Data and source code from: Contingency and selection in mitochondrial genome dynamics
Data files
May 20, 2022 version files 14.23 GB
-
grande_YPD_1.fastq
128.45 MB
-
grande_YPD_2.fastq
275.78 MB
-
grande_YPD_3.fastq
156.63 MB
-
grande_YPD_4.fastq
122.23 MB
-
grande_YPD_5.fastq
109.51 MB
-
grande_YPD_6.fastq
122.92 MB
-
grande_YPG_1.fastq
294.32 MB
-
grande_YPG_2.fastq
194.26 MB
-
grande_YPG_3.fastq
141.26 MB
-
grande_YPG_4.fastq
147.29 MB
-
inverted_duplication_read_locations_chrI-III.txt
10.28 KB
-
petite_family1a_1.fastq
745.27 MB
-
petite_family1a_2.fastq
296.50 MB
-
petite_family1a_3.fastq
561.37 MB
-
petite_family1b.fastq
234.30 MB
-
petite_family1c.fastq
365.83 MB
-
petite_family1d.fastq
258.58 MB
-
petite_family2_1.fastq
674.62 MB
-
petite_family2_2.fastq
631.96 MB
-
petite_family2_3.fastq
416.98 MB
-
petite_family2_4.fastq
312.53 MB
-
petite_family2_5.fastq
579.95 MB
-
petite_family3_1.fastq
256.90 MB
-
petite_family3_2.fastq
453.87 MB
-
petite_family3_3.fastq
420.33 MB
-
petite_family3_4.fastq
414.90 MB
-
petite_family3_5.fastq
311.12 MB
-
petite_family3_6.fastq
149.51 MB
-
petite_family3_7.fastq
413.14 MB
-
petite_family4a_1.fastq
446.63 MB
-
petite_family4a_2.fastq
428.69 MB
-
petite_family4a_3.fastq
503.27 MB
-
petite_family4b_1.fastq
235.03 MB
-
petite_family4b_2.fastq
414.90 MB
-
petite_family4b_3.fastq
333.70 MB
-
petite_family5_1.fastq
113.02 MB
-
petite_family5_2.fastq
146.77 MB
-
petite_family5_3.fastq
146.43 MB
-
petite_family6_1.fastq
305.27 MB
-
petite_family6_2.fastq
452.28 MB
-
petite_family6_3.fastq
256.81 MB
-
petite_family7_1.fastq
147.97 MB
-
petite_family7_2.fastq
223.47 MB
-
petite_family7_3.fastq
114.64 MB
-
petite_family8a_1.fastq
171.83 MB
-
petite_family8a_2.fastq
225.15 MB
-
petite_family8b.fastq
158.45 MB
-
petite_family9_1.fastq
107.41 MB
-
petite_family9_2.fastq
108.73 MB
-
README.txt
2.10 KB
Abstract
Eukaryotic cells contain numerous copies of mitochondrial DNA (mtDNA), allowing for the coexistence of mutant and wild-type mtDNA in individual cells. The fate of mutant mtDNA depends on their relative replicative fitness within cells and the resulting cellular fitness within populations of cells. Yet the dynamics of the generation of mutant mtDNA and features that inform their fitness remain unaddressed. Here we utilize long read single-molecule sequencing to track mtDNA mutational trajectories in Saccharomyces cerevisiae. We show a previously unseen pattern that constrains subsequent excision events in mtDNA fragmentation. We also provide evidence for the generation of rare and contentious non-periodic mtDNA structures that lead to persistent diversity within individual cells. Finally, we show that measurements of relative fitness of mtDNA fit a phenomenological model that highlights important biophysical parameters governing mtDNA fitness. Altogether, our study provides techniques and insights into the dynamics of large structural changes in genomes that may be applicable in more complex organisms.
Dataset was collected with an Oxford Nanopore MinION Mk1B following the experimental description in methods. Raw Nanopore reads were basecalled and demultiplexed with the ONT Guppy package 3.1.5-1.
See README file