Dataset DOI: 10.5061/dryad.vdncjszbc

Description of the data and file structure

This dataset includes fiber photometry, single-unit optrode recordings, optogenetics, simultaneous cystometry and electromyography (EMG), anatomical tracing data, and time‑series analysis from mouse models, organized in separate Excel sheets that follow the figure order of the article, with individual trial records and group statistics. For a complete account of experimental protocols, please consult the main article.

Files and variables

File: Fig1_data.xlsx

Description: This dataset contains source data from a study investigating the activity of estrogen receptor 1‑positive neurons in the pontine micturition center (PMC^ESR1+) and their correlations with bladder contraction and external urethral sphincter (EUS) relaxation during voiding in ESR1-CRE mice. Sheet "Panel C" contains the baseline‑subtracted firing rates before and during voiding for PMC^ESR1+ and non‑PMC^ESR1+ cells (i.e., neurons that do not express ESR1). Sheet "Panel G" contains the data for the correlation coefficients between calcium (Ca²⁺) signals (expressed as ΔF/F, % change from baseline) and bladder contraction amplitude (in cm H₂O), along with the corresponding shuffled data. Sheet "Panel H" contains the data for the correlation coefficients between calcium (Ca²⁺) signals (expressed as ΔF/F, % change from baseline) and external urethral sphincter electromyography (EUS‑EMG) bursting events, along with the corresponding shuffled data.

Variables

• Estrogen receptor 1‑positive neurons in the pontine micturition center (PMC^ESR1+ cells)
• Neurons that do not express estrogen receptor 1 in the pontine micturition center (non‑PMC^ESR1+ cells)
• Voiding phase (before vs. during voiding)
• Baseline‑corrected average firing rate (Hz, spikes per second) 
• Correlation coefficient with bladder contraction events (Ca²⁺ signals and shuffled data) 
• Correlation coefficient with EUS-EMG bursting events (Ca²⁺ signals and shuffled data) 

File: Fig1_S1_data.xlsx

Description: This file presents the source data from a study investigating the activity of estrogen receptor 1‑positive neurons in the pontine micturition center (PMC^ESR1+) during voiding in ESR1‑CRE mice. Sheet "Panel E" contains the amplitude of voiding‑related calcium (Ca²⁺) signals (expressed as ΔF/F, % change from baseline) for three groups: (1) the genetically encoded calcium indicator GCaMP6f (GCaMP6f) experimental group, (2) the enhanced yellow fluorescent protein (EYFP) control group, and (3) the shuffled data. Sheet "Panel F" contains the percentage (%) of voiding‑related Ca²⁺ events (defined as transient ΔF/F events that occurred within the voiding phase) for the same three groups: GCaMP6f, EYFP, and shuffled.

Variables

• Genetically encoded calcium indicator GCaMP6f group (GCaMP6f)
• Enhanced yellow fluorescent protein group (EYFP)
• Random permutation control group (shuffled)
• Voiding-related Ca²⁺ signal amplitude (ΔF/F, % change from baseline)
• Percentage of voiding-related Ca²⁺ transients (in %)

File: Fig1_S3_data.xlsx

Description: This file presents the source data related to the identification of recorded estrogen receptor 1‑positive neurons in the pontine micturition center (PMC^ESR1+) during optrode recordings in ESR1‑CRE mice. Sheet "Panel C" contains correlation coefficients distribution between spontaneous and light‑induced spikes across all recorded PMC^ESR1+ units. Sheet "Panel D" contains the success rates (percentage of trials with a light‑evoked spike, in %) and temporal jitter (standard deviation of spike timing relative to light onset, in milliseconds, ms) for the first light‑induced spike in all recorded units. Sheet "Panel E" contains the latency distribution (time from light onset to the first evoked spike, in ms) for all recorded PMC^ESR1+ units.

