Data from: Differential regulation of p62-ubiquitin conjugates in neurons versus astrocytes during cellular stress
Data files
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README.md
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Sidibe_et_al.zip
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Abstract
This dataset includes quantification of levels of p62, ubiquitin, LC3, p62-ubiquitin colocalization, and/or p62-GFP(LC3) colocalization in neurons or astrocytes under the following paradigms: (1) Lysosomal damage induced with LLOMe treatment (Fig. 1E-G’, S2A in associated manuscript), (2) Pharmacological reduction of ubiquitination with TAK-243 prior to LLOMe treatment (Fig. 2C-D’), (3) 30-minute starvation in Earle’s Balanced Salt Solution (EBSS; Fig. 3B-D’; S2B-C), (4) 4-hour starvation in EBSS (Fig. S2D-E, S3), (5) Pharmacological reduction of ubiquitination with TAK-243 prior to 30 minutes of EBSS starvation (Fig. 4C-D’, S2F-G, S4). Data are provided as .csv files for all figures for broad accessibility. Files are organized by figure number. All experimental data were generated using cocultures of primary mouse astrocytes and neurons; procedures were performed under IACUC-approved protocols. No restrictions apply to data reuse. These data enable independent verification of statistical analyses.
Dataset DOI: 10.5061/dryad.z612jm6s2
DATASET FILES: Sidibe_et_al.zip
DESCRIPTION OF THE DATA AND FILE STRUCTURE
GENERAL INFORMATION
- This dataset contains the numerical values underlying all main figures (Figs. 1-4) and supplementary figures (Figs. S2-S4) in Sidibe et al., "Differential regulation of p62-ubiquitin conjugates in neurons versus astrocytes during cellular stress", PLoS ONE.
- File naming convention:
- Files are organized into folders that are titled with the relevant Figure number
- Files are named by figure panel (e.g., Figure 1E_Neurons_p62_biological replicates.csv). The prefix indicates the figure number and panel, followed by the cell type that is measured (e.g., neurons or astrocytes), followed by the marker that is measured (e.g., p62, ubiquitin, p62-Ub Colocalization, GFP(LC3), p62-GFP(LC3) Colocalization, p62 puncta count, or p62 puncta size), followed by replicate identification (e.g., technical replicates or biological replicates).
- File formats: Data are provided as .csv files for all figures for broad accessibility.
- Columns: Each column indicates the treatment
- Rows: Each row represents an individual measurement (either a technical replicate or a biological replicate, as indicated)
GLOSSARY OF KEY TERMS
- Ub: Ubiquitin
- LLOMe: L-Leucyl-L-Leucine methyl ester (agent to damage lysosomes)
- EBSS: Earle’s Balanced Salt Solution (starvation medium)
- TAK-243: inhibitor of the E1 ubiquitin-activating enzyme
FILE-BY-FILE DESCRIPTIONS
FIGURE 1
Figure 1E_Neurons_p62_biological replicates.csv
- Description: The effect of LLOMe treatment on p62 puncta area in the soma of neurons
- Columns: DMSO (solvent control) versus LLOMe (1 mM) for 1 hour
- Values: % p62 puncta area normalized to soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 1E_Neurons_p62_technical replicates.csv
- Description: The effect of LLOMe treatment on p62 puncta area in the soma of neurons
- Columns: DMSO (solvent control) versus LLOMe (1 mM) for 1 hour
- Values: % p62 puncta area normalized to soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 1E’_Astrocytes_p62_biological replicates.csv
- Description: The effect of LLOMe treatment on p62 puncta area in the soma of astrocytes
- Columns: DMSO (solvent control) versus LLOMe (1 mM) for 1 hour
- Values: % p62 puncta area normalized to soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 1E’_Astrocytes_p62_technical replicates.csv
- Description: The effect of LLOMe treatment on p62 puncta area in the soma of astrocytes
- Columns: DMSO (solvent control) versus LLOMe (1 mM) for 1 hour
- Values: % p62 puncta area normalized to soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 1F_Neurons_Ubiquitin_biological replicates.csv
- Description: The effect of LLOMe treatment on Ubiquitin puncta area in the soma of neurons
- Columns: DMSO (solvent control) versus LLOMe (1 mM) for 1 hour
- Values: % Ub puncta area normalized to soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 1F_Neurons_Ubiquitin_technical replicates.csv
- Description: The effect of LLOMe treatment on Ubiquitin puncta area in the soma of neurons
- Columns: DMSO (solvent control) versus LLOMe (1 mM) for 1 hour
- Values: % Ub puncta area normalized to soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 1F’_Astrocytes_Ubiquitin_biological replicates.csv
- Description: The effect of LLOMe treatment on Ubiquitin puncta area in the soma of astrocytes
- Columns: DMSO (solvent control) versus LLOMe (1 mM) for 1 hour
