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Coalescent species’ delimitation in mimetic beetles of the genus Ceroglossus Solier (Coleoptera: Carabidae): The importance of species’ delineation to the understanding of the drivers of phenotypic diversity

Muñoz Ramírez, Carlos Patricio1; Knowles, Lacey2

Research facility: Agencia Nacional de Investigación y Desarrollo
Published May 08, 2026 on Dryad. https://doi.org/10.5061/dryad.t76hdr8c2

Data files

May 08, 2026 version files 9.60 GB

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Abstract

This data contains the raw genomic data generated via RADSeq from beetles of the genus Ceroglossus from south Chile. The data also include files used in the phylogenetic and species delimitation analyses conducted on these beetles.

The raw genomic data was generated from DNA extractions from 96 individuals belonging to 29 subspecies/color morphs and three species groups: C. chilensis, C. buqueti, and the C. darwini. Specimens were collected in South Chile and are preserved in 95% ethanol at the Museum of Zoology, University of Michigan (UMMZ).

DNA was extracted from the legs (usually one leg per individual) using a DNeasy Blood and Tissue Kit (Cat. No. 69581; Qiagen Inc.) following the manufacturer’s protocol.

DNA was double-digested with EcoRI and MseI restriction enzymes, followed by ligation of Illumina adaptor sequences and unique 10-base-pair barcodes. Ligation products were pooled among samples and size-selected using a Pippin Prep (Sage Science) machine (fragments of 180-280 bp), amplified by iProofTM High-Fidelity DNA Polymerase (BIO-RAD) with 12 cycles. The libraries were sequenced at The Centre for Applied Genomics (Hospital for Sick Children, Toronto, Canada) on the Illumina HiSeq2500 platform to generate 100-bp, single-end reads. To increase the data for some low-coverage samples, a second library was sequenced by selecting fragments of 350-450 bp.

More than 241 million reads were produced from the two lanes of Illumina sequencing (124 million and 116 million reads for the first and second libraries, respectively), which were subsequently pooled and processed using iPyrad to obtain files ready for phylogenetic analyses.