Variables

• Estrogen receptor 1‑positive neurons in the pontine micturition center (PMC^ESR1+ units)
• Spike correlation coefficient (between spontaneous and light-induced spikes)
• Success rate of first light-evoked spike (in %)
• Temporal jitter of first light-evoked spike (in ms)
• Spike latency (in ms)

File: Fig1_S4_data.xlsx

Description: This file presents the source data related to non-voiding contractions (NVCs) and their relationship with calcium (Ca²⁺) signals in estrogen receptor 1‑positive neurons in the pontine micturition center (PMC^ESR1+) during simultaneous cystometry and urethral electromyography recording in urethane-anesthetized SR1‑CRE mice. Sheet "Panel D" contains the proportion (%) of Ca²⁺ transients that are temporally associated with bladder contraction events and external urethral sphincter electromyography (EUS‑EMG) bursting events. Sheet "Panel E" contains the peak bladder pressure (in cm H₂O) for non‑voiding contractions (NVCs) and voiding contractions (VCs). Sheet "Panel F" contains the peak Ca²⁺ signal amplitude (ΔF/F, % change from baseline) during NVCs and VCs, as well as the Ca²⁺ signal amplitude (ΔF/F, %) at EUS‑EMG bursting onset and offset during VCs.

Variables

• Proportion of Ca²⁺ transients associated with bladder contraction (in %)
• Proportion of Ca²⁺ transients associated with EUS-EMG bursting events (in %)
• Non‑voiding contractions (NVCs)
• Voiding contractions (VCs)
• Peak bladder pressure (in cm H₂O)
• Peak Ca²⁺ signals during NVCs and VCs (ΔF/F, % change from baseline)
• Ca²⁺ signal amplitude at EUS‑EMG bursting onset and offset during VCs (ΔF/F, %)

File: Fig2_data.xlsx

Description: This file presents the source data related to the effects of photoinhibition (60 s) on voiding parameters in the estrogen receptor 1‑positive neurons in the pontine micturition center expressing the light‑activated chloride channel GtACR1 (PMC^{ESR1‑GtACR1}) experimental group and the estrogen receptor 1‑positive neurons expressing the fluorescent control protein mCherry (PMC^{ESR1‑mCherry}) control group in SR1‑CRE mice. The parameters were measured in three phases: before, during, and after photoinhibition. Sheet "Panel C" contains the data for the void area (in cm²) before, during, and after photoinhibition. Sheet "Panel E" contains the data for voiding duration (in seconds) before, during, and after photoinhibition. Sheet "Panel H" contains the change in bladder pressure (Δpressure, in cm H₂O) during voiding across the three phases. Sheet "Panel I" contains the external urethral sphincter electromyography (EUS‑EMG) bursting duration (in seconds) during voiding across the three phases.

Variables

• Estrogen receptor 1‑positive neurons in the pontine micturition center expressing the light‑activated chloride channel GtACR1 group (PMC^ESR1-GtACR1)
• Estrogen receptor 1‑positive neurons expressing the fluorescent control protein mCherry group (PMC^ESR1-mCherry)
• Photoinhibition condition (before, during, after photoinhibition)
• Void area (in cm²)
• Voiding duration (in seconds)
• Change in bladder pressure during voiding (Δpressure, in cm H₂O)
• EUS-EMG bursting duration (in seconds)

File: Fig2_S1_data.xlsx

Description: This file presents the source data related to the effects of acute photoinhibition (60 s) of estrogen receptor 1‑positive neurons in the estrogen receptor 1‑positive neurons in the pontine micturition center expressing the light‑activated chloride channel GtACR1 (PMC^{ESR1‑GtACR1}) experimental group and the estrogen receptor 1‑positive neurons expressing the fluorescent control protein mCherry (PMC^{ESR1‑mCherry}) control group on bladder and external urethral sphincter (EUS) function in ESR1‑CRE mice. Sheet "Panel B" contains the latency (in seconds) from light onset to voiding suspension (i.e., cessation of urine flow) following acute photoinhibition. Sheet "Panel D" contains the latency (in seconds) from light onset to termination of external urethral sphincter electromyography (EUS‑EMG) bursting following acute photoinhibition. Sheet "Panel E" contains the bladder threshold pressure (in cm H₂O) during voiding across three phases – before ("Pre"), during ("On"), and after ("Post") photoinhibition for both the PMC^{ESR1‑GtACR1} (experimental, light‑inhibited) and PMC^{ESR1‑mCherry} (control) groups.

