Data from: Hybridization, reinforcement selection and sex-dependent reproductive character displacement of sperm and egg recognition proteins
Data files
May 02, 2025 version files 678.65 KB
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Dryad_Data_for_Evolution_2025_CSP_paper.xlsx
31.28 KB
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M_fran_bindin_alleles_for_haplotype.txt
80.22 KB
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M_fran_CUB1_alleles_for_haplotype.txt
140.55 KB
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M_fran_CUB7_alleles_for_haplotype.txt
130.03 KB
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M_fran_TSP13_alleles_for_haplotype.txt
108.32 KB
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M_fran_TSP8_alleles_for_haplotype.txt
165.61 KB
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README.md
6.80 KB
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S_droeb_CUB1_alleles_for_haplotype.TXT
7.28 KB
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S_droeb_CUB7_alleles_for_haplotype.txt
8.55 KB
Abstract
The establishment of reproductive isolation between species via gametic incompatibility initially requires within-species selection for variation in reproductive compatibility. We investigate how the generation of within-species variation in sperm and egg recognition proteins, potentially via sexual conflict, influences reproductive isolation between two partially sympatric sea urchin species; the North American west coast Mesocentrotus franciscanus and the circumpolar Strongylocentrotus droebachiensis. Barriers to hybridization are stronger when eggs are given a choice of conspecific versus heterospecific sperm and the variation in hybridization among crosses can be explained by whether the sperm or egg protein variant is ancestral or derived. Derived proteins can be recognized as different and prevent hybridization. Examination of the allele frequencies of these proteins in M. franciscanus in and out of sympatry with S. droebachiensis along the west coast of North America reveals evidence of reinforcement selection and reproductive character displacement in eggs but not sperm, which likely reflects the differential cost of hybridization for males and females.
Dataset DOI: 10.5061/dryad.vdncjsz5n
Description of the data and file structure
The data presented here are from a study examining how gamete recognition proteins influence the likelihood of hybrid fertilization between two sea urchin species; Mesocentrotus franciscanus and Strongylocentrotis droebachiensis. Laboratory experiments examined crosses in which eggs had a choice between the sperm from these two species or in no-choice trials. The probability of hybridization was examined as a function of the sperm and egg recognition genotype of the two parents. In addition to these experiments a geographic survey of the allele frequencies of these sperm and egg proteins was examined in M. franciscanus along the west coast of North America in areas in sympatry with S. droebachiensis and in allopatry.
The Excel file has all the data presented in the figures in the manuscript and what was used in the statistical analyses. Each sheet in the Excel file has the data for one figure with an explanation of the heading in each column. The text files have the sequence data, in fasta format, for all the M. franciscanus and S. droebachiensis alleles (and their frequencies) that comprised the haplotype networks presented in the paper's supplement.
Files and variables
File: M_fran_bindin_alleles_for_haplotype.txt
Description: text file in fasta format of the sperm bindin alleles used to generate the haplotype network in M. franciscanus
Variables
- base pair identities
File: M_fran_CUB7_alleles_for_haplotype.txt
Description: text file in fasta format of the EBR1 CUB7 alleles used to generate the haplotype network in M. franciscanus
Variables
- base pair identities
File: M_fran_TSP8_alleles_for_haplotype.txt
Description: text file in fasta format of the EBR1 TSP8 alleles used to generate the haplotype network in M. franciscanus
Variables
- base pair identities
File: M_fran_TSP13_alleles_for_haplotype.txt
Description: text file in fasta format of the EBR1 TSP13 alleles used to generate the haplotype network in M. franciscanus
Variables
- base pair identities
File: M_fran_CUB1_alleles_for_haplotype.txt
Description: text file in fasta format of the EBR1 CUB1 alleles used to generate the haplotype network in M. franciscanus
Variables
- Base pair identities
File: S_droeb_CUB1_alleles_for_haplotype.TXT
Description: text file in fasta format of the EBR1 CUB1 alleles used to generate the haplotype network in S. drobachiensis
Variables
base pair identities
File: S_droeb_CUB7_alleles_for_haplotype.txt
Description: text file in fasta format of the EBR1 CUB7 alleles used to generate the haplotype network in S. drobachiensis
Variables
- Base pair identities
File: Data_for_Evolution_2025_CSP_paper_author_name_removed.xlsx
Description: Excel file for all the figures and statistical analyses presented in the paper.
