Serial block face scanning electron micrographs of mouse outer retina
Data files
Feb 12, 2025 version files 5.05 GB
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README.md
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Abstract
Serial block face images of outer mouse retina were obtained using an Apreo VS scanning electron microscope (Thermo Scientific). Isolated adult C57Bl6J mouse retina was fixed by immersion with standard EM fixative (2% Glutaraldehyde, 2% Paraformaldehyde, 0.1M Phosphate Buffer). Thomas Bargar, University of Nebraska Medical Center (Omaha, NE) Electron Microscopy Core Facility (EMCF), sectioned a volume of outer retina spanning 14.3 × 20.6 × 24 mm. Voxel dimensions are 6 × 6 × 40 nm and each image consisted of 3435 × 4000 pixels.
https://doi.org/10.5061/dryad.tb2rbp0cq
Description of the data and file structure
Serial block face images of outer mouse retina were obtained using an Apreo VS scanning electron microscope (Thermo Scientific). Isolated adult C57Bl6J mouse retina was fixed by immersion with standard EM fixative (2% Glutaraldehyde, 2% Paraformaldehyde, 0.1M Phosphate Buffer). Thomas Bargar, University of Nebraska Medical Center (Omaha, NE) Electron Microscopy Core Facility (EMCF), sectioned a volume of outer retina spanning 14.3 × 20.6 × 24 mm. Voxel dimensions are 6 × 6 × 40 nm and each image consisted of 3435 × 4000 pixels.
357 TIF files. ~14MB apiece.
Code/software
These files can be opened with any imaging program that accepts TIF files.
For three dimensional reconstructions of cells, we have used Amira software (RRID:SCR_007353).
Access information
Other publicly accessible locations of the data:
- Synapse Data Repository (https://www.synapse.org/Synapse:syn64720975).
Data was derived from the following sources:
- n/a