Variables

• Estrogen receptor 1‑positive neurons in the pontine micturition center expressing the light‑activated chloride channel GtACR1 group (PMC^ESR1-GtACR1)
• Estrogen receptor 1‑positive neurons expressing the fluorescent control protein mCherry group (PMC^ESR1-mCherry)
• Photoinhibition condition (before, during, after photoinhibition)
• Latency to voiding suspension after light onset (in seconds)
• Latency to EUS-EMG bursting termination after light onset (in seconds)
• Bladder threshold pressure during voiding (in cm H₂O)

File: Fig2_S2_data.xlsx

Description: This file presents the source data related to the effects of acute photoinhibition (5 s) of estrogen receptor 1‑positive neurons in the pontine micturition center expressing the light‑activated chloride channel GtACR1 (PMC^{ESR1‑GtACR1}) experimental group and the estrogen receptor 1‑positive neurons expressing the fluorescent control protein mCherry (PMC^{ESR1‑mCherry}) control group on voiding and bladder‑sphincter function in SR1‑CRE mice. Sheet "Panel C" contains the data for void area (in cm²) before, during, and after 5 s photoinhibition in PMC^ESR1-GtACR1 and PMC^ESR1-mCherry groups. Sheet "Panel E" contains the data for voiding duration (in seconds) before, during, and after 5 s photoinhibition in the PMC^ESR1-GtACR1 and PMC^ESR1-mCherry groups. Sheet "Panel F" contains the data for latency (in seconds) to voiding suspension after light onset. Sheet "Panels H‑K" contain data for the effects of 5 s photoinhibition on bladder pressure and external urethral sphincter electromyography (EUS‑EMG), including: change in bladder pressure during voiding (Δpressure, in cm H2O) in Panel H; bladder threshold pressure (in cm H2O) in Panel I; EUS‑EMG bursting area under the curve (AUC, in mV*s) in Panel J; and EUS‑EMG bursting duration (in seconds) in Panel K. Sheet "Panel L" contains the latency (in seconds) from light onset to termination of EUS‑EMG bursting following 5 s photoinhibition.

Variables

• Estrogen receptor 1‑positive neurons in the pontine micturition center expressing the light‑activated chloride channel GtACR1 group (PMC^ESR1-GtACR1)
• Estrogen receptor 1‑positive neurons expressing the fluorescent control protein mCherry group (PMC^ESR1-mCherry)
• Photoinhibition condition (before, during, and after 5-s photoinhibition)
• Void area (in cm²)
• Voiding duration (in seconds)
• Latency to voiding suspension after light onset (in seconds)
• Change in bladder pressure during voiding (Δpressure, in cm H₂O)
• Bladder threshold pressure (in cm H₂O)
• EUS‑EMG bursting area under the curve (EUS-EMG bursting AUC, in mV*s)
• EUS-EMG bursting duration (in seconds)
• Latency to EUS-EMG bursting termination after 5-s photoinhibition (in seconds)

