Whole genome sequence data of Mycobacterium tuberculosis and Mycolicibacterium smegmatis mutants of the riboflavin biosynthetic pathway- Part 2
Data files
Jun 26, 2025 version files 57.88 GB
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1_S17_L004_R1_001.fastq.gz
1.40 GB
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1_S17_L004_R2_001.fastq.gz
1.45 GB
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2_S18_L004_R1_001.fastq.gz
1.70 GB
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2_S18_L004_R2_001.fastq.gz
1.77 GB
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3_S19_L004_R1_001.fastq.gz
1.28 GB
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3_S19_L004_R2_001.fastq.gz
1.34 GB
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4_S20_L004_R1_001.fastq.gz
1.30 GB
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4_S20_L004_R2_001.fastq.gz
1.36 GB
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5_S21_L004_R1_001.fastq.gz
1.40 GB
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5_S21_L004_R2_001.fastq.gz
1.48 GB
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6_S22_L004_R1_001.fastq.gz
1.26 GB
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6_S22_L004_R2_001.fastq.gz
1.31 GB
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7_S23_L004_R1_001.fastq.gz
1.20 GB
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7_S23_L004_R2_001.fastq.gz
1.27 GB
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Dobos_mtbribccomp10_S28_L002_R1_001.fastq.gz
2.93 GB
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Dobos_mtbribccomp10_S28_L002_R2_001.fastq.gz
2.95 GB
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Dobos_mtbribcko_11_S29_L002_R1_001.fastq.gz
1.79 GB
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Dobos_mtbribcko_11_S29_L002_R2_001.fastq.gz
1.78 GB
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Dobos_mtbribgcomp_14_S32_L002_R1_001.fastq.gz
2.50 GB
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Dobos_mtbribgcomp_14_S32_L002_R2_001.fastq.gz
2.48 GB
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Dobos_mtbribgko_15_S33_L002_R1_001.fastq.gz
2.32 GB
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Dobos_mtbribgko_15_S33_L002_R2_001.fastq.gz
2.35 GB
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Dobos_mtbribhcomp_12_S30_L002_R1_001.fastq.gz
2.38 GB
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Dobos_mtbribhcomp_12_S30_L002_R2_001.fastq.gz
2.38 GB
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Dobos_mtbribhko_13_S31_L002_R1_001.fastq.gz
2.10 GB
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Dobos_mtbribhko_13_S31_L002_R2_001.fastq.gz
2.12 GB
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Dobos_mtbrv3032_19_S37_L002_R1_001.fastq.gz
2.36 GB
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Dobos_mtbrv3032_19_S37_L002_R2_001.fastq.gz
2.40 GB
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Dobos_mtbwt_18_S36_L002_R1_001.fastq.gz
2.74 GB
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Dobos_mtbwt_18_S36_L002_R2_001.fastq.gz
2.77 GB
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README.md
4.56 KB
Abstract
This dataset contains raw whole-genome sequencing data (FASTQ files) for wild-type and riboflavin biosynthetic gene deletion mutants of Mycobacterium tuberculosis and Mycolicibacterium smegmatis. These strains were generated to investigate the source of metabolic antigens recognized by mucosal-associated invariant T (MAIT) cells, which detect intermediates from the riboflavin biosynthetic pathway. To confirm successful gene deletions and rule out off-target effects, whole-genome sequencing was performed for each mutant and its corresponding wild-type strain. This dataset supports the verification of targeted genetic modifications and enables comparative genomic analyses related to MR1 antigen production in mycobacteria.
