Skip to main content
Dryad

An Omni-Mesoscope for multiscale high-throughput quantitative phase imaging of cellular dynamics and high-content molecular characterization

Ma, Hongqiang1; Chen, Maomao2; Xu, Jianquan2; Yang, Yaxin1; Zhao, Yongxin3; Liu, Yang1

Published Sep 24, 2024 on Dryad. https://doi.org/10.5061/dryad.t76hdr88t

Data files

Sep 24, 2024 version files 57.37 GB

Click names to download individual files Select up to 11 GB of files for zip download

Abstract

The mesoscope has emerged as a powerful imaging tool in biomedical research, yet its high cost and low resolution have limited its broader application. Here, we introduce the Omni-Mesoscope, a high-spatial-temporal and multimodal mesoscopic imaging platform built from cost-efficient off-the-shelf components. This system uniquely merges the capabilities of label-free quantitative phase microscopy to capture live-cell morpho-dynamics across thousands of cells with highly multiplexed fluorescence imaging for comprehensive molecular characterization. This Omni-Mesoscope offers a mesoscale field of view of approximately 5 mm² with a high spatial resolution down to 700 nm, enabling the capture of detailed sub-cellular features. We demonstrate its capability in delineating molecular characteristics underlying rare morpho-dynamic cellular phenomena, including cancer cell responses to chemotherapy and the emergence of polyploidy in drug-resistant cells. We also integrate expansion technique to enhance 3D volumetric super-resolution imaging of thicker tissues, opening new avenues for biological exploration at unprecedented scales and resolutions.