File: Fig2_S3_data.xlsx

Description: This file presents the source data related to the effects of acute photoactivation (5 s) of estrogen receptor 1‑positive neurons in the pontine micturition center (PMC^ESR1+) on voiding and bladder‑sphincter function in ESR1‑CRE mice, comparing the PMC^ESR1‑ChR2 group (expressing Channelrhodopsin‑2, a light‑gated cation channel that depolarizes neurons upon blue light illumination; experimental, light‑activated) and the PMC^{ESR1‑mCherry} group (expressing the red fluorescent protein mCherry; control, no light‑sensitive protein). Sheet "Panel C" contains the percentage (%) of photoactivation‑associated voiding events (i.e., voids that occurred during or immediately after light stimulation) for both groups. Sheet "Panel D" contains the latency (in seconds) from light onset to voiding onset following acute photoactivation. Sheet "Panel G" contains the change in bladder pressure (Δpressure, in cm H₂O) and the bladder pressure ratio during photoactivation for both groups. Sheet "Panel H" contains the percentage (%) of photoactivation‑associated bladder contractions and external urethral sphincter electromyography (EUS‑EMG) bursting events during photoactivation for both groups.

Variables

• Estrogen receptor 1‑positive neurons in the pontine micturition center expressing Channelrhodopsin‑2 (ChR2) group (PMC^ESR1-ChR2)
• Estrogen receptor 1‑positive neurons expressing the fluorescent control protein mCherry group (PMC^ESR1-mCherry)
• Percentage of photoactivation-associated voiding events (in %)
• Latency to voiding onset after light onset (in seconds)
• Change in bladder pressure (Δpressure, in cm H₂O)
• Bladder pressure ratio
• Percentage of photoactivation-associated bladder contractions (in %)
• Percentage of photoactivation-associated EUS-EMG bursting (in %)

File: Fig2_S4_data.xlsx

Description: This file presents the source data related to the effects of regular‑interval photoactivation (5 s) of estrogen receptor 1‑positive neurons in the pontine micturition center (PMC^ESR1+) on bladder contraction and external urethral sphincter electromyography (EUS‑EMG) bursting during simultaneous cystometry and urethral electromyography recording in urethane-anesthetized SR1‑CRE mice. Data are presented for both the PMCESR1‑ChR2 (experimental, light‑activated) and PMCESR1‑mCherry (control) groups. Sheet "Panel D" contains the percentage (%) of bladder contractions and EUS‑EMG bursting events that occurred during the photoactivation periods for both groups. Sheet "Panel E" contains the change in bladder pressure (Δpressure, in cm H2O) and the bladder pressure ratio during photoactivation for both groups.

Variables

• Estrogen receptor 1‑positive neurons in the pontine micturition center expressing Channelrhodopsin‑2 (ChR2) group (PMC^ESR1-ChR2)
• Estrogen receptor 1‑positive neurons expressing the fluorescent control protein mCherry group (PMC^ESR1-mCherry)
• Percentage of photoactivation-associated bladder contractions (in %)
• Percentage of photoactivation-associated EUS-EMG bursting (in %)
• Change in bladder pressure (Δpressure, in cm H₂O)
• Bladder pressure ratio

File: Fig3_data.xlsx

Description: This file presents the source data related to the effects of pudendal nerve transection (PDNx), alone or in combination with pelvic nerve transection (PLNx), on bladder contraction and external urethral sphincter electromyography (EUS‑EMG) bursting during photoactivation of estrogen receptor 1‑positive neurons in the pontine micturition center (PMC^ESR1+) in urethane‑anesthetized ESR1‑CRE mice. Data were collected under three conditions: intact (before any transection), after pudendal nerve transection alone (PDNx), and after combined transection of both the pudendal and pelvic nerves (PDNx+PLNx). Sheet "Panel D" contains the change in bladder pressure (Δpressure, in cm H₂O) and the bladder pressure ratio during photoactivation for each condition. Sheet "Panel E" contains the percentage (%) of photoactivation‑associated bladder contractions and EUS‑EMG bursting events during photoactivation for each condition.