Fig 1 spreadsheet: This is the data used to generate Fig. 1 and evaluated in Supplemental Table 4. These are the results of laboratory crosses examining the proportion of eggs fertilized when eggs are not given a choice of sperm source. Column headings: “Species of eggs” is either S. droebachiensis or M. franciscanus; “Sperm concentration” is either low (sperm limited) or high (sperm saturated); “Type of cross” is either heterospecific (hybrid cross) or conspecific.
Fig 2 spreadsheet: This is the data used to generate Fig. 2 and test for the effects of sperm concentration and the ratio of S. droebachiensis/M. franciscanus sperm. There are the results of laboratory crosses where S. droebachiensis eggs were tested against a mixture of sperm from both species and the proportion of hybrid embyros produced. Column headings: “Proportion of S. droebachiensis sperm” is the proportion of the total sperm consisting of S. droebachiensis sperm; “Sperm concentration” is either saturating (sat) or limiting (lim); “Proportion of hybrid embryos” is the proportion of embryos genotyped as being sired by M. franciscanus sperm.
Fig 3 spreadsheet: This is the data used to generate Fig. 3 and the analysis presented in Supplemental Table 5. These are the results of laboratory crosses testing a 90%/10% mixture of M. franciscanus sperm/S. droebachieneis sperm on S. droebachiensis eggs as a function of protein variants in sperm and egg recognition proteins. The proportion of embryo produced by M. franciscanus sperm (hybrids) were assigned via PCR or species-specific primers. Column headings: “M. franciscanus bindin sites 16 and 38” is the genotype (amino acid code at those sites) of the sire at the sperm bindin locus;” S.droeb CUB1_17” is the genotype (amino acid code) of the dame at the CUB1 repeat in the EBR1 gene; S.droeb CUB7_44” is the genotype (amino acid code) of the dame at the CUB7 repeat in the EBR1 gene; “Proportion hybrid” is the proportion of hybrids produced in each cross.
Fig 4 spreadsheet: This is the data for Fig. 4 and analysis presented in Supplemental Table 6. These were laboratory crosses testing the proportion of *M. franciscanus *eggs fertilized by *S. droebachiensis *sperm as a function of sperm concentration and the genotype of the four variable EBR1 repeats in M. franciscanus females. Dots indicate missing data. Column headings: “Date” is the date of the experimental cross; “TSP8_31” is the genotype (amino acid code) of the dame at the TSP8 repeat in the EBR1 gene; “TSP13_27” is the genotype (amino acid code) of the dame at the TSP13 repeat in the EBR1 gene; CUB1_27” is the genotype (amino acid code) of the dame at the CUB1 repeat in the EBR1 gene; “CUB7_51 is the genotype (amino acid code) of the dame at the TCUB7 repeat in the EBR1 gene; “droe sperm” is the sperm concentration (sperm/mL); “Prop” is the proportion of eggs fertilized in that cross.
Fig5 spreadsheet: This is the data for Fig. 5 and analysis presented in Supplemental Table 7. These data are the allele frequencies of the four variable EBR1 repeats and sperm bindin gene in M. franciscanus at sites in (north) and out (south) of sympatry with S. droebachiensis. Column headings: “site” name of site; “Lat” is the latitude of the site; following 11 columns are the allele frequencies for each repeat; 14th column is whether the site is north or south of Point Conception, CA, USA; Last column is the average test diameter of the sea urchin in the sample.
These data are the results of fertilization crosses between the sea urchins, Mesocentrotus franciscanus and Strongylocentrotus droebachiensis. Tests were both choice (sperm from both males together) and no choice experiments. Data were analyzed as a function of the sire's sperm bindin genotype and the dame's EBR1 genotype. Genotyping was conducted using PCR and Sangar sequencing. Patterns of allele frequencies for these loci were investigated over the species range for M. franciscanus to investigate evidence of reproctive character displacement. These data files contain information of the genotypes of the parents in the cross and the fraction of eggs fertilized by conspecific or heterospecific sperm. Fasta files of the haplotypes found for each locus are also presented.