Experimental glomerular sieving coefficients for neutral and anionic polysucrose during baseline filtration and hyperfiltration in Wistar rats
Data files
Jul 28, 2026 version files 220.92 KB
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full.xls
182.27 KB
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README.md
5.37 KB
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reduced.xls
33.28 KB
Abstract
This dataset contains experimental measurements of glomerular sieving coefficients for neutral and anionic polysucrose (Ficoll) in healthy anesthetized Wistar rats under baseline conditions and during glucagon-induced hyperfiltration. Glomerular filtration rate was determined using both FITC-inulin and 51Cr-EDTA clearance. Sieving coefficients were measured across a broad range of hydrodynamic radii using high-performance size-exclusion chromatography (HPSEC), providing detailed glomerular sieving curves spanning approximately five orders of magnitude in permeability.
The study employed a 2 × 2 factorial design with two polysucrose charge states (neutral and anionic) and two filtration states (baseline and hyperfiltration). Hyperfiltration was induced by glucagon infusion combined with volume loading. The dataset includes raw chromatographic data, processed sieving coefficients, glomerular filtration rate measurements, and derived molecular radius calibrations.
These data may be useful for studies of glomerular size and charge selectivity, macromolecular transport, pore-theory modeling, electrostatic effects in biological filtration barriers, dimensionality reduction, principal component analysis, and the development and validation of mechanistic models of glomerular filtration.
Dataset DOI: 10.5061/dryad.wh70rxx45
Description of the data and file structure
This dataset contains experimental measurements of glomerular permeability obtained in healthy male Wistar rats under baseline conditions and during glucagon-induced hyperfiltration.
Glomerular sieving coefficients were measured using fluorescently labeled neutral polysucrose and negatively charged (anionic) polysucrose spanning a broad range of hydrodynamic radii. Glomerular filtration rate (GFR) was determined using FITC-inulin and 51Cr-EDTA clearance. Animals were studied during baseline conditions and after induction of hyperfiltration by glucagon infusion combined with volume expansion.
The dataset consists of two Microsoft Excel files:
reduced.xls contains the reduced dataset used for nonlinear mixed-effects (NLME) model fitting. This file includes physiological variables, glomerular filtration rate measurements, and a subset of representative polysucrose sieving coefficients selected for computationally efficient estimation of distributed two-pore model parameters.
full.xls contains the complete set of measured glomerular sieving coefficients for all available polysucrose radii. This file was used for correlation analyses, principal component analyses, and exploratory analyses of the dimensional structure of glomerular transport. Column names beginning with “GFR” identify individual animals (1-6: neutral polysucrose, 8-15: charge-modified polysucrose). Columns ending with “b” represent baseline measurements, whereas columns without a suffix represent measurements obtained during hyperfiltration. The variable “radius” denotes the hydrodynamic radius of the polysucrose fraction in Ångström units.
The dataset accompanies the manuscript: “Glomerular Hyperfiltration, Charge Selectivity, and the Low-Dimensional Structure of Glomerular Transport”.
Files and variables
File: reduced.xls
Description
Reduced dataset used for nonlinear mixed-effects (NLME) regression of the electrostatic distributed two-pore model. The file contains physiological variables, glomerular filtration rate measurements, and representative polysucrose sieving coefficients selected for computationally efficient model fitting.
Variables
id
Unique observation identifier.
subject
Animal identifier.
group
Experimental group:
- neutral = neutral polysucrose
- charged = anionic (charge-modified) polysucrose
treatment
Experimental condition:
- normal = baseline filtration
- hyperfiltration = glucagon-induced hyperfiltration
bp
Mean arterial blood pressure (mmHg).
hr
Heart rate (beats per minute).
gfr
Glomerular filtration rate determined by 51Cr-EDTA clearance (mL/min).
inulin
Glomerular filtration rate determined by FITC-inulin clearance (mL/min).
f20
Glomerular sieving coefficient (θ) for polysucrose with hydrodynamic radius 2.0 nm.
f25
Glomerular sieving coefficient (θ) for polysucrose with hydrodynamic radius 2.5 nm.
f30
Glomerular sieving coefficient (θ) for polysucrose with hydrodynamic radius 3.0 nm.
f36
Glomerular sieving coefficient (θ) for polysucrose with hydrodynamic radius 3.6 nm.
f40
Glomerular sieving coefficient (θ) for polysucrose with hydrodynamic radius 4.0 nm.
f45
Glomerular sieving coefficient (θ) for polysucrose with hydrodynamic radius 4.5 nm.
f50
Glomerular sieving coefficient (θ) for polysucrose with hydrodynamic radius 5.0 nm.
f55
Glomerular sieving coefficient (θ) for polysucrose with hydrodynamic radius 5.5 nm.
f64
Glomerular sieving coefficient (θ) for polysucrose with hydrodynamic radius 6.4 nm.
f80
Glomerular sieving coefficient (θ) for polysucrose with hydrodynamic radius 8.0 nm.
Missing values
No missing values are present in the reduced dataset.
File: full.xls
Description
Complete dataset containing glomerular sieving coefficients for all measured polysucrose radii. This file was used for correlation analyses, principal component analyses, dimensionality reduction, and exploratory analyses of glomerular transport structure.
Variables
radius
Hydrodynamic radius of the polysucrose fraction (Å).
GFR1b–GFR15b
Glomerular sieving coefficients (θ) measured during baseline filtration conditions. The number identifies the animal. Animals 1–6 received neutral polysucrose and animals 8, 9, 12, 13, 14 and 15 received anionic polysucrose.
GFR1–GFR15
Glomerular sieving coefficients (θ) measured during glucagon-induced hyperfiltration. The number identifies the animal. Animals 1–6 received neutral polysucrose and animals 8, 9, 12, 13, 14 and 15 received anionic polysucrose.
Units
radius: Ångström (Å)
GFR columns: dimensionless glomerular sieving coefficient (θ)
Experimental groups
Animals 1–6:
Neutral polysucrose.
Animals 8, 9, 12, 13, 14 and 15:
Anionic (charge-modified) polysucrose.
Suffix “b”:
Baseline measurements.
No suffix:
Hyperfiltration measurements.
Missing values
Animals 7, 10 and 11 were excluded from the study because of experimental complications and are therefore not present in the dataset.
Code/software
Microsoft Excel