Variables

• No transection (intact)
• Pudendal nerve transection (PDNx)
• Combined pudendal and pelvic nerve transection (PDNx+PLNx)
• Change in bladder pressure (Δpressure, in cm H₂O)
• Bladder pressure ratio
• Percentage of photoactivation-associated bladder contractions (in %)
• Percentage of photoactivation-associated EUS-EMG bursting (in %)

File: Fig4_data.xlsx

Description: This file presents the source data related to the effects of initial pelvic nerve transection (PLNx), alone or in combination with subsequent pudendal nerve transection (PDNx), on bladder contraction and external urethral sphincter electromyography (EUS‑EMG) bursting during photoactivation of estrogen receptor 1‑positive neurons in the pontine micturition center (PMC^ESR1+) in urethane‑anesthetized ESR1‑CRE mice. Data were collected under three conditions: intact (before any transection), after pelvic nerve transection alone (PLNx), and after combined transection of both the pelvic and pudendal nerves (PLNx+PDNx). Sheet "Panel D" contains the change in bladder pressure (Δpressure, in cm H₂O), the bladder pressure ratio, and the percentage (%) of photoactivation‑associated bladder contractions during photoactivation for each condition. Sheet "Panel E" contains the EUS‑EMG bursting area under the curve (AUC, in mV·s), EUS‑EMG bursting duration (in seconds), and the percentage (%) of photoactivation‑associated EUS‑EMG bursting events during photoactivation for each condition.

Variables

• No transection (intact)
• Pelvic nerve transection (PLNx)
• Combined pelvic and pudendal nerve transection (PLNx+PDNx)
• Change in bladder pressure (Δpressure, in cm H₂O)
• Bladder pressure ratio
• EUS‑EMG bursting area under the curve (EUS-EMG bursting AUC, in mV*s)
• EUS-EMG bursting duration (in seconds)
• Percentage of photoactivation-associated bladder contractions (in %)
• Percentage of photoactivation-associated EUS-EMG bursting (in %)

File: Fig4_S1_data.xlsx

Description: This file presents the source data related to the effects of initial pelvic nerve transection (PLNx), alone or in combination with subsequent pudendal nerve transection (PDNx), on photoactivation-induced bladder contraction and external urethral sphincter electromyography (EUS‑EMG) bursting in the unfilled bladder in urethane‑anesthetized ESR1‑CRE mice. Photoactivation was applied to estrogen receptor 1‑positive neurons in the pontine micturition center (PMC^ESR1+). Data were collected under three conditions: intact (before any transection), after pelvic nerve transection alone (PLNx), and after combined transection of both the pelvic and pudendal nerves (PLNx+PDNx). Sheet "Panel D" contains the change in bladder pressure (Δpressure, in cm H₂O) and the bladder pressure ratio during photoactivation for each condition. Sheet "Panel E" contains the percentage (%) of photoactivation‑associated bladder contractions and photoactivation‑associated EUS‑EMG bursting events for each condition.

Variables

• No transection (intact)
• Pelvic nerve transection (PLNx)
• Combined pelvic and pudendal nerve transection (PLNx+PDNx)
• Change in bladder pressure (Δpressure, in cm H₂O)
• Bladder pressure ratio
• Percentage of photoactivation-associated bladder contractions (in %)
• Percentage of photoactivation-associated EUS-EMG bursting (in %)

File: Fig5_data.xlsx

Description: This file presents the source data related to the effects of photoactivation of estrogen receptor 1‑positive neurons in the pontine micturition center (PMC^ESR1+) on voiding‑related parameters before and after pelvic nerve transection (PLNx) in freely moving ESR1‑CRE mice. Data were collected from two groups: sham (control, no nerve transection) and pelvic nerve transection (PLNx). For each group, measurements were taken under two conditions: baseline (before any intervention) and post‑transection (after surgery for the PLNx group, or after sham surgery for the sham group). Sheet "Panel C" contains the percentage (%) of light‑induced voiding events in both groups. Sheet "Panel D" contains the light‑induced voiding area (in cm²) in both groups. Sheet "Panel E" contains the latency (in seconds) from light onset to voiding onset following light stimulation in both groups.

