This readme file was generated on [2024-02-01] by [K Chandrakumara]

GENERAL INFORMATION

Title of Dataset: Data from: Aphid-induced phytochemicals in Brassica juncea (L.) Czern & Coss. afflicting host preference and bionomics of Lipaphis erysimi (Kaltenbach)

Author/Principal Investigator Information

Name: Dr Mukesh K Dhillon

ORCID: https://orcid.org/0000-0001-6781-9211

Institution: ICAR-Indian Agricultural Research Institute, New Delhi, India

Address: ICAR-Indian Agricultural Research Institute, New Delhi, India-110012

Email: mukeshdhillon@rediffmail.com

Date of data collection: 2021-01-15 to 2022-05-15

Geographic location of data collection: ICAR-Indian Agricultural Research Institute, New Delhi (28°38′30.5″N, 77°09′58.2″E, 228 m AMSL)

Information about funding sources that supported the collection of the data: Nil

SHARING/ACCESS INFORMATION

Licenses/restrictions placed on the data: Nil

Links to publications that cite or use the data: DOI:

Links to other publicly accessible locations of the data: Nil

Links/relationships to ancillary data sets: Nil

Was data derived from another source?

If yes, list source(s): Nil

Recommended citation for this dataset: Chandrakumara et al., 2024, Data from: Aphid-induced phytochemicals in Brassica juncea (L.) Czern & Coss. afflicting host preference and bionomics of Lipaphis erysimi (Kaltenbach), Dryad, Dataset

DATA & FILE OVERVIEW

File List: Biochemicalsfinal, have the values of different biochemical parameters in 23 mustard cultivars for both constitutive and induced defense and per cent change over the constitutive levels, Biologyfinal, contains the values of different biological parameters of Lipaphis erysimi on siliquae of 23 mustard cultivars, Bionomicsfinal, contains the values of different life table parameters of Lipaphis erysimi on siliquae of 23 mustard cultivars, Siliquaefinal, contains the values of siliquae preference among the 23 mustard cultivars by the Lipaphis erysimi.

Relationship between files, if important: The aphid-induced phytochemicals significantly distressed the host preference, developmental biology and the life table parameters of Lipaphis erysimi.

Additional related data collected that was not included in the current data package: Nil

Are there multiple versions of the dataset?

If yes, name of file(s) that was updated: Nil

Why was the file updated?

When was the file updated?

METHODOLOGICAL INFORMATION

Description of methods used for estimation of different biochemical parameters: After 48 h of exposure, the siliquae of different mustard cultivars were collected from L. erysimi infested and those plants free from aphid infestation, and brought to the laboratory for the estimation of nutritional, antinutritional, photosynthetic pigments and various enzymes. Aphids were removed from samples infested with them using a camel hair brush before the samples were processed for biochemical analysis. Test cultivars' two-gram tissues were separately crushed in liquid nitrogen and mixed with 10 millilitres of a 50 mM phosphate buffer (pH 7.8). After transferring to centrifuge tubes, the slurry was centrifuged for 20 minutes at 4 °C at 12000 rpm. The supernatant was gathered and stored in a deep freezer at -20 °C in order to estimate the previously mentioned biochemical components. For photosynthetic pigments, total glucosinolates and myrosinase estimation, directly fresh tissues were used instead of supernatant. There were three replications for each test cultivars and biochemical constituent in a CRD. Total sugars and proteins were assessed using the methods given by Dubois et al. (1956) and Bradford (1976), respectively, and expressed in mg/g of plant tissue. The methods given by Prieto et al. (1999), Amorim et al. (2008), Singleton and Rossi (1965) and Benzie and Strain (1999) were used to estimate total antioxidant, total tannins, total phenols and ferric ion reducing antioxidant power (FRAP), and the values obtained were expressed in mg/g of plant tissue. The total glucosinolates content was estimated by means of the protocol given by Kraling et al. (1990), and values obtained were expressed in μmol /g of tissue. Further, total carotenoids and chlorophyll- a, b and total chlorophyll were estimated by following the method given by Nayek et al. (2014). According to the techniques provided by Diallinas et al. (1997), Ali et al. (2005), Aebi (1984), Fritz et al. (1976), Thorpe and Beaudoin-Eagan (1985), and Piekarska et al. (2013), the activities of ascorbate oxidase (AO), ascorbate peroxidase (APX), catalase, phenylalanine ammonia lyase (PAL), tyrosine ammonia lyase (TAL), and myrosinase were determined.

