Data from: Plant-produced viral nanoparticles decorated with nanobodies against HER2 improve retention and recruitment of immune cells in solid tumors
Data files
Mar 18, 2026 version files 68.32 KB
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EGFR_confocal.xlsx
8.88 KB
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EGFR_flow_cytometry.xlsx
8.90 KB
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HER2_confocal.xlsx
9.25 KB
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HER2_flow_cytometry.xlsx
8.91 KB
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PVX-nHER2-2_tumor.xlsx
9.18 KB
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PVX-VHH_size_characterization.xlsx
19.77 KB
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README.md
3.44 KB
Abstract
Potato virus X (PVX), a filamentous, positive-sense RNA plant virus, has been engineered into a molecular tool for diverse biotechnological applications, including cancer cell targeting. Here, we present the production and functional characterization of genetically-encoded PVX-derived nanoparticles decorated with nanobodies targeting two common receptors in human cancer cells, epidermal growth factor receptor (EGFR) and human epidermal growth factor 2 (HER2). We first generated a series of PVX-derived nanoparticles displaying distinct nanobodies against EGFR and HER2 in Nicotiana benthamiana plants. Self-assembly and structural integrity of the recombinant nanoparticles were confirmed by immunogold electron microscopy. We next characterized in vitro the cancer-cell binding capacity of the different recombinant viral nanoparticles (VNPs) by flow cytometry and confocal microscopy. Best performing VNPs were further assayed in vivo using tumor-bearing mice. Results showed that nanobody decoration can increase retention time and myeloid cell recruitment in the tumor microenvironment in HER2+ mouse tumor models in vivo. In conclusion, nanobody-displaying PVX-derived nanoparticles may constitute a new promising plant-produced biotechnological product for cancer immunotherapy.
Dataset DOI: 10.5061/dryad.x69p8czzn
This contains the data used for experimental analysis for the article: Plant-produced viral nanoparticles decorated with nanobodies against HER2 improve retention and recruitment of immune cells in solid tumors (Authors: Enrique Lozano-Sanchez#, Miguel A. Moreno-Gonzalez#, Fernando Merwaiss, Nicole F. Steinmetz, and José-Antonio Daròs).
These data support experimental efforts to engineer Potato virus X (PVX)–derived nanoparticles functionalized with nanobodies targeting EGFR and HER2 and to evaluate their structural integrity, cellular binding behavior, and biological performance. Data were generated from plant-based nanoparticle production, electron microscopy, in vitro cell-based assays, and in vivo studies in mouse tumor models.
Files and variables
File: EGFR_confocal.xlsx
Description: Dataset of total intensity values obtained from confocal images using PVX-nEGFR-1,2,3 and Anti-EGFR against EGFR+ cells (A-431). Images used for analysis can be found in Supporting Information of the main article.
Variables
- Particles are named in the top row, with total intensity values listed below.
File: EGFR_flow_cytometry.xlsx
Description: Dataset of mean fluorescence intensity values obtained via flow cytometry analysis using PVX-nEGFR-1,2,3 and Anti-EGFR against EGFR+ cells (A-431).
Variables
- Particles are named in the top row, with mean fluorescence intensity values listed below.
File: HER2_flow_cytometry.xlsx
Description: Dataset of mean fluorescence intensity values obtained via flow cytometry analysis using PVX-nHER2-1,2,3 and Anti-HER2 against HER2+ cells (CT26-HER2).
Variables
- Particles are named in the top row, with mean fluorescence intensity values listed below.
File: HER2_confocal.xlsx
Description: Dataset of total intensity values obtained from confocal images using PVX-nHER2-1,2,3 and Anti-EGFR against HER2+ cells (CT26-HER2). Images used for analysis can be found in Supporting Information of the main article.
Variables
- Particles are named in the top row, with total intensity values listed below.
File: PVX-nHER2-2_tumor.xlsx
Description: Dataset of total intensity values analyzing immune cell recruitment (Ly/6G-Ly-6G) and PVX-nHER2-2 presence (VNP) in SKBR3 tumor cryosections after intratumoral immunization. Images used for analysis can be found in Supporting Information of the main article.
Variables
- PBS (Phosphate-buffered saline); VNP (Viral nanoparticle)
File: PVX-VHH_size_characterization.xlsx
Description: Dataset of Potato virus X (PVX) and recombinant PVX displaying 3 different EGFR nanobodies (PVX-nEGFR-1,2 and 3) or HER2 nanobodies (PVX-nHER2-1,2 and 3) measurements (in nm) obtained using ImageJ software from different TEM images (images used can be found in Supporting Information of the main article).
Variables
- No variables were used in this dataset. Particles are named in the top row, with sample group sizes (in nanometers) listed below.
Code/software
All data files are provided in spreadsheet format (.xlsx) and can be accessed using Microsoft Excel or other compatible spreadsheet software.