Data from: Fate of Helicoverpa zea (Lepidoptera: Noctuidae) larvae exposed to low concentrations of Chlorantraniliprole
Data files
Jul 02, 2026 version files 91.01 KB
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Natchez_7_day.xlsx
9.44 KB
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Natchez_development.xlsx
63.72 KB
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Natchez_fecundity.xlsx
14.39 KB
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README.md
3.46 KB
Abstract
In recent years, chlorantraniliprole has been one of the most-used active ingredients for the control of lepidopteran pests, including the corn earworm, Helicoverpa zea (Boddie), on row crops in the southern U.S. Chlorantraniliprole is in the diamide class of insecticides. It possesses a high efficacy and comparatively long residual activity for the control of susceptible caterpillar species. These desirable insecticidal properties have led to an increase in its usage since its introduction in the U.S. Several studies have examined the susceptibilities of H. zea to chlorantraniliprole. These studies generally test neonate or third-instar caterpillars using insecticide-diet-incorporated bioassays and present lethal concentration (eg LC50) values based on survival at a particular time point. These assays provide partial information on the survival of H. zea for only a fragmented part of their development. In the current study, we examined the ultimate survival of the F1 generation of an H. zea colony originally collected from crimson clover (Trifolium incarnatum L., Fabaceae). Neonate larvae were placed on an artificial diet incorporating 6 different concentrations (0-16 ng/ml) of chlorantraniliprole and allowed to grow inside cups filled with the treated diet. Surviving male and female moths were paired within each treatment concentration, and the number of eggs laid per female was recorded. Survival to the pupal stage ranged from ~12 to 85%, while survival from pupae to moths ranged from 60 to 90%. As insecticide concentration increased, the days to reach the pupal stage increased, and pupal weight decreased. Once moths reached the pupal stage, days to emergence were similar for all concentrations. The number of eggs laid per adult female was not significantly related to the insecticide and averaged 45 to 120 eggs. These data present the fate of H. zea larvae that develop on chlorantraniliprole for their entire larval development and may be used to supplement traditional insecticide bioassays.
Dataset DOI: 10.5061/dryad.sn02v6xjn
Description of the data and file structure
This dataset contains survival and fitness measurements from an experiment investigating the response of neonate Helicoverpa zea larvae to low concentrations of chlorantraniliprole incorporated into an artificial diet. Larvae were held on insecticide treated diet for the duration of development. The data includes numbers of individuals surviving to the pupal stage and adult stages of development, the number of days to reach the pupal and adult stages, the sex and weight of pupae, the fecundity of female moths paired with males surviving the same concentrations of chlorantraniliprole. The dataset also includes the estimated lethal concentrations of neonate larvae seven days after exposure to chlorantraniliprole in a diet-incorporated bioassay.
Files and variables
File: Natchez_7_day.xlsx
Description: This file provides data for a seven-day bioassay using neonate Helicoverpa zea larvae exposed to 8 concentrations of chlorantraniliprole.
Variables
- Species: Helicoverpa zea
- Colony: the abbreviated name of the colony used in the study
- Dose: the concentration of chlorantraniliprole incorporated in artificial diet (ng/mL)
- no.: the number of individuals tested for that dose and replication
- response: the number of individuals that died or failed to molt within a seven-day period
File: Natchez_development.xlsx
Description: This file contains data related to the development of Helicoverpa zea larvae reared on low concentrations of chlorantraniliprole.
Variables
- Rep: The replication number of the experiment
- Trt: the concentration of chlorantraniliprole incorporated in artificial diet (ng/mL)
- Cup no: the identifier of each insect within that rep and treatment
- Diet date: date neonate larvae were placed on diet
- Pupal date: date that larvae reached the pupal stage of development (“X” indicates that an individual died during larval stage of development) (“missing” indicates that larvae escaped as neonates”
- Weight (mg): weight of pupae
- Sex: the sex of insects determined at the pupal stage of development
- Note: individuals noted as deformed and the sex of a pupa was not determined
- Moth Date: date a pupa successfully emerged as an adult
- "." indicate missing values
File: Natchez_fecundity.xlsx
Description: This file contains data related to the fecundity of single pairs (one male and one female) Helicoverpa zea moth that developed on low doses of chlorantraniliprole (moths within the same treatments were paired together)
Variables
- Cup no: the numbered single-paired mating cups (one male and female moth)
- Date in cup: the date moths were placed in rearing container
- Rep: the replication that moths were obtained from
- Trt: the concentration of chlorantraniliprole incorporated in artificial diet that moths developed on (ng/mL)
- F no.: the number of the cup where the female moth was obtained (from the Natchez development.xlsx spreadsheet)
- M no.: the number of the cup where the male moth was obtained (from the Natchez development.xlsx spreadsheet)
- Total eggs
Code/software
Any program that will open a spreadsheet, such as Excel is recommended.