Description of methods used for biological parameters: Under carefully controlled laboratory conditions, the biological studies of L. erysimi on test cultivar siliquae were carried out at 15±3 oC temperature, 60–70% relative humidity, and 12L: 12D photoperiod. Aphids were gathered from the field and raised in a lab setting on mustard siliquae in glass Petri dishes measuring 10 cm in diameter and 2 cm in height. Using a fine, moist camel hair brush, newly hatched nymphs from laboratory-raised aphids were gathered and moved to siliquae of each test cultivar. The fresh siliquae of respective genotype were provided every 24 h till the completion of biological studies on the test genotypes. Each test cultivar in the experiment had 15 replications in a CRD. Total nymphal period (TNP), pre-reproductive period (PRP), reproductive period (RP), post-reproductive period (PRP), total developmental period (TDP), and daily fecundity were the subjects of the observations. Additionally, the total number of progenies produced was noted, and the percentage of nymphs that survived after 48 hours of emergence was calculated and reported as survival (%) per female.

Description of methods used for bionomic parameters: The life table of L. erysimi was constructed by using ‘TWOSEX-MS Chart’ software. According to the age-stage, two-sex life table principle (Chi, 1988; Huang and Chi, 2012) and method (Tuan et al., 2014), the parameters like intrinsic rate of increase (r), finite rate of increase (λ), net reproductive rate (R0), gross reproductive rate (GRR), mean generation time (T) and doubling time (DT) were calculated.

Description of methods used for host preference:In a completely randomised design (CRD), 15 randomly selected plants were used to create 15 replications of the siliquae from each cultivar's main branch. A single siliqua of every variety was arranged in a circular pattern on blotting paper, spaced two centimetres apart, with 230 aphids released in the centre. The siliquae were then entirely covered with a mosquito net measuring 95 × 55 × 40 cm, and the temperature was maintained at 15±3 °C. Aphids were counted on each siliqua after a 24 h period, and the results were expressed as a percentage of nymphs found on the corresponding cultivar's siliquae.

Instrument- or software-specific information needed to interpret the data: Nil.

Standards and calibration information, if appropriate: pH meter was calibrated before recording of data using approved standards.

Environmental/experimental conditions: Twenty-three B. juncea cultivars were grown in 5 row plots (5 m length), with 30 × 15 cm spacing in experimental plots of the Division of Entomology, Indian Agricultural Research Institute, New Delhi during the 2021 and 2022 winter cropping seasons. To raise the crop, all advised agronomic approaches were used, with the exception of insecticidal application.

Describe any quality-assurance procedures performed on the data: Nil

People involved with sample collection, processing, analysis and/or submission: K. Chandrakumara, Mukesh K. Dhillon, Naveen Singh.

DATA-SPECIFIC INFORMATION FOR: [Biochemicalsfinal]

Number of variables: 51 (17 variables in the multiple of 3 conditions namely, constitutive, induced and per cent change)

Number of cases/rows: 70

The row names in the first column refers to the name of diverse mustard cultivars used in the study which are replicated thrice.

• Variable List: Constitutive_Protein, Induced_protein, Percentchange_protein, Constitutive_Sugars, Induced_Sugars, Percentchange_Sugars, Constitutive_Phenols, Induced_Phenols, Percentchange_Phenols, Constitutive_Tannins, Induced_Tannins, Percentchange_Tannins, Constitutive_Antioxidants, Induced_Antioxidants, Percentchange_Antioxidants, Constitutive_FRAP, Induced_FRAP, Percentchange_FRAP, Constitutive_Glucosinolates, Induced_Glucosinolates, Percentchange_Glucosinolates, Constitutive_ChlA, Induced_ChlA, Percentchange_ChlA, Constitutive_ChlB, Induced_ChlB, Percentchange_ChlB, Constitutive_TotalChl, Induced_TotalChl, Percentchange_TotalChl, Constitutive_Carotenoids, Induced_Carotenoids, Percentchange_Carotenoids, Constitutive_AO, Induced_AO, Percentchange_AO, Constitutive_APX, Induced_APX, Percentchange_APX, Constitutive_Catalase, Induced_Catalase, Percentchange_Catalase, Constitutive_PAL, Induced_PAL, Percentchange_PAL, Constitutive_TAL, Induced_TAL, Percentchange_TAL, Constitutive_Myrosinase, Induced_Myrosinase, Percentchange_Myrosinase values with 3 replications

• Abbreviations used in varibale list: FRAP- Ferric ion Reducing Antioxidant Power, ChlA- Chlorophyll A, ChlB- Chlorophyll B, TotalChl- Total chlorophyll, AO- Ascorbate oxidase, Ascorbate Peroxidase, PAL- Phenylalanine ammonia lyase, TAL- Tyrosine ammonia lyase

• Description: Constitutive variable refers to the amount of respective phytochemical in the healthy tissue/ undamaged plant tissue; Induced variable refers to the amount of phytochemicals produced in response to aphid damage; Percentchange varibale refers to the per cent change in the induced levels of phytochemicals over the constitutive levels of that particular phytochemicals.

