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Spatial reconstruction of the early hepatic transcriptomic landscape after an acetaminophen overdose using single-cell RNA sequencing

Umbaugh, David1; Ramachandran, Anup1; Jaeschke, Hartmut1

Published Aug 02, 2021; Updated Feb 28, 2022 on Dryad. https://doi.org/10.5061/dryad.t76hdr806

Data files

Aug 02, 2021 version files 6.89 MB
Feb 28, 2022 version files 41.39 MB

Abstract

We leveraged single-cell RNA sequencing to understand the early molecular events that define the hepatocyte response to acetaminophen exposure at a subpopulation level. We spatially assigned hepatocytes along the portol-central vein axis by using established landmark genes. By spatially assigning the hepatocytes were were able to account for innate differences in gene expression that existed along this gradient. The excel files herein provide the full list of differentially expressed genes between key subpopulations of interest. Additionally, we classified genes as either pericentral zonated, periportal zonated, or non-zonated, the full list of genes and their spatial assignments are included in the appropriate excel file.