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Serial block face scanning electron micrographs of mouse outer retina

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Feb 12, 2025 version files 5.05 GB

Abstract

Serial block face images of outer mouse retina were obtained using an Apreo VS scanning electron microscope (Thermo Scientific). Isolated adult C57Bl6J mouse retina was fixed by immersion with standard EM fixative (2% Glutaraldehyde, 2% Paraformaldehyde, 0.1M Phosphate Buffer). Thomas Bargar, University of Nebraska Medical Center (Omaha, NE) Electron Microscopy Core Facility (EMCF), sectioned a volume of outer retina spanning 14.3 × 20.6 × 24 mm. Voxel dimensions are 6 × 6 × 40 nm and each image consisted of 3435 × 4000 pixels.