- Values: % Ub puncta area normalized to soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 1F’_Astrocytes_Ubiquitin_technical replicates.csv
- Description: The effect of LLOMe treatment on Ubiquitin puncta area in the soma of astrocytes
- Columns: DMSO (solvent control) versus LLOMe (1 mM) for 1 hour
- Values: % Ub puncta area normalized to soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 1G_Neurons_p62-Ub Colocalization_biological replicates.csv
- Description: The effect of LLOMe treatment on colocalization area between p62 and Ubiquitin-positive puncta in the soma of neurons
- Columns: DMSO (solvent control) versus LLOMe (1 mM) for 1 hour
- Values: % p62-Ub colocalization area normalized to soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 1G_Neurons_p62-Ub Colocalization_technical replicates.csv
- Description: The effect of LLOMe treatment on colocalization area between p62 and Ubiquitin-positive puncta in the soma of neurons
- Columns: DMSO (solvent control) versus LLOMe (1 mM) for 1 hour
- Values: % p62-Ub colocalization area normalized to soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 1G’_Astrocytes_p62-Ub Colocalization_biological replicates.csv
- Description: The effect of LLOMe treatment on colocalization area between p62 and Ubiquitin-positive puncta in the soma of astrocytes
- Columns: DMSO (solvent control) versus LLOMe (1 mM) for 1 hour
- Values: % p62-Ub colocalization area normalized to soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 1G’_Astrocytes_p62-Ub Colocalization_technical replicates.csv
- Description: The effect of LLOMe treatment on colocalization area between p62 and Ubiquitin-positive puncta in the soma of astrocytes
- Columns: DMSO (solvent control) versus LLOMe (1 mM) for 1 hour
- Values: % p62-Ub colocalization area normalized to soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
FIGURE 2
Figure 2C_Neurons_p62_biological replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to LLOMe-induced lysosomal damage in the soma of neurons
- Columns: DMSO pretreatment + LLOMe versus TAK-243 pretreatment + LLOMe treatment
- Values: % p62 puncta area normalized to soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 2C_Neurons_p62_technical replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to LLOMe-induced lysosomal damage in the soma of neurons
- Columns: DMSO pretreatment + LLOMe versus TAK-243 pretreatment + LLOMe treatment
- Values: % p62 puncta area normalized to soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 2C’_Astrocytes_p62_biological replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to LLOMe-induced lysosomal damage in the soma of astrocytes
- Columns: DMSO pretreatment + LLOMe versus TAK-243 pretreatment + LLOMe treatment
- Values: % p62 puncta area normalized to soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 2C’_Astrocytes_p62_technical replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to LLOMe-induced lysosomal damage in the soma of astrocytes
- Columns: DMSO pretreatment + LLOMe versus TAK-243 pretreatment + LLOMe treatment
- Values: % p62 puncta area normalized to soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 2D_Neurons_Ubiquitin_biological replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to LLOMe-induced lysosomal damage in the soma of neurons
- Columns: DMSO pretreatment + LLOMe versus TAK-243 pretreatment + LLOMe treatment
- Values: Soma ubiquitin mean intensity normalized to DMSO control
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 2D_Neurons_Ubiquitin_technical replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to LLOMe-induced lysosomal damage in the soma of neurons
- Columns: DMSO pretreatment + LLOMe versus TAK-243 pretreatment + LLOMe treatment
- Values: Soma ubiquitin mean intensity normalized to DMSO control
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 2D’_Astrocytes_Ubiquitin_biological replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to LLOMe-induced lysosomal damage in the soma of astrocytes
- Columns: DMSO pretreatment + LLOMe versus TAK-243 pretreatment + LLOMe treatment
- Values: Soma ubiquitin mean intensity normalized to DMSO control
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 2D’_Astrocytes_Ubiquitin_technical replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to LLOMe-induced lysosomal damage in the soma of astrocytes
- Columns: DMSO pretreatment + LLOMe versus TAK-243 pretreatment + LLOMe treatment
- Values: Soma ubiquitin mean intensity normalized to DMSO control
- Rows: Measurements from individual cells (i.e., the technical replicates)
FIGURE 3
Figure 3B_Neurons_p62_biological replicates.csv
- Description: The effect of 30 minutes of EBSS on p62 puncta area in the soma (nuclear subtracted) of neurons
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % p62 puncta area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 3B_Neurons_p62_technical replicates.csv