Variables

• Sham group (control, no nerve transection)
• Pelvic nerve transection group (PLNx)
• Baseline condition (before nerve transection, for both groups)
• Test condition (after intervention for each group: sham surgery for sham group, nerve transection for PLNx group)
• Percentage of light‑induced voiding events (in %)
• Light‑induced voiding area (in cm²)
• Latency of voiding onset after light stimulation (in seconds)

File: Fig6_data.xlsx

Description: This file presents the source data related to the effects of photoactivation of estrogen receptor 1‑positive neurons in the pontine micturition center (PMC^ESR1+) on voiding‑related parameters before and after pudendal nerve transection (PDNx) in freely moving ESR1‑CRE mice. Data were collected from two groups: sham (control, no nerve transection) and pudendal nerve transection (PDNx). For each group, measurements were taken under two conditions: baseline (before any intervention) and post‑transection (after surgery for the PDNx group, or after sham surgery for the sham group). Sheet "Panel C" contains the percentage (%) of light‑induced voiding events in both groups. Sheet "Panel D" contains the light‑induced voiding area (in cm²) in both groups. Sheet "Panel E" contains the latency (in seconds) from light onset to voiding onset following light stimulation in both groups.

Variables

• Sham group (control, no nerve transection)
• Pudendal nerve transection group (PDNx)
• Baseline condition (before nerve transection, for both groups)
• Test condition (after intervention for each group: sham surgery for sham group, nerve transection for PDNx group)
• Percentage of light‑induced voiding events (in %)
• Light‑induced voiding area (in cm²)
• Latency of voiding onset after light stimulation (in seconds)

File: Fig7_data.xlsx

Description: This file presents the source data related to differences in anatomical projections to the lumbosacral spinal cord between corticotropin‑releasing hormone‑positive neurons in the pontine micturition center (PMC^CRH+) in CRH-CRE mice and estrogen receptor 1‑positive neurons in the pontine micturition center (PMC^ESR1+) in ESR1‑CRE mice using retrograde tracing. Sheet "Panel D" contains the number of PMC^CRH+ cells that project specifically to the sacral parasympathetic nucleus (SPN), specifically to the dorsal gray commissure (DGC), and to both SPN and DGC in the spinal cord. Sheet "Panel H" contains the number of PMC^ESR1+ cells that project specifically to the sacral parasympathetic nucleus (SPN), specifically to the dorsal gray commissure (DGC), and to both SPN and DGC in the spinal cord.

Variables

• Corticotropin‑releasing hormone‑positive neurons in the pontine micturition center (PMC^CRH+ neurons)
• Estrogen receptor 1‑positive neurons in the pontine micturition center (PMC^ESR1+ neurons)
• Projection to sacral parasympathetic nucleus in the lumbosacral spinal cord (SPN-projecting neurons)
• Projection to dorsal gray commissure (DGC) in the lumbosacral spinal cord (DGC-projecting neurons)
• Projection to both SPN and DGC (SPN/DGC‑projecting neurons)

File: Fig8_data.xlsx

Description: This file presents the source data related to the temporal coordination of bladder and external urethral sphincter (EUS) activity by estrogen receptor 1‑positive neurons in the pontine micturition center (PMCESR1+) in ESR1‑CRE mice. Sheet "Panel A" contains the temporal relationships (in seconds) among the onset times of calcium (Ca²⁺) signals (ΔF/F, % change from baseline), bladder pressure (BP) upstroke (onset of pressure rise), and EUS electromyography (EUS‑EMG) bursting onset in photometry recordings. Sheet "Panel B" contains the temporal relationships (in seconds) among the onset times of light stimulation, bladder pressure upstroke, and EUS‑EMG bursting onset in the photoactivation group (PMC^ESR1‑ChR2). Sheet "Panel C" contains the temporal relationships (in seconds) among the onset of light stimulation, the offset (termination) of EUS‑EMG bursting, and the offset (return to baseline) of bladder pressure upstroke in the photoinhibition group (PMC^{ESR1‑GtACR1}).

