Data from: The SUbventral-Gland Regulator (SUGR) of nematode virulence
Data files
Jul 27, 2024 version files 2.08 MB
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2d_network_SUGR.gephi
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README.md
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SUGR_network_0.975.gexf
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Feb 10, 2025 version files 294.75 MB
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10855a(top_left)_13352a(top_right)_ns.tif
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10855a(top_left)_13352a(top_right)_s.tif
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13352b(top_left)_13352(top_right)_2729b(bottom_right)_ns.tif
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13352b(top_left)_13352(top_right)_2729b(bottom_right)_s.tif
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13352b(top_left)_13352(top_right)_2729b(bottom_right)_s(2).tif
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14352(top_right)_17860a(top_left)_satfb_b(bottom_right)_ns.tif
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14352(top_right)_17860a(top_left)_satfb_b(bottom_right)_s.tif
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14352(top_right)_17860a(top_left)_satfb_b(bottom_right)_s(2).tif
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14352b(top_left)_14352(top_right)_ns_(1).tif
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14352b(top_left)_14352(top_right)_s_(2).tif
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16818a(top_left)_13352b(top_right)_14352a(bottom_left)_10855b(bottom_right)_ns_.tif
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16818a(top_left)_13352b(top_right)_14352a(bottom_left)_10855b(bottom_right)_s.tif
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17860a(top_right)_17860b(top_left)_19185(bottom_right)_ns.tif
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17860a(top_right)_17860b(top_left)_19185(bottom_right)_s.tif
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17860a(top_right)_17860b(top_left)_19185(bottom_right)_s(2).tif
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18114b(top_left)_17860(bottom_left)_2729a(bottom_right)_s_(2).tif
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18114b(top_left)_17860(bottom_left)_2729a(bottom_right)_s.tif
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2d_network_SUGR.gephi
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Read_me.pdf
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README.md
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satfa_a(bottom_right)_16818(bottome_left)_s.tif
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satfa_a(bottom_right)_satfa_b_(bottom_left)_s.tif
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satfa_a(top_right)_satfa_b(bottom_right)_16818(bottom_left)_ns.tif
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satfa(top_left)_18114(top_right)_19185a(bottom_left)_16818b(bottom_right)_ns.tif
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satfa(top_left)_18114(top_right)_19185a(bottom_left)_16818b(bottom_right)_s.tif
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satfb(top_left)_16818b(top_right)_satfb_b(bottom_left)_satfb_a(bottom_right)_ns.tif
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satfb(top_left)_16818b(top_right)_satfb_b(bottom_left)_satfb_a(bottom_right)_s.tif
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SUGR_network_0.975.gexf
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Abstract
All pathogens must tailor their gene expression to their environment. Therefore, targeting host:parasite biology that regulates these changes in gene expression could open up routes to pathogen control. Here, we show that in the plant-parasitic nematode Heterodera schachtii, host signals (termed effectostimulins) within plant roots activate the master regulator sugr1. SUGR1, then, directly binds effector promoters, and orchestrates their production. Effector production, in turn, facilitates host entry, releasing more effectostimulins. These data show that gene expression during the very earliest stages of parasitism is defined by a feed forward loop for host entry. Importantly, we demonstrate that blocking SUGR1 blocks parasitism, underlining the SUGR1 signalling cascade as a valuable target for crop protection. Given that nematodes also parasitise humans and other animals, the potential impact is broad: disrupting effector production could, in principle, be applied to any pathogen that secrets effectors.
Transcriptional network files
Transcriptional network files for the plant-parasitic cyst nematode Heterodera schachtii showing correlations in gene expression across the life cycle (Siddique et al., 2022) between effector genes (as predicted in Molloy et al., 2024) and those endogenous H. schachtii transcription factors transcriptionally upregulated upon exposure to root extract (Pellegrin and Damm et al., 2024).
Description of the data and file structure
SUGR_network_0.975.gexf
Transcriptional network file showing correlations, above a threshold correlation coefficient of 0.975, between H. schachtii effector genes and root-extract upregulated H. schachtii transcription factors across the nematode life cycle.
0975_SUGR_TF_network_supercluster. gephi
Transcriptional network file allowing nodes to be coloured by various gene attributes such as supercluster (as defined by Siddique et al. (2022)), TF vs effector, root-extract upregulated effectors etc. The node “level” can also be split to show TFs and effectors in different planes, with the height of each TF correlated with number of connected effectors.
.gephi and .gexf network files can be opened in Gephi v0.10, which can be downloaded for free from: https://gephi.org.
Sharing/Access information
** **Instructions for generating transcriptional networks are available here: https://github.com/BethMolloy/Effectorome_H_schachtii and outlined in Molloy et al. (2024).
Yeast 1 Hybrid (Y1H) original images
Original pictures of yeast one hybrid screen shown in Figures S4-5. Binding of SUGR-1, SaTFa (SUGR activated Transcription Factor a), and SaTFb (SUGR activated Transcription Factor b) to promoters and promoter halves (proximal (b) and distal (a) halves) of SUGR-1 activated genes. Original pictures also show unpublished results for transcription factor x (not included in Figures S4-5 and the manuscript).
The associated "Read me.pdf" contains a description of the layout of each image such that with the name of the file and this layout, the contents of the image can be correctly deduced.
References
** **S. Siddique, et al., The genome and lifestage-specific transcriptomes of a plant-parasitic nematode and its host reveal susceptibility genes involved in trans-kingdom synthesis of vitamin B5. Nat. Commun. 13, 6190 (2022).
B. Molloy et al., The Origin, Deployment, and Evolution of a Plant-Parasitic Nematode Effectorome. BioRxiv. (2024).
C. Pellegrin and A. Damm et al., The SUbventral-Gland master regulator (SUGR) of nematode virulence. BioRxiv. (2024).
Version changes
09-Feb-2025. Added a section on Yeast 1 Hybrid (Y1H) original images
Instructions for the generation of transcriptional networks are available here: https://github.com/BethMolloy/Effectorome_H_schachtii
Instructions for the generation of Y1H original pictures are available in the associated Read me.pdf