Data from: Phosphorus limitation does not drive loss of bony lateral plates in freshwater stickleback (Gasterosteus aculeatus)
Data files
Jul 13, 2020 version files 446.81 KB
Abstract
These datasets were used to assess whether a reduction in dietary phosphorus causes a growth trade-off with bony lateral plate development (via the Ectodysplasin locus) in marine threespine stickleback reared in freshwater conditions.
Wild marine stickleback fish carrying freshwater alleles at Ectodysplasin (Eda) were used to make F2 stickleback families which were each split equally among four experimental conditions: high-phosphorus diet + saltwater, high-phosphorus diet + freshwater, low-phosphorus diet + saltwater, low-phosphorus diet + freshwater.
F2 fish were grown to a pre-bony plate developmental stage (Timepoint 1), and two different during-bony plate developmental stages (Timepoints 2 & 3).
F2 fish were genotyped at Eda, sex was determined using molecular markers, and growth was assessed using standard length and weight. For fish collected at Timepoint 3, additional measurements were taken: count of bony lateral plates, % phosphorus content of bone, intestine length/weight, and phosphorus excretion rate.
Please see accompanying manuscript and README.txt file for further information on data collection and sample information.
See README.txt file for explanations of variables and data processing.