This dataset describes the diet of a highly diverse tropical anuran assemblage. We report the diet composition of 35 species collected at Yasuní National Park, based on the examination of the gastrointestinal contents of 396 frog specimens available in the Museo de Zoología (QCAZ) of Pontificia Universidad Católica del Ecuador. Anuran species belong to six families: Aromobatidae, Bufonidae, Dendrobatidae, Hylidae, Leptodactylidae, and Strabomantidae. We were able to identify 4,085 prey items that were classified into 71 categories. We present information on anuran-prey interactions, prey-items volume, and anuran specimens' associated data. This data set contributes to understanding the trophic ecology of anuran species and their role in tropical ecosystems.

We extracted the gastrointestinal contents of 396 adult frogs. Using a stereoscopic microscope, we identified prey items to the lowest taxonomic level possible and estimated their volume (mm³) based on the length and width of intact prey items. The number of specimens examined per species varied according to their availability at the museum collection.

No special programs or software are required to open the data flies.