Dataset DOI: 10.5061/dryad.x3ffbg80q

Description of the data and file structure

File: Supplemental_Raw_Data-H._Ahmad.zip

The dataset contains characterizations related data of composite and intermediates obtained from Fourier Transform IR (FTIR) spectra, X-ray photoelectron spectroscopy (XPS) survey spectra, X-ray diffraction (XRD) patterns, magnetic moments measurements against applied field using a Micro Sense vibrating sample magnetometer (VSM), temperature and pH-dependent change in average hydrodynamic diameters by a NICOMP 380 particle sizer, zeta potential variation with pH using a Malvern zeta analyzer, and mass loss with temperature increment by a Thermogravimetry Analyzer (TGA). Loading and release behavior – loading of dyes, encapsulation, and release of biomolecules and drugs were assessed from absorbance values using a UV-visible spectrophotometer.

Extended_Data _Figure_3.x1sx
Description: This file provides FTIR data used to follow step-by-step change in surface functionality of iron oxide nanoparticles during preparation of mFe3O4/mSiO2/P(NIPAM-CEA-MBA) composite.
Variables
• Wavenumber (cm⁻¹) – vibration frequency of bonds.
• Transmittance (%) – amount of light passing through the sample.
• Peak Height / Area (a.u.) – relative intensity of functional groups.

Extended_Data _Figure_4.x1sx
Description: This file shows XPS survey spectral data used for confirming elemental compositions during the stepwise surface modification of iron oxide nanoparticles.
Variables
• Binding Energy (eV) – energy of photoelectrons emitted from the sample surface.
• Intensity (a.u.) – relative XPS signal intensity (arbitrary units) of each element or chemical state.
• Element / Peak Assignment – identifies the element and its chemical state for each coating step (Fe, O, Si, C, etc.).

Extended_Data _Figure_5.x1sx
Description: This file provides data related to X-ray diffraction patterns of mFe3O4 particles, mFe3O4/mSiO2 core-particles and mFe3O4/mSiO2/P(NIPAM-CEA-MBA) composite.
Variables
• 2θ (degree) – diffraction angle in degrees (2θ).
• Intensity (a.u.) – relative X-ray intensity (arbitrary units).
• Crystallite Size (nm) – size of coherent diffracting domains calculated from peak broadening.
• Crystallinity (%) – degree of crystallinity of the sample.

Extended_Data _Figure_6.x1sx
Description: This file provides change in magnetization values with applied field measured by a Vibrating Sample Magnetometer (VSM) for mFe3O4 particles, mFe3O4/mSiO2 core-particles and mFe3O4/mSiO2/P(NIPAM-CEA-MBA) composite.
Variables
• H (Oe) – applied magnetic field.
• M (emu/g) – magnetization per gram of sample.

Extended_Data _Figure_7a.x1sx
Description: This file depicts the relevant data describing the effect of temperature increase on the average hydrodynamic diameter of the mFe₃O₄/mSiO₂/P(NIPAM-CEA-MBA) composite at pH 8.0 and 10.0, indicating how particle size changes with temperature at different pH levels.
Variables:
• Temperature (°C) — The temperature at which the hydrodynamic diameter was measured.
• Average Hydrodynamic Diameter (nm) — The effective size of the composite particles in solution.
• pH (8.0 and 10.0) — The pH of the composite suspension adjusted before the measurement.

Extended_Data _Figure_7b.x1sx
Description: This file shows data related to the change in zeta potential with pH for the mFe₃O₄/mSiO₂/P(NIPAM-CEA-MBA) composite.
Variables
• pH — The acidity or basicity of the solution during measurement.
• Zeta Potential (mV) — The surface charge of the composite particles.

Extended_Data _Figure_8A.x1sx
Description: This file gives change in loading amount on the mFe3O4/mSiO2/P(NIPAM-CEA-MBA) composite for cationic crystal violet (CV) dye against time (min). Variables
• Time (min)
Contact time between the CV dye solution and the composite material during the loading process.
• Dye loading amount (mg g⁻¹)
Amount of CV loaded per gram of composite, calculated from UV–visible absorbance measurements.
• Temperature (°C)
Experimental loading temperature (25° and 40°C).
• pH
pH of the dye solution during loading (pH 5.0 and pH 10.0).
• Initial dye concentration (0.02 mg mL⁻¹)
• Solution volume (mL)
Total volume of dye solution used in the loading experiment (100 mL).

