Data from: An antigen panel to assess the regional relevance of foot and mouth disease vaccines
Data files
May 15, 2025 version files 170.44 KB
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README.md
3.88 KB
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SD1_mAb_info.xlsx
53.82 KB
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SD2_pAb_info.xlsx
20.91 KB
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SD3_mAb_data.xlsx
24.47 KB
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SD4_pAb_data.xlsx
24.98 KB
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SD5_VNT_data.xlsx
42.37 KB
May 20, 2025 version files 172.43 KB
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README.md
4.12 KB
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SD1_mAb_info.xlsx
53.77 KB
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SD2_pAb_info.xlsx
20.92 KB
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SD3_mAb_data.xlsx
25.16 KB
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SD4_pAb_data.xlsx
25.64 KB
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SD5_VNT_data.xlsx
42.83 KB
Abstract
Despite widespread use of inactivated vaccines to control foot-and-mouth disease (FMD), there is no systematic approach to demonstrate the regional relevance of these products against the specific serotypes and strains that circulate in endemic countries in Africa and Asia. Failure to adopt independent testing of FMD vaccines has contributed to poor trust in their quality and a lack of investment in vaccination programmes. Therefore, a reference antigen panel representing four serotypes, tailored for East Africa, has been established and used to measure FMDV-specific antibody responses in cattle after administration of FMD vaccines commercially available in the region. This revealed inconsistencies and gaps in cross-neutralisation responses that are evident for some vaccines even after giving booster doses. It is concluded that the East Africa reference antigen panel can be used to evaluate FMD vaccine potency and drive up vaccine quality. Further panels could be developed and deployed for other endemic regions.
Description
Supplementary Data 1 (SD1_mAb_info.xlsx file)
Characteristics of foot-and-mouth disease virus (FMDV) monoclonal antibody (mAb) used for antigenic typing - Murine mAbs used for antigenic typing had been prepared over many years at the WOAH FMD Reference Laboratory in Brescia, Italy (Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini") and included both neutralising and non-neutralising Mabs. mAbs were raised against:
- FMDV serotype O O/Switzerland/1965 or O Manisa lineages (O MAN & O-1 and O UK 2001 spreadsheets);
- different FMDV serotype A strains (Various Type A spreadsheet);
- FMDV serotype SAT 1 SAT1/BOT 1/68 lineage (SAT1 BOT 1-68 spreadsheet);
- FMDV serotype SAT 2 SAT2/ZIM/5/81 lineage (SAT2 ZIM 5-81 spreadsheet)
Supplementary Data 2 (SD2_pAb_info.xlsx file)
Characteristics of FMDV polyclonal antibody (pAb) **used for antigenic typing **- 198 archived bovine FMDV antisera (48 Serotype O, 70 Serotype A, 14 SAT 1, 31 SAT 2 and 35 multivalent sera shared between SAT 1 and SAT 2). The antisera had been raised against 40 vaccines with different vaccine strains and strain combinations.
Supplementary Data 3 (SD3_mAb_data.xlsx file)
Antigenic reactivity of a panel of 63 FMDV strains against serotype homologous mAbs - The assay used to analyse the reactivity of each virus with the serotype homologous Mabs was a trapping ELISA, performed as previously described (Samuel et al., 1991; Grazioli et al., 2013), but using recombinant integrin αvβ6 as universal capture ligand for FMDV in place of serotype-homologous polyclonal rabbit immune sera (Ferris et al., 2011; Shimmon et al., 2016). Values represent percentage of reactivity, normalised and expressed in a scale from 0 to 100.
Supplementary Data 4 (SD4_pAb_data.xlsx file)
Antigenic reactivity of a panel of 32 FMDV strains against serotype homologous pAbs - The virus neutralisation test (VNT) used was as described by Rweyemamu et al. (1984). Values represent log10 antibody titre.
Supplementary Data 5 (SD5_VNT_data.xlsx file)
Reciprocal VNT titres of cattle and guinea pigs after immunisation with different FMDV vaccine batches and tested against a panel of 16 FMDV strains - The test method followed the description in the WOAH Manual (Rweyemamu et al., 1984). Values represent antibody titre.
Reference
- Rweyemamu, M. M., Ouldridge, E. J., Head, M., & Purse, F. Evaluation of the antigenic variation within type-A foot and mouth disease virus isolates from Asia. J. Biol. Stand. 12, 191–194 (1984). https://doi.org/10.1016/s0092-1157(84)80053-0
- Grazioli, S., Fallacara, F., & Brocchi, E. Mapping of antigenic sites of foot-and-mouth disease virus serotype Asia 1 and relationships with sites described in other serotypes. J. Gen. Virol. 94, 559–569 (2013). https://doi.org/10.1099/vir.0.048249-0
- Samuel, A. R., Knowles, N. J., Samuel, G. D., & Crowther J. R. Evaluation of a trapping ELISA for the differentiation of foot-and-mouth disease virus using monoclonal antibodies. Biologicals 19, 299-310 (1991). https://doi.org/10.1016/s1045-1056(05)80019-3
- Ferris NP, Grazioli S, Hutchings GH, Brocchi E. (2011). Validation of a recombinant integrin αvβ6/monoclonal antibody based antigen ELISA for the diagnosis of foot-and-mouth disease. J Virol Methods. 175(2):253-60.
- Shimmon, G. et al. Truncated Bovine Integrin Alpha-v/Beta-6 as a Universal Capture Ligand for FMD Diagnosis. PLoS One 11, e0160696 (2016). https://doi.org/10.1371/journal.pone.0160696
Version Changes
20-May-2025 - Added additional information on SD3_mAb_data.xlsx and SD4_pAb_data.xlsx files to clarify data presented on antigenic reactivity of FMDV strains tested against serotype homologous mAbs and pAbs