Data from: Zeolitic imidazolate frameworks (ZIF-8) as a carrier in a topical ocular delivery system for the treatment of ocular diseases
Data files
Apr 07, 2026 version files 946.08 KB
-
README.md
3.04 KB
-
S10-ovaZIF-8_release_60_day.csv
3.29 KB
-
S11._BCOP_scoring_data.csv
3.44 KB
-
S11._HETCAM_scoring_data.csv
1.18 KB
-
S4-PXRD_data_blank.csv
104.62 KB
-
S4-PXRD_data.csv
104.97 KB
-
S5-FTIR_data_blank.csv
337.21 KB
-
S5-FTIR_data.csv
378.79 KB
-
S6-ovaZIF-8.txt
1.67 KB
-
S7-ZIF8-8.txt
2.09 KB
-
S8-ovaZIF-8_loading_plate_values.csv
524 B
-
S8-ovaZIF-8_loading.csv
2.04 KB
-
S9-ovaZIF-8_7_day_release.csv
3.21 KB
Abstract
Current systems for topical ocular drug delivery enable non-invasive administration of therapeutics to the eye but suffer from poor permeability and bioavailability, limiting therapeutic efficacy. Metal-organic frameworks are porous, crystalline materials that can serve as drug delivery systems and may improve these shortcomings. In this study, we explore the use of ZIF-8 as a representative metal-organic framework for topical ocular drug delivery. ZIF-8 particles were prepared via a simplified aqueous synthetic method and loaded with ovalbumin, a model molecule to simulate anti-angiogenic proteins that are used to treat retinal diseases. The physical properties of unloaded and loaded ZIF-8 were evaluated with a variety of spectroscopic, imaging, and desorption techniques that confirmed successful loading with ovalbumin (>46% loading capacity) without severe disruption to the structure of ZIF-8. In a bulk solution, loaded ovalbumin is gradually released in a linear fashion for up to 60 days. Loaded ZIF-8 applied to chicken eggs or bovine eyes showed minimal to no levels of irritation, supporting our hypothesis that ZIF-8 can be safely applied to ocular tissue. When applied to ex vivo bovine eyes, loaded agents administered in ZIF-8 penetrate the sclera, suggesting ZIF-8 may enhance permeability to intraocular tissue. These results highlight the potential of ZIF-8 as a carrier in topical ocular delivery systems and provide a crucial foundation to evaluate its efficacy in topical ocular drug delivery using in vivo models.
Dataset DOI: 10.5061/dryad.rv15dv4p6
Description of the data and file structure
This study highlights the potential of ZIF-8 as a carrier in topical ocular delivery systems and provides a crucial foundation to evaluate its efficacy in topical ocular drug delivery using in vivo models.
Files and variables
File: S4-PXRD_data_blank.csv
Description: Powder X-ray diffration (PXRD) data for unloaded ZIF-8. X=2 theta (degrees); Y=intensity (AU)
File: S4-PXRD_data.csv
Description: Powder X-ray diffraction (PXRD) data for ovalbumin-loaded ZIF-8. X=2 theta (degrees); Y=intensity (AU)
File: S5-FTIR_data_blank.csv
Description: Fourier transform infrared (FTIR) spectroscopy for unloaded ZIF showing wavelength and transmittance. Data are also shown adjusted for absorbance at baseline.
File: S5-FTIR_data.csv
Description: Fourier transform infrared (FTIR) spectroscopy for ovalbumin-loaded ZIF showing wavelength and transmittance. Data are also shown adjusted for absorbance at baseline.
File: S6-ovaZIF-8.txt
Description: Nitrogen isotherm for ovalbumin-loaded ZIF. Column 1 shows relative pressure (P/P0) and column 2 shows absorbed volume (cm3/g).
File: S7-ZIF8-8.txt
Description: Nitrogen isotherm for unloaded ZIF. Column 1 shows relative pressure (P/P0) and column 2 shows absorbed volume (cm3/g).
File: S8-ovaZIF-8_loading_plate_values.csv
Description: Optical density values by plate location and temperature.
File: S8-ovaZIF-8_loading.csv
Description: Optical density values by plate location and temperature; standard values below that. Loading capacity and encapsulation efficiency are below standards.
File: S9-ovaZIF-8_7_day_release.csv
Description: Ovalbumin release data over 7 days. Optical density values shown by microplate location. Standards and calculated total protein release below that.
File: S10-ovaZIF-8_release_60_day.csv
Description: Ovalbumin release data over 60 days. Optical density values shown by microplate location. Standards and calculated total protein release below that.
File: S11._BCOP_scoring_data.csv
Description: Bovine corneal opacity and permeability (BCOP) scoring
Opacity: cloudiness of the cornea; Epithelial Integrity: surface damage; Epithelial Detachment: peeling/loss of epithelium; Sum = total injury score per cornea
Higher = more severe damage
Treatment groups
NaOH: strong irritant (positive control); BZ (benzalkonium chloride): known irritant; Saline: negative control (should show minimal damage); OVA@ZIF: test; Blank ZIF: control
File: S11._HETCAM_scoring_data.csv
Description: Hen's egg test on chorioallantoic membrane (HETCAM) scoring. evaluates irritation potential by observing damage to blood vessels in a fertilized egg membrane.