Extended_Data _Figure_8B.x1sx
Description: This file gives data of loading amount of anionic congo red (CR) on mFe3O4/mSiO2/P(NIPAM-CEA-MBA) composite against time (min) under different pH and temperature conditions.

Variables
• Time (min)
Contact time between the CR dye solution and the composite during the loading process.
• Dye loading amount (mg g⁻¹)
Amount of CR dye loaded per gram of composite, calculated from UV–visible absorbance measurements.
• Temperature (°C)
Experimental loading temperature (25 °C and 40 °C).
• pH
pH of the dye solution during loading (pH 5.0 and pH 10.0).
• Initial dye concentration (0.02 mg mL⁻¹).
• Solution volume (mL)
Total volume of dye solution used in the loading experiment (100 mL).
File :

Extended_Data _Figure_9a.x1sx
Description: The dataset contains data for the loading, encapsulation, and pH-temperature controlled release experiments of lysozyme (LZ) from mFe3O4/mSiO2/P(NIPAM-CEA-MBA) composite. These data are calculated from UV–visible spectrophotometric measurements.
Variables
• Time (min) – duration of loading or release.
• Absorbance (a.u.) – UV–visible absorbance at 280 nm.
• Lysozyme concentration (mg L⁻¹) – calculated from calibration curve.
• Initial LZ amount (mg g⁻¹) – added per gram of composite.
• Encapsulated lysozyme (mg g⁻¹) – retained in composite.
• Released lysozyme (mg g⁻¹).
• Encapsulation efficiency (%) – % of LZ encapsulated.
• Cumulative release (%) – % of LZ released.

Extended_Data _Figure_9b.x1sx
Description: The dataset gives the loading, encapsulation, and pH-temperature-controlled release experiments of vancomycin (VC) from mFe3O4/mSiO2/P(NIPAM-CEA-MBA) composite. These data are calculated from UV–visible spectrophotometric measurements.
Variables
• Time (min) – duration of loading or release.
• Absorbance (a.u.) – UV–visible absorbance at 280 nm.
• VC concentration (mg L⁻¹) – calculated from calibration curve.
• Initial VC amount (mg g⁻¹) – added per gram of composite.
• Encapsulated VC (mg g⁻¹) – retained in composite.
• Released VC (mg g⁻¹) from composite.
• Encapsulation efficiency (%) – % of VC encapsulated.
• Cumulative release (%) – % of VC released.

Extended_Data _Figure_9c.x1sx
Description: The dataset provides data for the loading, encapsulation, and pH-temperature-controlled release experiments of clonazepam (CZ) from mFe3O4/mSiO2/P(NIPAM-CEA-MBA) composite. These data are calculated from UV–visible spectrophotometric measurements.
Variables
• Time (min) – duration of the loading or release experiment.
• Absorbance (a.u.) – UV–visible absorbance intensity of CZ measured at 261 nm.
• CZ concentration (mg L⁻¹) – calculated from the calibration curve.
• Initial CZ amount (mg g⁻¹) – added per gram of composite during loading.
• Encapsulated CZ (mg g⁻¹) – amount retained within the composite after encapsulation.
• Released clonazepam (mg g⁻¹) – amount released from the composite at a given time.
• Encapsulation efficiency (%) – percentage encapsulated relative to the initial amount.
• Cumulative release (%) – percentage released relative to the encapsulated amount.