- Description: The effect of 30 minutes of EBSS on p62 puncta area in the soma (nuclear subtracted) of neurons
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % p62 puncta area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 3B’_Astrocytes_p62_biological replicates.csv
- Description: The effect of 30 minutes of EBSS on p62 puncta area in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % p62 puncta area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 3B’_Astrocytes_p62_technical replicates.csv
- Description: The effect of 30 minutes of EBSS on p62 puncta area in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % p62 puncta area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 3C_Neurons_Ubiquitin_biological replicates.csv
- Description: The effect of 30 minutes of EBSS on Ub puncta area in the soma (nuclear subtracted) of neurons
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % Ub puncta area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 3C_Neurons_Ubiquitin_technical replicates.csv
- Description: The effect of 30 minutes of EBSS on Ub puncta area in the soma (nuclear subtracted) of neurons
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % Ub puncta area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 3C’_Astrocytes_Ubiquitin_biological replicates.csv
- Description: The effect of 30 minutes of EBSS on Ub puncta area in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % Ub puncta area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 3C’_Astrocytes_Ubiquitin_technical replicates.csv
- Description: The effect of 30 minutes of EBSS on Ub puncta area in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % Ub puncta area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 3D_Neurons_p62-Ub Colocalization_biological replicates.csv
- Description: The effect of 30 minutes of EBSS on colocalization area between p62 and Ubiquitin-positive puncta in the soma (nuclear subtracted) of neurons
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % p62-Ub colocalization area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 3D_Neurons_p62-Ub Colocalization_technical replicates.csv
- Description: The effect of 30 minutes of EBSS on colocalization area between p62 and Ubiquitin-positive puncta in the soma (nuclear subtracted) of neurons
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % p62-Ub colocalization area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 3D’_Astrocytes_p62-Ub Colocalization_biological replicates.csv
- Description: The effect of 30 minutes of EBSS on colocalization area between p62 and Ubiquitin-positive puncta in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % p62-Ub colocalization area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 3D’_Astrocytes_p62-Ub Colocalization_technical replicates.csv
- Description: The effect of 30 minutes of EBSS on colocalization area between p62 and Ubiquitin-positive puncta in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % p62-Ub colocalization area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
FIGURE 4
Figure 4C_Neurons_p62_biological replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of neurons
- Columns: DMSO pretreatment + EBSS media versus TAK-243 pretreatment + EBSS media
- Values: % p62 puncta area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 4C_Neurons_p62_technical replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of neurons
- Columns: DMSO pretreatment + EBSS media versus TAK-243 pretreatment + EBSS media
- Values: % p62 puncta area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 4C’_Astrocytes_p62_biological replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of astrocytes
- Columns: DMSO pretreatment + EBSS media versus TAK-243 pretreatment + EBSS media
- Values: % p62 puncta area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 4C’_Astrocytes_p62_technical replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of astrocytes
- Columns: DMSO pretreatment + EBSS media versus TAK-243 pretreatment + EBSS media
- Values: % p62 puncta area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 4D_Neurons_Ubiquitin_biological replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of neurons
- Columns: DMSO pretreatment + EBSS media versus TAK-243 pretreatment + EBSS media
- Values: % Ub puncta area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 4D_Neurons_Ubiquitin_technical replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of neurons
- Columns: DMSO pretreatment + EBSS media versus TAK-243 pretreatment + EBSS media
- Values: % Ub puncta area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure 4D’_Astrocytes_Ubiquitin_biological replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of astrocytes
- Columns: DMSO pretreatment + EBSS media versus TAK-243 pretreatment + EBSS media