Dataset DOI: 10.5061/dryad.vq83bk454
Description of the data and file structure
Whole Genome Sequence of wild type strain and riboflavin biosynthetic pathway mutants of Mycobacterium tuberculosis (Mtb) and Mycolicibacterium smegmatis (Msm). Also, this dataset has part 1 which is available at https://doi.org/10.5061/dryad.4tmpg4fnv
Naming convention for files:
For file names starting in digits
- Strain number (1: Wild Type, 2: *ΔfbiC, 3: ΔribH2, 4: ΔribH2 ΔribH1, 5: ΔribH2::ribH2, *6: ΔfbiC::fbiC, 7: ΔribH2 ΔribH:: ribH2 ribH1) 2. Sample Number 3. Read (Forward/Reverse) 6. Replicate
e.g. For file name "7_S23_L004_R2_001"Strain number: 7 (ΔribH2 ΔribH:: ribH2 ribH1); Sample Number: S23; Read: R2 (Reverse Read); Replicate: 001
OR
For file names starting in "Dobos"
- Lab name 2. Strain Name 3. Sample Number 4. Batch Number 5. Read (Forward/Reverse) 6. Replicate
e.g. For file name "Dobos_msmegriba2comp_7_S25_L002_R2_001": Lab name: Dobos; Strain Name: msmegriba2comp_7; Sample Number: S25; Batch Number: L002; Read: R2 (Reverse Read); Replicate: 001
Files and variables
File: 7_S23_L004_R2_001.fastq.gz
Description: Reverse Read for Msm ΔribH2 ΔribH1::ribH2 ribH1
File: 7_S23_L004_R1_001.fastq.gz
Description: Forward Read for Msm ΔribH2 ΔribH1::ribH2 ribH1
File: 6_S22_L004_R1_001.fastq.gz
Description: Forward Read for Msm ΔfbiC::fbiC
File: 6_S22_L004_R2_001.fastq.gz
Description: Reverse Read for Msm ΔfbiC::fbiC
File: 4_S20_L004_R1_001.fastq.gz
Description: Forward Read for Msm ΔribH2 ΔribH1
File: 4_S20_L004_R2_001.fastq.gz
Description: Reverse Read for Msm ΔribH2 ΔribH1
File: 5_S21_L004_R1_001.fastq.gz
Description: Forward Read for Msm ΔribH2::ribH2
File: 3_S19_L004_R2_001.fastq.gz
Description: Forward Read for Msm ΔribH2
File: 3_S19_L004_R1_001.fastq.gz
Description: Reverse Read for Msm ΔribH2
File: 5_S21_L004_R2_001.fastq.gz
Description: Reverse Read for Msm ΔribH2::ribH2
File: 1_S17_L004_R1_001.fastq.gz
Description: Forward Read for Msm Wild Type
File: 1_S17_L004_R2_001.fastq.gz
Description: Reverse Read for Msm Wild Type
File: 2_S18_L004_R1_001.fastq.gz
Description: Forward Read for Msm ΔfbiC
File: 2_S18_L004_R2_001.fastq.gz
Description: Reverse Read for Msm ΔfbiC
File: Dobos_mtbwt_18_S36_L002_R2_001.fastq.gz
Description: Reverse Read for Mtb Wild Type H37Rv
File: Dobos_mtbwt_18_S36_L002_R1_001.fastq.gz
Description: Forward Read for Mtb Wild Type H37Rv
File: Dobos_mtbrv3032_19_S37_L002_R2_001.fastq.gz
Description: Reverse Read for Mtb Wild Type Rv3032
File: Dobos_mtbrv3032_19_S37_L002_R1_001.fastq.gz
Description: Forward Read for Mtb Wild Type Rv3032
File: Dobos_mtbribhko_13_S31_L002_R2_001.fastq.gz
Description: Reverse Read for Mtb ΔribH
File: Dobos_mtbribhko_13_S31_L002_R1_001.fastq.gz
Description: Forward Read for Mtb ΔribH
File: Dobos_mtbribhcomp_12_S30_L002_R2_001.fastq.gz
Description: Reverse Read for Mtb ΔribH::ribH
File: Dobos_mtbribhcomp_12_S30_L002_R1_001.fastq.gz
Description: Forward Read for Mtb ΔribH::ribH
File: Dobos_mtbribgko_15_S33_L002_R2_001.fastq.gz
Description: Reverse Read for Mtb ΔribG
File: Dobos_mtbribgko_15_S33_L002_R1_001.fastq.gz
Description: Forward Read for Mtb ΔribG
File: Dobos_mtbribgcomp_14_S32_L002_R2_001.fastq.gz
Description: Reverse Read for Mtb ΔribG::ribG
File: Dobos_mtbribgcomp_14_S32_L002_R1_001.fastq.gz
Description: Forward Read for Mtb ΔribG::ribG
File: Dobos_mtbribcko_11_S29_L002_R2_001.fastq.gz
Description: Reverse Read for Mtb ΔribC
File: Dobos_mtbribcko_11_S29_L002_R1_001.fastq.gz
Description: Forward Read for Mtb ΔribC
File: Dobos_mtbribccomp10_S28_L002_R2_001.fastq.gz
Description: Reverse Read for Mtb ΔribC::ribC
File: Dobos_mtbribccomp10_S28_L002_R1_001.fastq.gz
Description: Forward Read for Mtb ΔribC::ribC