Extended_Data _Figure_10a.x1sx
Description: This file depicts the absorbance values obtained from supernatants of composite-RBC suspension during concentration-dependent (0.4, 0.5, 0.6, and 0.7 mg mL-1) hemolytic activity measurements of mFe3O4/mSiO2/P(NIPAM-CEA-MBA) composite.
Variables
• Composite concentration (mg mL-1) – variable concentrations used (0.4, 0.5, 0.6, 0.7 mg mL-1).
• Absorbance of supernatant - absorbance value of supernatant from composite-RBC suspension at 540 nm
• Positive and negative control – positive and negative control experiments in respective 1% aqueous TritonX-100 and 0.9% aqueous NaCl (physiological saline) solution
• Hemolysis (%) – percentage of red blood cells lysed relative to the positive control, calculated from absorbance value using equation (2)

Extended_Data _Figure_10b.x1sx
Description: This file provides the time-dependent hemolysis percentages of the composite at 0.5 mg mL-1 concentration.
Variables
• Time (min) – incubation time during hemolysis testing (60, 90, 120 min).
• Hemolysis (%) – percentage of red blood cell lysis observed at each time interval.

Supplementary_Figure_S1a.x1sx
Description: This file provides the calibration curve related absorbance data at 581 nm for crystal violet (CV) measured at pH 5.00.
Variables
• Concentration (mg mL⁻¹) – concentration (mg mL⁻¹) of CV in aqueous solution at pH 5.00.
• Absorbance (581 nm) – UV–Vis absorbance intensity of CV measured at 581 nm
(dimensionless).
• Linear fit (y = 94.418x) – linear regression equation correlating absorbance with
concentration.
• R² – coefficient of determination indicating the goodness of fit of the calibration curve.

Supplementary _Figure_S1b.x1sx
Description: This data presents calibration curve related absorbance values at 581 nm for different concentrations of crystal violet (CV) measured at pH 10.00.
Variables
• Concentration (mg mL⁻¹) – Concentration (mg mL⁻¹) of CV in aqueous solution at pH 10.00.
• Absorbance (581 nm) – UV–Vis absorbance intensity of CV measured at 581 nm
(dimensionless).
• Linear fit (y = 58.874x) – Linear regression equation correlating absorbance with
concentration.
• R² – Coefficient of determination indicating the goodness of fit of the calibration curve.

Supplementary_Figure_S2a.x1sx
Description: This file gives calibration curve related experimental absorbance values at different concentrations of congo red (CR) recorded at pH 5.00 obtained at 340 nm wavelength.
Variables
• Concentration (mg mL⁻¹) – Concentration (mg mL⁻¹) of CR in aqueous solution at pH 5.00.
• Absorbance (340 nm) – UV–Vis absorbance intensity of CR measured at 340 nm
(dimensionless).
• Linear fit (y = 7.8863x) – Linear regression equation correlating absorbance with
concentration.
• R² – Coefficient of determination indicating the goodness of fit of the calibration curve.

Supplementary _Figure_S2b.x1sx
Description: This file gives calibration curve related experimental absorbance values at different concentrations of congo red (CR) recorded at pH 10.00 obtained at 490 nm wavelength.
Variables
• Concentration (mg mL⁻¹) – Concentration (mg mL⁻¹) of CR in aqueous solution at pH 10.00.
• Absorbance (490 nm) – UV–Vis absorbance intensity of CR measured at 490 nm
(dimensionless).
• Linear fit (y = 47.899x) – Linear regression equation correlating absorbance with
concentration.
• R² – Coefficient of determination indicating the goodness of fit of the calibration curve.

Supplementary_Figure_S3a.x1sx
Description: This file gives the UV–Vis calibration curve related absorbance data for variable concentrations of lysozyme (LZ) at pH 5.00 measured at 280 nm wavelength.
Variables
• Concentration (mg mL⁻¹) – Concentration (mg mL⁻¹) of LZ in aqueous solution at pH 5.00.
• Absorbance (280 nm) – UV–Vis absorbance intensity of LZ measured at 280 nm
(dimensionless).
• Linear fit (y = 1.4315x) – Linear regression equation correlating absorbance with
concentration.
• R² – Coefficient of determination indicating the goodness of fit of the calibration curve.

