Data for: A diminutive Tyrannosaur lived alongside Tyrannosaurus rex
Data files
Dec 02, 2025 version files 69.84 GB
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Alligator_mississippiensis.zip
8.66 GB
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Allosaurus_fragilis.zip
8.75 GB
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Allosaurus_jimmadseni.zip
2.09 GB
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Coelophysis_bauri.zip
1.56 GB
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Edmontosaurus_sp.zip
1.09 GB
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Eutyrannosaurians.zip
40.51 GB
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Nanotyrannus_lancensis.zip
4.67 GB
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Paleosuchus_palpebrosus.zip
911.12 MB
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README.md
2.74 KB
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Struthio_camelus.zip
1.60 GB
Abstract
Whether Nanotyrannus lancensis, a putative species of small tyrannosaurid, represents a distinct taxon or an immature Tyrannosaurus rex is a decades-long controversy. The N. lancesis holotype is an isolated skull and ceratobranchials, but limb osteohistology of Nanotyrannus-like individuals implies that these individuals were immature Tyrannosaurus, suggesting that the Nanotyrannus holotype is also immature. We demonstrate for the first time that ceratobranchial (‘hyoid’) histology is useful for ontogenetic assessment in extant and extinct archosaurs. The ceratobranchial histology of the N. lancensis holotype indicates that it was nearing or had reached skeletal maturity, suggesting that it is taxonomically distinct from the coeval Tyrannosaurus rex and that Hell Creek (and equivalent) ecosystems supported a diverse assemblage of predatory dinosaurs approaching the K-Pg extinction event.
https://doi.org/10.5061/dryad.sqv9s4ndn
Description of the data and file structure
The filenames of the associated whole-slide histological images reflect the taxon, specimen number, element, sampling location (if applicable), light regime, and magnification; "Genus_species_Museum####_Element_SamplingLocation_LightAbbreviation_Magnification.tif"
'Hyoid' as an element refers to ceratobranchial I.
Light regime abbreviations:
PP - Plane-polarized light
XP - Cross-polarized light
XPR - Cross-polarized light with a lambda retarder
R - Reflected light
OSEM - Optical shadow effect mode (a feature in the Keyence VHX-7100 digital microscope)
Light - in addition to one of the above light regimes, this scan included either increasing brightness either digitally or by wetting the slide
Example: Struthio_camelus_YPM112434_rightHyoid_cranialsample_XPR_200x.tif
Ostrich (Struthio camelus), specimen YPM 112434, right hyoid (ceratobranchial I), the cranial sampling location, cross-polarized light with a lambda retarder, 200x magnification
All filenames that include "Wagner" are images of slides made via automated grinder by Wagner Petrographics, Inc. All other slides were made by hand.
Files and variables
File: Alligator_mississippiensis.zip
Description: Whole-slide images of Alligator mississippiensis bone histology
File: Struthio_camelus.zip
Description: Whole-slide images of Struthio camelus bone histology
File: Allosaurus_fragilis.zip
Description: Whole-slide images of Allosaurus fragilis bone histology
File: Allosaurus_jimmadseni.zip
Description: Whole-slide images of Allosaurus jimmadseni bone histology
File: Coelophysis_bauri.zip
Description: Whole-slide images of Coelophysis bauri bone histology
File: Edmontosaurus_sp.zip
Description: Whole-slide images of Edmontosaurus sp. bone histology
File: Paleosuchus_palpebrosus.zip
Description: Whole-slide images of Paleosuchus palpebrosus bone histology
File: Eutyrannosaurians.zip
Description: Whole-slide images of Tyrannosaurus rex (LACM specimens) and an indeterminate eutyrannosaur (SWAU individual)
File: Nanotyrannus_lancensis.zip
Description: Whole-slide images of Nanotyrannus lancensis bone histology. One whole slide in the "Caudal" subfolder, with "CGMtraced" in the file name, contains a whole slide image with a cortical growth mark (CGM) traced around the cortex, as in Fig. S1. The whole-slide version of this figure permits others to trace this CGM on the large, high-resolution image.
