Qualification of ELISA and PRNT methodologies to measure RSV humoral immunity using human clinical samples
Data files
Sep 24, 2026 version files 27.32 KB
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File_descriptions.csv
1.12 KB
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README.md
4.15 KB
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RSV_qual_sample_metadeta.xlsx
12.25 KB
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RSV_strain_A_post-F_IgA_EPT.csv
1.31 KB
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RSV_strain_A_pre-F_IgA_EPT.csv
1.39 KB
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RSV_strain_A_pre-F_IgG_EPT.csv
1.35 KB
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RSV_strain_B_post-F_IgA_EPT.csv
1.23 KB
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RSV_strain_B_pre-F_IgA_EPT.csv
1.38 KB
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RSV_strain_B_pre-F_IgG_EPT.csv
1.35 KB
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RSV_strain_B_PRNT50_mAbs.csv
77 B
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RSV_strain_B_PRNT50_pre-F_IgG_EPT.csv
1.15 KB
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RSV_strain_B_PRNT50.csv
564 B
Abstract
Respiratory Syncytial Virus (RSV) represents a substantial public health burden worldwide, causing lower respiratory infections primarily in children under 5 and adults over 65. In recent years vaccines and monoclonal antibodies have demonstrated promising prophylaxis against RSV in vulnerable populations, benefitting individuals, families, and healthcare systems. However, this protection is incomplete, highlighting the importance of continued research and development towards novel and improved therapeutics. Clinically qualified endpoint assays are a crucial tool in these efforts, giving researchers insight into the magnitude, composition, and neutralization efficacies of the humoral immune response to RSV across clinical evaluations. We clinically qualified two correlated RSV assays, an Enzyme Linked Immunosorbent Assay (ELISA) and a Plaque Reduction Neutralization Test (PRNT). We demonstrate each assay is precise, linear, and translational. Publicly available controls increase reproducibility and transferability to other laboratories; to that end, here we provide additional information specific to each sample we evaluated, as well as the results of each ELISA and PRNT assay run for each sample.
Dataset DOI: 10.5061/dryad.w6m905r40
Description of the data and file structure
EPT values and PRNT50 values generated in the clinical qualification of ELISA and PRNT methodologies, respectively, are provided here. File_descriptions.csv contains descriptions of each file, while raw values for each sample within each set of test parameters are included within each file. RSV_qual_sample_metadeta contains additional information pertaining to each sample.
Files and variables
File: RSV_strain_B_PRNT50_mAbs.csv
Description: PRNT50 values from PRNTs evaluating mAb neutralization to RSV strain B, expressed as ng/mL.
Variables
- Sample
- PRNT50, ng/mL
File: RSV_strain_A_post-F_IgA_EPT.csv
Description: EPT values from ELISAs evaluating IgA response to RSV strain A post-F antigen.
Variables
- Sample
- Day/replicate/operator: D1, D2, and D3 indicate on which day the assay was performed. D2_1, D2_2, and D2_3 indicate the replicate in question on day 2. D3_1, D3_2, and D3_3 indicate which operator performed the assay on day 3.
File: RSV_qual_sample_metadeta.xlsx
Description: Additional information pertaining to each sample assessed in this qualification.
Variables
- Sample
- Source
- Catalogue number
- Lot number/sample designation
File: RSV_strain_B_PRNT50_pre-F_IgG_EPT.csv
Description: PRNT50 values and EPT values evaluating IgG response to RSV strain B and RSV strain B pre-F antigen, respectively, in pediatric serum samples.
Variables
- Sample
- EPT
- PRNT50
File: RSV_strain_A_pre-F_IgA_EPT.csv
Variables
- Sample
- Day/replicate/operator: D1, D2, and D3 indicate on which day the assay was performed. D2_1, D2_2, and D2_3 indicate the replicate in question on day 2. D3_1, D3_2, and D3_3 indicate which operator performed the assay on day 3.
File: RSV_strain_A_pre-F_IgG_EPT.csv
Variables
- Sample
- Day/replicate/operator: D1, D2, and D3 indicate on which day the assay was performed. D2_1, D2_2, and D2_3 indicate the replicate in question on day 2. D3_1, D3_2, and D3_3 indicate which operator performed the assay on day 3.
File: RSV_strain_B_post-F_IgA_EPT.csv
Variables
- Sample
- Day/replicate/operator: D1, D2, and D3 indicate on which day the assay was performed. D2_1, D2_2, and D2_3 indicate the replicate in question on day 2. D3_1, D3_2, and D3_3 indicate which operator performed the assay on day 3.
File: RSV_strain_B_pre-F_IgA_EPT.csv
Variables
- Sample
- Day/replicate/operator: D1, D2, and D3 indicate on which day the assay was performed. D2_1, D2_2, and D2_3 indicate the replicate in question on day 2. D3_1, D3_2, and D3_3 indicate which operator performed the assay on day 3.
File: RSV_strain_B_pre-F_IgG_EPT.csv
Variables
- Sample
- Day/replicate/operator: D1, D2, and D3 indicate on which day the assay was performed. D2_1, D2_2, and D2_3 indicate the replicate in question on day 2. D3_1, D3_2, and D3_3 indicate which operator performed the assay on day 3.
File: RSV_strain_B_PRNT50.csv
Variables
- Sample
- Day/replicate/operator: D1, D2, and D3 indicate on which day the assay was performed. D2_1, D2_2, and D2_3 indicate the replicate in question on day 2. D3_1, D3_2, and D3_3 indicate which operator performed the assay on day 3.
File: File_descriptions.csv
Description: Table of contents describing the contents of each file for ease of access.
Variables
- File name
- Description
Code/software
This data can be viewed by any program capable of viewing .csv files.
Human subjects data
Samples in the Coler Lab Biorepository were drawn from Seattle Children's workforce and adult community members with informed consent and Institutional Review Board approval through Seattle Children's Research Institute. Data was de-identified by assigning study-specific alphanumeric labels to the samples without any personally identifiable information.