https://doi.org/10.5061/dryad.xksn02vsg

Description of the data and file structure:

RNA gene expression profiles in MDA-MB-157 cells with or without BHLHE22 overexpression. The file contains the list of differentially expressed genes.

The inducible expression pLVX-TetOne-Puro vector was used to overexpress BHLHE22 in MDA-MB-157 cells. After cell infection, the infected cells were treated with doxycycline (dox, 1 μg/mL) to induce BHLHE22 overexpression. Then, the infected cells were underwent sequencing. The sequencing images were generated by the sequencing platform and converted into sequential data (reads) by CASAVA base recognition. The fastq file contains the sequence information of sequencing fragments and their corresponding sequencing quality information. The raw data from sequencing contained a small number of reads with sequencing connectors or with low sequencing quality. In order to ensure the quality and reliability of data analysis, the original data were filtered. After filtering out the joints and low-quality reads, clean reads were aligned to the human genome (GRCh38) using HISAT2 software. HTSeq (v2.0.2) transcriptome analysis quantitative software was used for the reads count. The FPKM of each gene was then calculated based on the length of the gene and the readings mapped to that gene were calculated. DESeq2 software was used to perform differential expression analysis between different groups. P <0.05 & |log2 (foldchange)| ≥1 was set as the threshold for significant differential expression.

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