Viola umphangensis (Violaceae), a new species from Thailand
Data files
Abstract
Fresh and dried materials were examined from field collections and herbarium specimens and measurements of vegetative and reproductive parts were taken using a dissecting microscope. Voucher specimens were prepared and deposited at BK, BKF and QBG (Table 1). Permanent cross-section slides of rhizomes, petioles and leaves were prepared using a paraffin method (Johansen 1940, Kermanee 2008). The permanent slides of the epidermis were prepared using a peeling method (Thammathaworn 1995). All permanent slides were observed under a light microscope (LM) (Zeiss-Axioskop 2) and stored at the Palynology Special Research Unit, Department of Botany, Faculty of Science, Kasetsart University, Bangkok, Thailand. The pollen grains were obtained using a modified acetolysis technique (Erdtman 1960). The pollen slides were investigated under a LM (Zeiss-primo star) and a scanning electron microscope (SEM) (FEI-Quanta 450) at the Scientific Equipment Center, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Dataset content overview
Figure 1. Distribution of Viola umphangensis S. Nansai, Srisanga & Suwanph. in Thailand, designed by a star.
Figure 2. Viola umphangensis S. Nansai, Srisanga & Suwanph. (A) habit, (B) stipules, (C) flower bud, (D) flower, (E) stamen, (F) stigma, (G) fruit, (H) seeds. Photos by S. Nansai (S. Nansai 10 (BK, BKF & QBG)).
Figure 3. Viola umphangensis S. Nansai, Srisanga & Suwanph. (A) habit, (B) leaf margin, (C–D) flower, (E) stamen, (F) pistil, (G) fruit, (H) seeds. Drawing by S. Nansai. (S. Nansai 10 (BK, BKF & QBG)).
Figure 4. Seed morphology from LM and SEM. (A–C) Viola umphangensis S. Nansai, Srisanga & Suwanph. (A) conspicuous raphe, (B) size, (C) areolate and smooth surface. All from S. Nansai 10 (BK, BKF & QBG). (D–F) V. betonicifolia Sm. (D) inconspicuous raphe, (E) size, (F) striate surface. All from S. Nansai 7 (BK, BKF & QBG).
Figure 5. Anatomical characters. (A–D) Viola umphangensis S. Nansai, Srisanga & Suwanph. (A) rhizome, (B) petiole, (C) upper epidermis, (D) lower epidermis. All from S. Nansai 20 (BK, BKF & QBG). (E–H) V. betonicifolia Sm. (E) rhizome, (F) petiole, (G) upper epidermis, (H) lower epidermis. All from S. Nansai 32 (BK, BKF & QBG). Abbreviation: cb = cortical bundle.
Figure 6. Pollen morphology from LM. (A–C) Viola umphangensis S. Nansai, Srisanga & Suwanph. (A) polar view, (B) equatorial view, (C) colporate. All from S. Nansai 10 (BK, BKF & QBG). (D–F) V. betonicifolia Sm. (D) polar view, (E) equatorial view, (F) colporate. All from S. Nansai 7 (BK, BKF & QBG).
Figure 7. Pollen morphology from SEM. (A–C) Viola umphangensis S. Nansai, Srisanga & Suwanph. (A) polar view, (B) equatorial view, (C) granulate. All from S. Nansai 10 (BK, BKF & QBG). (D–F) V. betonicifolia Sm. (D) polar view, (E) equatorial view, (F) granulate fossula. All from S. Nansai 7 (BK, BKF & QBG).