- Values: % Ub puncta area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure 4D’_Astrocytes_Ubiquitin_technical replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of astrocytes
- Columns: DMSO pretreatment + EBSS media versus TAK-243 pretreatment + EBSS media
- Values: % Ub puncta area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
FIGURE S2
Figure S2A_Astrocytes_GFP(LC3)_biological replicates.csv
- Description: The effect of LLOMe treatment on GFP(LC3) puncta area in the soma of astrocytes
- Columns: DMSO (solvent control) versus LLOMe (1 mM) for 1 hour
- Values: % GFP(LC3) puncta area normalized to soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure S2A_Astrocytes_GFP(LC3)_technical replicates.csv
- Description: The effect of LLOMe treatment on GFP(LC3) puncta area in the soma of astrocytes
- Columns: DMSO (solvent control) versus LLOMe (1 mM) for 1 hour
- Values: % GFP(LC3) puncta area normalized to soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure S2B_Astrocytes_GFP(LC3)_biological replicates.csv
- Description: The effect of 30 minutes of EBSS on GFP(LC3) puncta area in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % GFP(LC3) puncta area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure S2B_Astrocytes_GFP(LC3)_technical replicates.csv
- Description: The effect of 30 minutes of EBSS on GFP(LC3) puncta area in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % GFP(LC3) puncta area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure S2C_Astrocytes_p62-GFP(LC3) Colocalization_biological replicates.csv
- Description: The effect of 30 minutes of EBSS on colocalization area between p62 and GFP(LC3)-positive puncta in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % p62-GFP(LC3) colocalization area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure S2C_Astrocytes_p62-GFP(LC3) Colocalization_technical replicates.csv
- Description: The effect of 30 minutes of EBSS on colocalization area between p62 and GFP(LC3)-positive puncta in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 30 minutes
- Values: % p62-GFP(LC3) colocalization area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure S2D_Astrocytes_GFP(LC3)_biological replicates.csv
- Description: The effect of 4 hours of EBSS on GFP(LC3) puncta area in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % GFP(LC3) puncta area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure S2D_Astrocytes_GFP(LC3)_technical replicates.csv
- Description: The effect of 4 hours of EBSS on GFP(LC3) puncta area in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % GFP(LC3) puncta area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure S2E_Astrocytes_p62-GFP(LC3) Colocalization_biological replicates.csv
- Description: The effect of 4 hours of EBSS on colocalization area between p62 and GFP(LC3)-positive puncta in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % p62-GFP(LC3) colocalization area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure S2E_Astrocytes_p62-GFP(LC3) Colocalization_technical replicates.csv
- Description: The effect of 4 hours of EBSS on colocalization area between p62 and GFP(LC3)-positive puncta in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % p62-GFP(LC3) colocalization area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure S2F_Astrocytes_GFP(LC3)_biological replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of astrocytes
- Columns: DMSO pretreatment + EBSS media versus TAK-243 pretreatment + EBSS media
- Values: % GFP(LC3) puncta area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure S2F_Astrocytes_GFP(LC3)_technical replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of astrocytes
- Columns: DMSO pretreatment + EBSS media versus TAK-243 pretreatment + EBSS media
- Values: % GFP(LC3) puncta area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure S2G_Astrocytes_p62-GFP(LC3) Colocalization_biological replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of astrocytes
- Columns: DMSO pretreatment + EBSS media versus TAK-243 pretreatment + EBSS media (30 min)
- Values: % p62 puncta colocalized with GFP(LC3) puncta, normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure S2G_Astrocytes_p62-GFP(LC3) Colocalization_technical replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of astrocytes
- Columns: DMSO pretreatment + EBSS media versus TAK-243 pretreatment + EBSS media (30 min)
- Values: % p62 puncta colocalized with GFP(LC3) puncta, normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
FIGURE S3
Figure S3B_Neurons_p62_biological replicates.csv
- Description: The effect of 4 hours of EBSS on p62 puncta area in the soma (nuclear subtracted) of neurons
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % p62 puncta area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure S3B_Neurons_p62_technical replicates.csv
