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Imaging data for: Arp2/3 involvement in intercellular bridge membrane fluctuations and constriction during neural stem cell divisions

LaFoya, Bryce1; Prehoda, Kenneth2

Published Mar 14, 2026 on Dryad. https://doi.org/10.5061/dryad.tmpg4f5d1

Data files

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Abstract

This dataset contains imaging data of Drosophila neural stem cells within larval brains, capturing cellular morphology and spatial organization. It accompanies the study “Arp2/3 involvement in intercellular bridge membrane fluctuations and constriction during neural stem cell divisions,” which investigates the mechanisms of intercellular bridge (ICB) remodeling during the final stages of neural stem cell division in the Drosophila larval brain. Unlike ICBs in cultured cells, which have flanking regions that thin as sibling cells move apart, neural stem cell ICBs lack flanking arms and constrict primarily at the midbody. Using super-resolution, full-volume live imaging, the study revealed dynamic plasma membrane fluctuations surrounding the ICB. Inhibition of the Arp2/3 complex, a key nucleator of branched actin filaments, reduced these fluctuations and impaired ICB thinning. These findings suggest that dynamic branched actin networks contribute to ICB membrane remodeling and help support constriction of the intercellular bridge during neural stem cell division. These data may be reused for image analysis, quantitative reanalysis, or methodological development. Users are expected to cite both this dataset and the associated publication in any use, presentation, or derivative work. While the data are publicly available, users are encouraged to contact the dataset authors for clarification, guidance, or collaboration.