Supplementary _Figure_S3b.x1sx: Description: This file gives the UV–Vis calibration curve related absorbance data for variable concentrations of lysozyme (LZ) at pH 10.7 measured at 280 nm wavelength.
Variables
• Concentration (mg mL⁻¹) – Concentration of LZ in aqueous solution at pH 10.7.
• Absorbance (280 nm) – UV–Vis absorbance intensity of LZ measured at 280 nm
(dimensionless).
• Linear fit (y = 1.5201x) – Linear regression equation correlating absorbance with
concentration.
• R² – Coefficient of determination indicating the goodness of fit of the calibration curve.

Supplementary _Figure_S4a.x1sx
Description: This file gives the UV–Vis calibration curve related absorbance data for variable concentrations of vancomycin (VC) at pH 5.00 measured at the wavelength of 280 nm.
Variables
• Concentration (mg mL⁻¹) – Concentration (mg mL⁻¹) of VC in aqueous solution at pH 5.0.
• Absorbance (280 nm) – UV–Vis absorbance intensity of VC measured at 280 nm
(dimensionless).
• Linear fit (y = 3.6764x) – Linear regression equation correlating absorbance with
concentration.
• R² – Coefficient of determination indicating the goodness of fit of the calibration curve.

Supplementary _Figure_S4b.x1sx
Description: This file gives the UV–Vis calibration curve related absorbance data for variable concentrations of vancomycin (VC) at pH 8.2 measured at the wavelength of 280 nm.
Variables
• Concentration (mg mL⁻¹) – Concentration of VC in aqueous solution at pH 8.2.
• Absorbance (280 nm) – UV–Vis absorbance intensity of VC measured at 280 nm
(dimensionless).
• Linear fit (y = 3.9901x) – Linear regression equation correlating absorbance with
concentration.
• R² – Coefficient of determination indicating the goodness of fit of the calibration curve.

Supplementary _Figure_S5.x1sx
Description: This file provides the UV–Vis absorbance values against different concentrations (mg mL⁻¹) of clonazepam (CZ) at pH 10.00 measured at the wavelength of 261 nm.
Variables
• Concentration (mg mL⁻¹) – Concentration of CZ in aqueous solution at pH 10.00.
• Absorbance (261 nm) – UV–Vis absorbance intensity of Clonazepam measured at 261 nm
(dimensionless).
• Linear fit (y =5.5958x) – Linear regression equation correlating absorbance with
concentration.
• R² – Coefficient of determination indicating the goodness of fit of the calibration curve.

Supplementary _Figure_S7a.x1sx
Description: This file provides the pore size data of mFe3O4 particles, mFe3O4/mSiO2 core-particles and mFe3O4/mSiO2/P(NIPAM-CEA-MBA) composite, calculated using the BJH method. The differential pore volume (dV(log r)) is given as a function of pore diameter.
Variables
• Pore diameter (Å) – Average pore diameter of the mesoporous materials expressed in
angstroms (Å).
• dV(log r) (cm³ g⁻¹) – Differential pore volume per logarithmic pore radius interval, normalized
by sample mass.

Supplementary _Figure_S7b.x1sx
Description: This file provides the nitrogen adsorption–desorption isotherm values for mFe3O4 particles, mFe3O4/mSiO2 core-particles and mFe3O4/mSiO2/P(NIPAM-CEA-MBA) composite measured as a function of relative pressure (P/P0).

Variables
• Relative pressure (P/P0) – Ratio of equilibrium pressure (P) to saturation vapor pressure (P0)
of nitrogen (dimensionless).
• Volume adsorbed (cc g⁻¹) – Volume of nitrogen (cc) adsorbed per gram of sample.

Supplementary _Figure_S8.x1sx
Description: This file gives the experimental values of sample weight loss as a function of increasing temperature obtained from TG analyses for mFe3O4 particles, mFe3O4/mSiO2 core-particles and mFe3O4/mSiO2/P(NIPAM-CEA-MBA) composite.
Variables
• Temperature (°C) – the heating temperature of the sample.
• Weight (%) – percentage of sample mass remaining during heating.

Code/software

Any program that will open a spreadsheet, such as Excel is recommended.

Access information

Other publicly accessible locations of the data: Not applicable

• None

Data was derived from the following sources: Not applicable

• None