• Units of measurement: Constitutive_Protein, Induced_protein, Constitutive_Sugars, Induced_Sugars, Constitutive_Phenols, Induced_Phenols, Constitutive_Tannins, Induced_Tannins, Constitutive_Antioxidants, Induced_Antioxidants, Constitutive_FRAP and Induced_FRAP were expressed in mg/g of plant tissue, Constitutive_Glucosinolates and Induced_Glucosinolates were expressed in μmol/g, Constitutive_ChlA, Induced_ChlA, Constitutive_ChlB, Induced_ChlB, Constitutive_TotalChl, Induced_TotalChl, Constitutive_Carotenoids and Induced_Carotenoids were expressed in μg/ml, Constitutive_AO, Induced_AO, Constitutive_APX, Induced_APX, Constitutive_Catalase, Induced_Catalase, Constitutive_PAL, Induced_PAL, Constitutive_TAL, Induced_TAL, Constitutive_Myrosinase and Induced_Myrosinase were expressed in U/ml, and Percentchange_protein, Percentchange_Sugars, Percentchange_Phenols, Percentchange_Tannins, Percentchange_Antioxidants, Percentchange_FRAP, Percentchange_Glucosinolates, Percentchange_ChlA, Percentchange_ChlB, Percentchange_TotalChl, Percentchange_Carotenoids, Percentchange_AO, Percentchange_APX, Percentchange_Catalase, Percentchange_PAL, Percentchange_TAL, Percentchange_Myrosinase were expressed in percent values.

DATA-SPECIFIC INFORMATION FOR: [Biologyfinal]

Number of variables: 10

Number of cases/rows: 346

The row names in the first column refers to the name of diverse mustard cultivars used in the study which are in 15 replication.

• Variable List: Firstinstar, Secondinstar, thirdinstar, Fourthinstar, Totalnymphalperiod, Prereproductive, Reproductive, Postreproductive, fecundity and nymphssurvived values of L. erysimi on 23 mustard cultivars with 15 replications.

• Description: Firstinstar- birth of first instar to first moult; Secondinstar- starting from second instar to second moult; thirdinstar- starting from third instar to third moult; Fourthinstar- starting from fourth instar to fourth moult; Totalnymphalperiod- birth of first instar to end of fourth instar; Prereproductive- Starting from adult to first laying; reproductive- birth of first nymph to last nymph; postreprodutive- birth of last nymph to death of respective adult female; totaldevelopmentalperiod- birth of the nymph to death of resulting female; fecundity- number of nymphs produced by each female; the total offsprings produced were observed and survival of nymphs was also calculated after 48 h of emergence and expressed as survival (%) per female.

• Units: Firstinstar, Secondinstar, thirdinstar, Fourthinstar, Totalnymphalperiod, Prereproductive, Reproductive and Postreproductive were expressed in hours (h), fecundity was expressed in number of nymphs produced per female, and nymphssurived expressed in number of nymphs surived.

DATA-SPECIFIC INFORMATION FOR: [Bionomicsfinal]

Number of variables: 6

Number of cases/rows: 691

The row names in the first column refers to the name of diverse mustard cultivars used in the study which are in 30 replication.

• Variable List: Intrinsicrateofincrease, finiterateofincrease, Netreproductiverate, Grossreproductiverate, Meangenerationtime and Doublingtime values of L. erysimi on 23 mustard cultivars with 30 replications.

• Description: Intrinsic rate of increase (r): The population size will increase at the rate of er per time unit; Finite rate of increase (λ): The population size will increase at the rate of λ per time unit; Net reproductive rate (R0): Number of female offspring produced per female in a population per generation; Gross reproductive rate (GRR): Total lifetime reproduction in the absence of mortality; Mean generation time (T): Interval between birth of an individual and birth of its offspring; Doubling time (DT): Time it takes for a population to double in size/value.

• Units: Intrinsic rate of increase and Finite rate of increase were expressed in d-1 (per day), Net reproductive rate and Gross reproductive rate were expressed in offsprings per female, and Mean generation time and Doubling time were expressed in days.

DATA-SPECIFIC INFORMATION FOR: [Siliquaefinal]

Number of variables: 2

Number of cases/rows: 346

The row names in the first column refers to the name of diverse mustard cultivars used in the study which are in 15 replication.

• Variable List: nymphssettled and Percentrecovered on 23 mustard cultivars with 15 replications.

• Units: nymphssettled was expressed in number of nymphs settled; percentrecovered expressed in percent values.

Missing data codes: Nil

Specialized formats or other abbreviations used: Nil