Data from: The embryonic DPPA3 gene stimulates the expression of pregnancy-related genes in bovine endometrial cells
Data files
May 02, 2025 version files 3.99 MB
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DEGsRNASeqDPPA3transfectedVScontrol.csv
19.66 KB
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EVsTransfectedWithGFP_inEndoCells.png
3.96 MB
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Primers_transcript_validation.csv
912 B
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README.md
2.69 KB
Abstract
This dataset contains supplementary materials associated with the study investigating extracellular vesicle (EV) mRNA transfer from bovine embryos to endometrial cells. The dataset includes (1) a table of primers used for quantitative PCR (qPCR), specifying primer sequences and target genes; (2) an image demonstrating the uptake of GFP mRNA by bovine endometrial epithelial cells following EV supplementation, confirming successful mRNA translation; and (3) a table of differentially expressed genes from RNA sequencing analysis, detailing gene names, log fold changes (logFC), p-values, and functional annotations. This dataset provides valuable resources for researchers studying EV-mediated communication, embryo-maternal interactions, and gene expression changes in response to EV-derived mRNA. No ethical or legal restrictions apply to the dataset, and it can be freely used for comparative analyses, validation studies, or further exploration of transcriptomic changes in reproductive biology.
Dataset DOI: 10.5061/dryad.tht76hf98
Description of the data and file structure
This dataset was generated as part of a study investigating the role of extracellular vesicle (EV)-derived mRNA in embryo-maternal communication in cattle. The experimental efforts focused on identifying full-length mRNA transcripts within EVs secreted by bovine embryos and assessing their impact on gene expression in maternal endometrial epithelial cells.
To achieve this, in vitro fertilized bovine embryos were cultured, and EVs were isolated from the culture media. RNA sequencing was performed to identify mRNA transcripts within these EVs. A key transcript, DPPA3, was selected for further functional analysis. Endometrial epithelial cells were supplemented with either embryo-derived EVs or in vitro transcribed DPPA3 mRNA to evaluate gene expression changes via RNA sequencing and proteomic analysis.
This dataset includes:
- A table of primers used for qPCR validation of gene expression changes.
- An image showing GFP mRNA uptake by bovine endometrial epithelial cells following supplementation of GFP transfected EVs
- A table of differentially expressed genes identified from RNA sequencing of cells transfected with DPPA3 mRNA, including gene names, log fold change (logFC), p-values, and functional annotations.
This dataset supports research on EV-mediated signaling, reproductive biology, and transcriptomic changes in embryo-maternal interactions.
Files and variables
File: Primers_transcript _validation.csv
Description: Primers used for transcript validation
File: DEGsRNASeqDPPA3transfectedVScontrol.csv
Description: Differential expression analysis of RNA-Seq from control vs DPPA3 transfected cells.
File: EVsTransfectedWithGFP_inEndoCells.png
Description: IVF derived EVs were transfected with GFP mRNA and subsequently supplemented to bovine endometrial epithelial cells. Cells were imaged 24 hours after EV supplementation to ensure successful uptake of the mRNA and translation into green fluorescent protein.
Code/software
n/a
Access information
Other publicly accessible locations of the data:
- RNA sequencing data has been deposited in National Center for Biotechnology Information (NCBI)’s Gene Expression Omnibus (GEO) and is accessible through GEO Series accession number GSE269049.
- All LC-MS/MS RAW files and results can be found at the MassIVE database (https://massive.ucsd.edu) under the following accession MassIVE 000096795.