- Description: The effect of 4 hours of EBSS on p62 puncta area in the soma (nuclear subtracted) of neurons
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % p62 puncta area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure S3B’_Astrocytes_p62_biological replicates.csv
- Description: The effect of 4 hours of EBSS on p62 puncta area in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % p62 puncta area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure S3B’_Astrocytes_p62_technical replicates.csv
- Description: The effect of 4 hours of EBSS on p62 puncta area in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % p62 puncta area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure S3C_Neurons_Ubiquitin_biological replicates.csv
- Description: The effect of 4 hours of EBSS on Ub puncta area in the soma (nuclear subtracted) of neurons
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % Ub puncta area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure S3C_Neurons_Ubiquitin_technical replicates.csv
- Description: The effect of 4 hours of EBSS on Ub puncta area in the soma (nuclear subtracted) of neurons
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % Ub puncta area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure S3C’_Astrocytes_Ubiquitin_biological replicates.csv
- Description: The effect of 4 hours of EBSS on Ub puncta area in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % Ub puncta area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure S3C’_Astrocytes_Ubiquitin_technical replicates.csv
- Description: The effect of 4 hours of EBSS on Ub puncta area in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % Ub puncta area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure S3D_Neurons_p62-Ub Colocalization_biological replicates.csv
- Description: The effect of 4 hours of EBSS on colocalization area between p62 and Ubiquitin-positive puncta in the soma (nuclear subtracted) of neurons
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % p62-Ub colocalization area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure S3D_Neurons_p62-Ub Colocalization_technical replicates.csv
- Description: The effect of 4 hours of EBSS on colocalization area between p62 and Ubiquitin-positive puncta in the soma (nuclear subtracted) of neurons
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % p62-Ub colocalization area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure S3D’_Astrocytes_p62-Ub Colocalization_biological replicates.csv
- Description: The effect of 4 hours of EBSS on colocalization area between p62 and Ubiquitin-positive puncta in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % p62-Ub colocalization area normalized to cytoplasmic soma area
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure S3D’_Astrocytes_p62-Ub Colocalization_technical replicates.csv
- Description: The effect of 4 hours of EBSS on colocalization area between p62 and Ubiquitin-positive puncta in the soma (nuclear subtracted) of astrocytes
- Columns: Fed conditions (control) versus EBSS (starvation) for 4 hours
- Values: % p62-Ub colocalization area normalized to cytoplasmic soma area
- Rows: Measurements from individual cells (i.e., the technical replicates)
FIGURE S4
Figure S4A_Astrocytes_p62 puncta count_biological replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of astrocytes
- Columns: DMSO pretreatment + Fed media versus TAK-243 pretreatment + EBSS media (30 min)
- Values: p62 puncta count per cell
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure S4A_Astrocytes_p62 puncta count_technical replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of astrocytes
- Columns: DMSO pretreatment + Fed media versus TAK-243 pretreatment + EBSS media (30 min)
- Values: p62 puncta count per cell
- Rows: Measurements from individual cells (i.e., the technical replicates)
Figure S4B_Astrocytes_p62 puncta size_biological replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of astrocytes
- Columns: DMSO pretreatment + Fed media versus TAK-243 pretreatment + EBSS media (30 min)
- Values: Mean p62 puncta size (pixels2) per cell (total puncta area/puncta number)
- Rows: Biological means from each of the independent experiments (i.e., the biological replicates)
Figure S4B_Astrocytes_p62 puncta size_technical replicates.csv
- Description: The effect of reducing ubiquitination with TAK-243 pretreatment on the response to EBSS (30 min) in the soma (nuclear subtracted) of astrocytes
- Columns: DMSO pretreatment + Fed media versus TAK-243 pretreatment + EBSS media (30 min)
- Values: Mean p62 puncta size (pixels2) per cell (total puncta area/puncta number)
- Rows: Measurements from individual cells (i.e., the technical replicates)
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