Data from: Development of a reliable PCR-based protocol for molecular sexing of king vultures Sarcoramphus papa
Data files
Jul 15, 2026 version files 17.62 KB
Abstract
This dataset accompanies the manuscript "Development of a Reliable PCR-Based Protocol for Molecular Sexing of King Vultures Sarcoramphus papa". It contains sample metadata, NanoDrop DNA concentration and purity measurements for DNA extracted from whole blood, blood preserved on Whatman FTA cards, and plucked feathers, and molecular sex assignments obtained using three CHD1 primer sets (2550F/2718R, NP/1272H, and NP/MP/P2). The dataset supports the evaluation of PCR-based molecular sexing methods for S. papa.
Dataset DOI: 10.5061/dryad.wwpzgmt1g
Description of the data and file structure
This dataset was generated to evaluate PCR-based molecular sexing in king vultures (Sarcoramphus papa) using three CHD1 primer sets (2550F/2718R, NP/1272H, and NP/MP/P2). The dataset includes sample metadata, NanoDrop DNA concentration and purity measurements for DNA extracted from whole blood, blood preserved on Whatman FTA cards, and plucked feathers, and the resulting molecular sex assignments for eight individuals. These data support the analyses and conclusions presented in the associated manuscript.
Files and variables
File: Pozo_et_al_2026_King_Vulture_Molecular_Sexing_Supporting_Data.xlsx
Description:
This Excel workbook contains the supporting data associated with the manuscript Development of a Reliable PCR-Based Protocol for Molecular Sexing of King Vultures (Sarcoramphus papa). The workbook is organized into four data sheets:
- README: metadata for the other data sheets.
- Sample metadata: Individual identification numbers, original capture codes, and sample types.
- NanoDrop measurements: DNA concentration (ng/µL) and purity measurements (A260/280 and A260/230) for DNA extracted from whole blood, blood preserved on Whatman FTA cards, and plucked feathers.
- Molecular sex assignment: Molecular sex determined for each individual using the three CHD1 primer sets evaluated in this study.
Variables
Sample metadata
- Individual: Individual identification number corresponding to lane numbers in Figures 1, 2, S1 and S2.
- Capture code: Original field sample identifier.
- Sample type: Biological sample analyzed (whole blood, Whatman FTA card, or plucked feather).
NanoDrop measurements
- Individual: Individual identification number.
- Capture code: Original field sample identifier.
- Sample type: DNA source.
- DNA concentration (ng/µL): DNA concentration measured using a NanoDrop spectrophotometer.
- A260/280: DNA purity ratio.
- A260/230: DNA purity ratio.
Molecular sex assignment
- Individual: Individual identification number.
- Capture code: Original field sample identifier.
- Primer Set 1 (2550F/2718R): Molecular sex assignment.
- Primer Set 2 (NP/1272H): Molecular sex assignment.
- Primer Set 3 (NP, MP, P2): Molecular sex assignment.
Missing values
No missing values are present in this dataset.
Code/software
No custom code or software is required to use this dataset. The data are provided in Microsoft Excel (.xlsx) format and can be viewed using Microsoft Excel or compatible spreadsheet software such as LibreOffice Calc or Google Sheets.
Access information
Other publicly accessible locations of the data:
- None
Data was derived from the following sources:
- The data are original and were generated as part of the study described in the associated manuscript. They were not derived from previously published datasets.