Variables

• Experimental condition (photometry recording, photoactivation, photoinhibition)
• Ca²⁺ signal onset time (in seconds)
• Light stimulation onset time (in seconds)
• Bladder pressure (BP) upstroke onset time (in seconds)
• Bladder pressure (BP) upstroke offset time (in seconds)
• EUS-EMG bursting onset time (in seconds)
• EUS-EMG bursting offset time (in seconds)

File: Fig8_S1_data.xlsx

Description: This file presents the source data related to the activity of dual‑projecting pontine micturition center (PMC) neurons during voiding, their correlations with bladder contraction and external urethral sphincter electromyography (EUS‑EMG) bursting, and the effects of their optogenetic activation on urination in ESR1‑CRE mice. Sheet "Panel C" contains the percentage (%) of voiding‑related Ca²⁺ transients (ΔF/F events occurring during voiding) in the GCaMP6f group (genetically encoded calcium indicator GCaMP6f). Sheet "Panel D" contains the amplitude of voiding‑related Ca²⁺ signals (ΔF/F, % change from baseline) in the GCaMP6f and shuffled groups (random permutation control). Sheet "Panel G" contains the data for the correlation coefficients between calcium (Ca²⁺) signals (expressed as ΔF/F, % change from baseline) and bladder contraction amplitude (in cm H₂O), along with the corresponding shuffled data. Sheet "Panel H" contains the data for the correlation coefficients between calcium (Ca²⁺) signals (expressed as ΔF/F, % change from baseline) and external urethral sphincter electromyography (EUS‑EMG) bursting events, along with the corresponding shuffled data. Sheet "Panel K" contains the latency (in seconds) from light onset to voiding onset. Sheet "Panel N" contains the percentage (%) of photoactivation‑associated bladder contraction and EUS‑EMG bursting events.

Variables

• Genetically encoded calcium indicator GCaMP6f group (GCaMP6f)
• Random permutation control group (shuffled)
• Percentage of voiding-related Ca²⁺ transients (in %)
• Voiding-related Ca²⁺ signal amplitude (ΔF/F, % change from baseline)
• Correlation coefficient with bladder contraction events (Ca²⁺ signals and shuffled data) 
• Correlation coefficient with EUS-EMG bursting events (Ca²⁺ signals and shuffled data) 
• Latency to voiding onset after light onset (in seconds)
• Percentage of photoactivation-associated bladder contractions (in %)
• Percentage of photoactivation-associated EUS-EMG bursting events (in %)

File: Fig8_S2_data.xlsx

Description: This file presents the source data related to the temporal coordination of bladder and external urethral sphincter (EUS) activity by dual‑projecting pontine micturition center (PMC) neurons (neurons that project to both the sacral parasympathetic nucleus and the dorsal gray commissure). Sheet "Panel A" contains the temporal relationships (in seconds) among the onset times of calcium (Ca²⁺) signals (ΔF/F, % change from baseline), bladder pressure (BP) upstroke (onset of pressure rise), and EUS electromyography (EUS‑EMG) bursting onset in photometry recordings. Sheet "Panel B" contains the temporal relationships (in seconds) among the onset times of light stimulation, bladder pressure upstroke, and EUS‑EMG bursting onset in the photoactivation group (PMC‑ChR2, light‑activated).

Variables

• Experimental condition (photometry recording, photoactivation)
• Ca²⁺ signal onset time (in seconds)
• Light stimulation onset time (in seconds)
• Bladder pressure (BP) upstroke onset time (in seconds)
• EUS-EMG bursting onset time (in seconds)

Code/software

The code to run this analysis is available on GitHub: https://github.com/xieyangshuying/Brainstem-neurons-coordinate-the-bladder-and-urethral-sphincter-for-urination.

Access information

Other publicly accessible locations of the data:

• N/A

Data was derived from the following sources:

• N/A