin (endothelial marker)

The data were converted from Microsoft Excel to CSV format as requested by Dryad. Spreadsheet formatting, formulas, hyperlinks, comments, filters, and other Excel-specific features were removed during conversion. The CSV files contain the displayed values only.