Data from: Miniaturized optically-generated Bessel beam ultrasound for volumetric transcranial brain stimulation
Data files
Mar 20, 2026 version files 394.09 KB
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Figure_3E.xlsx
9.47 KB
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Figure1G.xlsx
10.90 KB
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Figure2B.xlsx
41.18 KB
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Figure5D.xlsx
11.82 KB
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README.md
3.28 KB
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Ultrasound_2.8_MPa.zip
105.77 KB
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Ultrasound_3.7_MPa.zip
105.84 KB
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Ultrasound_4.1_MPa.zip
105.84 KB
Abstract
Non-invasive stimulation of small, variably shaped brain sub-regions is crucial for advancing our understanding of brain functions. Current ultrasound neuromodulation faces two significant trade-offs when targeting brain sub-regions: miniaturization versus volumetric control, and spatial resolution versus transcranial capability. Here, we present an optically-generated Bessel beam ultrasound (OBUS) device designed to overcome these limitations. This miniaturized device, measuring 2.33 mm in diameter, delivers a column-shaped field achieving a lateral resolution of 152 μm and an axial resolution of 1.93 mm, enabling targeting of brain sub-regions with an elongated volume of tissue activation. Immunofluorescence imaging of mouse brain slices confirms its ability to stimulate cells at depths up to 2.1 mm. Additionally, OBUS outperforms conventional Gaussian ultrasound in transcranial transmission efficiency and beam shape preservation. Electrophysiological recordings and functional MRI captured rodent brain responses evoked by OBUS, demonstrating OBUS’s ability to non-invasively activate neural circuits in intact brains. This technology offers new possibilities for studying brain functions with precision and volumetric control.
Dataset DOI: 10.5061/dryad.zgmsbccr0
Description of the data and file structure
- Figure 1G includes the peak amplitude data of the acoustic signals acquired.
- Figure 2B includes the axial profile of the simulated acoustic field.
- Figure 3E.xlsx includes all the persons' coefficients acquired from ImageJ.
- Ultrasound_2.8_MPa.zip, Ultrasound_3.7_MPa, and Ultrasound_4.1_MPa include all the raw electrophysiology data acquired in three mice.
- Figure 5D includes the average and standard deviation of the BOLD signal for subjects 1 and 2.
Files and variables
File: Figure1G.xlsx
Description: Peak-to-peak value of the acoustic signals acquired.
Variables
- PDMS ratio, unit: NA.
- Amplitude, unit: mV.
File: Figure2B.xlsx
Description: The data files contain measurements of ultrasound field characteristics across different device types and skull conditions.
Column descriptions:
| Column name | Description | Unit |
|---|---|---|
| OBUS no skull Distance | Axial distance from the OBUS device measured in the absence of skull | mm |
| OBUS no skull Intensity | Ultrasound intensity from the OBUS device measured in the absence of skull | arbitrary unit |
| OBUS with skull Distance | Axial distance from the OBUS device measured through rat skull | mm |
| OBUS with skull Intensity | Ultrasound intensity from the OBUS device measured through rat skull | arbitrary unit |
| SOAP no skull Distance | Axial distance from the SOAP device measured in the absence of skull | mm |
| SOAP no skull Intensity | Ultrasound intensity from the SOAP device measured in the absence of skull | arbitrary unit |
| SOAP with skull Distance | Axial distance from the SOAP device measured through rat skull | mm |
| SOAP with skull Intensity | Ultrasound intensity from the SOAP device measured through rat skull | arbitrary unit |
File: Figure_3E.xlsx
Description: Pearson's coefficient calculated by ImageJ
Variables
- Depth of the brain slices, unit: mm.
- Pearson's coefficient, unit: NA.
File: Ultrasound_3.7_MPa.zip
Description: Power spectral density of the electrophysiological recording of the three mice.
Variables
- Frequency: Hz.
- Amplitude: dB/Hz.
File: Ultrasound_2.8_MPa.zip
Description: Power spectral density of the electrophysiological recording of the three mice.
Variables
- Frequency: Hz.
- Amplitude: dB/Hz.
File: Ultrasound_4.1_MPa.zip
Description: Power spectral density of the electrophysiological recording of the three mice.
Variables
- Frequency, unit: Hz.
- Amplitude, unit: dB/Hz.
File: Figure5D.xlsx
Description: The average and standard deviation of the BOLD signal for subjects 1 and 2
Variables
- Time, unit: second.
- Amplitude, unit: arbitrary units.