Dataset DOI: 10.5061/dryad.xgxd254w6

Description of the data and file structure

Six horses underwent unilateral corneal photodynamic therapy with intrastromal infracyanine green and photoactivation with an 810nm diode laser (500mW for 2.5 min = 75 Joules). Complete ophthalmic examinations, clinical scoring, digital and infrared photography, ocular thermography, in vivo confocal microscopy (IVCM), and ultrasound biomicroscopy (UBM) were performed pre-treatment and post-treatment on study days 1, 5, 15, 33, and 103.

Clinical scoring parameters were based on a scoring system modified from the semiquantitative preclinical ocular toxicology scoring (SPOTS) system (Eaton et al, J Ocul Pharacol Ther. 2017). The treated eye of each horse underwent graded assessment of pupillary light reflexes, conjunctival hyperemia, chemosis, ocular discharge, corneal opacity size and severity, corneal vascularization, aqueous flare and cell, iris involvement, vitreal inflammation, lens involvement, fluorescein staining, and fundic abnormalities. Scores were numerated on a 0-2, 0-3, or a 0-4 scale depending on the parameter observed (Supplementary Table 1, Zenodo). Total clinical scores were calculated for each horse at each study timepoint. Total clinical scores for each horse at each study timepoint were compared using the Kruskal-Wallis test.

Files and variables

File: Clinical_Scores.csv

Description: This file provides the total clinical scores for each included horse at the different study time points. The clinical scoring system used can be viewed in the published manuscript (Supplementary Table 1, Zenodo). 

Variables

• Horse: Study animal number
• Day 1-pre: Data points acquired prior to corneal photodynamic therapy 
• Day 5: Data points acquired 5 days after corneal photodynamic therapy 
• Day 15: Data points acquired 15 days after corneal photodynamic therapy 
• Day 33: Data points acquired 33 days after corneal photodynamic therapy 
• Day 103: Data points acquired 103 days after corneal photodynamic therapy 

File: Corneal_Thermography.csv

Description: This file provides the corneal temperatures (in degrees Fahrenheit) for each included horse at the different study time points. Infrared thermography (IRT) images were taken using an infrared radiation camera (FLIR T1020 thermal camera). Images were taken at a distance of 3 feet from the eye with the region of interest (ROI) focused on the axial cornea. IRT images were collected in ambient lighting in the same temperature controlled room for all horses and all study time points. Corneal temperatures for each horse at each study timepoint were compared using the Friedman test.

Variables

• Horse: Study animal number
• Day 1 pre: Data points acquired prior to corneal photodynamic therapy 
• Day 1 post: Data points acquired immediately following corneal photodynamic therapy 
• Day 5: Data points acquired 5 days after corneal photodynamic therapy 
• Day 15: Data points acquired 15 days after corneal photodynamic therapy 
• Day 33: Data points acquired 33 days after corneal photodynamic therapy 
• Day 103: Data points acquired 103 days after corneal photodynamic therapy

File: Corneal_thickness_measurements_ultrasound_biomicroscopy.csv

Description: This file provides the mean axial corneal thickness measurements (in millimeters) for each included horse at the different study time points. Axial corneal thickness measurements were made using the ABSolu (Lumbirid® Medical Ophthalmology) software. Corneal thickness measurements were made from the outermost aspect of the corneal epithelium to the innermost aspect of the Descemet's membrane-endothelium complex at the maximum arch of the axial cornea. Post-treatment corneal thickness measurements were obtained in the axial cornea where the cornea was thickest. A total of 6 corneal thickness measurements were made from 6 unique UBM images for each eye at each study timepoint (3 with the marker oriented superiorly, and 3 with marker oriented nasally). The mean of these 6 measurements was utilized for statistical analysis. Mean axial corneal thickness measurements for each horse at each study timepoint were compared using the Friedman test.

Variables

• Horse: Study animal number
• Day 1 pre: Data points acquired prior to corneal photodynamic therapy 
• Day 1 post: Data points acquired immediately following corneal photodynamic therapy 
• Day 5: Data points acquired 5 days after corneal photodynamic therapy 
• Day 15: Data points acquired 15 days after corneal photodynamic therapy 
• Day 33: Data points acquired 33 days after corneal photodynamic therapy 
• Day 103: Data points acquired 103 days after corneal photodynamic therapy

Code/software

A spreadsheet software is required to view the csv files