Data from: Increased CA3 burst activity in Doc2α and Syt7 knockout mice
Data files
Jul 09, 2026 version files 366.84 MB
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CA1_Current_Clamp_I___0_Figure_1.zip
136.79 MB
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CA1_Voltage_Clamp___-70mV_Figure_1.zip
156.89 MB
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CA3_Current_Clamp____-70mV_Figure_2.zip
73.16 MB
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README.md
2.56 KB
Abstract
Double C2 domain-containing protein α (Doc2α) and synaptotagmin 7 (Syt7) are high-affinity calcium sensors implicated in asynchronous synaptic vesicle (SV) release and in the exocytosis of dense-core vesicles (DCVs). Additionally, Doc2α is a sensor for miniature neurotransmission, whereas Syt7 is involved in synaptic facilitation and SV replenishment. Doc2α and Syt7 are expressed in the hippocampus, but their potential roles in spontaneous excitatory network activity remain unanswered. Using whole-cell recordings in disinhibited acute hippocampal slices obtained from juvenile Doc2α- and Syt7- knockout (KO) mice (P15-21), we report increased CA3 burst generation without changes in spontaneous excitatory postsynaptic current (sEPSC) frequency or amplitude. Moreover, the intrinsic properties of CA3 pyramidal neurons, such as the resting membrane potential, firing rate and input resistance, are unchanged. The data set provided in this submission includes all raw data files from whole-cell voltage clamp and current clamp recordings.
Dataset DOI: 10.5061/dryad.wpzgmsc50
Description of the data and file structure
The data were collected to test the role of presynaptic proteins Doc2α and Syt7 in regulating excitatory network activity. To test the hypothesis, we used knockout mice. The data include whole-cell voltage-clamp and current-clamp recordings from acute hippocampal slices.
There are three ZIP files
a) CA1_Current_Clamp_I_=_0_Figure_1
b) CA1_Voltage_Clamp_@_-70mV_Figure_1
c) CA3_Current_Clamp_@_~-70mV_Figure_2
Each ZIP file contains the four study groups
i) WT (Wild type)
ii) Doc2α_KO (Doc2α-/-)
iii) Syt7_KO (Syt7-/-) and
iv) Doc2α_Syt7_DKO (Doc2α-/-/Syt7-/-).
Within each group, the data were organized as folders with names denoting the date of the experiment and sex of the mice as follows.
Mice_Number_Date_of_Experiment_Sex_of_the_mice
Each subgroup has unprocessed axon binary files (ABF, file extension = .abf)
The ABF files were named by the software (Clampex v11.3, part of pClamp Suite 11, Molecular Devices) during acquisition as YEAR_Month_Date_XXXX, where XXXX denotes the file number on the day of recording.
Files and variables
File: CA1_Current_Clamp_I___0_Figure_1.zip
Description: Current clamp recordings performed in CA1 neurons at resting membrane potential (I = 0 mode) to study the burst phenotype
File: CA1_Voltage_Clamp___-70mV_Figure_1.zip
Description: Voltage clamp recordings performed in CA1 neurons at -70 mV to study the frequency and amplitude of spontaneous excitatory postsynaptic currents
File: CA3_Current_Clamp____-70mV_Figure_2.zip
Description: Current clamp recordings performed in CA3 neurons, approximately at -70 mV membrane potential, to assess the intrinsic properties of the neurons
Code/software
The raw data files are AXON binary files (.abf). You need to download and install Clampfit software (part of the pClamp suite). Check the Molecular Devices support website. https://support.moleculardevices.com/s/article/Axon-pCLAMP-11-Electrophysiology-Data-Acquisition-Analysis-Software-Download-Page
Access information
Other publicly accessible locations of the data:
- N/A
Data was derived from the following sources:
- N/A