Child dataset 1 of 4 from: Single and half-cell RNA-sequencing in Stentor coeruleus control, beta-tubulin, and dynein knockdown cells
Data files
May 08, 2026 version files 146.89 GB
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A1_S1_L001_R1_001.fastq.gz
6.91 GB
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A1_S1_L001_R2_001.fastq.gz
7.32 GB
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A2_S2_L001_R1_001.fastq.gz
8.12 GB
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A2_S2_L001_R2_001.fastq.gz
8.54 GB
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A5_S5_L001_R1_001.fastq.gz
7.69 GB
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A5_S5_L001_R2_001.fastq.gz
8.09 GB
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A6_S6_L001_R1_001.fastq.gz
3.06 GB
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A6_S6_L001_R2_001.fastq.gz
3.22 GB
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metadata.csv
7.93 KB
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P1_S3_L001_R1_001.fastq.gz
7.95 GB
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P1_S3_L001_R2_001.fastq.gz
8.39 GB
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P2_S4_L001_R1_001.fastq.gz
8.69 GB
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P2_S4_L001_R2_001.fastq.gz
9.16 GB
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P5_S7_L001_R1_001.fastq.gz
23.75 GB
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P5_S7_L001_R2_001.fastq.gz
25.33 GB
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P6_S8_L001_R1_001.fastq.gz
5.20 GB
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P6_S8_L001_R2_001.fastq.gz
5.47 GB
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README.md
1.32 KB
Abstract
Stentor coeruleus is a giant single-celled ciliates that reach lengths of up to 1 mm. Because of their large size, these cells are easy to manipulate, and they also possess the ability to heal wounds and regenerate. These properties are ideal for investigating subcellular pattern formation, as we can physically dissect the cells into anterior and posterior halves. We used bulk RNA-sequencing to assay for regionalized mRNAs in the anterior or posterior half of the cell.
Dataset DOI: 10.5061/dryad.zpc866tjd
Description of the data and file structure
Additional Dataset 1 of 4. See parent entry for more: https://doi.org/10.6078/D1WT6W
This dataset contains paired-end RNA-sequencing data in anterior (A) versus posterior (P) bisected Stentor coeruleus. The first character in the file names correspond to anterior or posterior (A or P) and the second character corresponds to the biological replicate number.
metadata.csv contains file name, which experiment the file is from (bulk, tubulin, or dynein), condition (control or knockdown), region (anterior or posterior), and biological replicate.
Files:
A1_S1_L001_R1_001.fastq.gz
A1_S1_L001_R2_001.fastq.gz
A2_S2_L001_R1_001.fastq.gz
A2_S2_L001_R2_001.fastq.gz
A5_S5_L001_R1_001.fastq.gz
A5_S5_L001_R2_001.fastq.gz
A6_S6_L001_R1_001.fastq.gz
A6_S6_L001_R2_001.fastq.gz
P1_S3_L001_R1_001.fastq.gz
P1_S3_L001_R2_001.fastq.gz
P2_S4_L001_R1_001.fastq.gz
P2_S4_L001_R2_001.fastq.gz
P5_S7_L001_R1_001.fastq.gz
P5_S7_L001_R2_001.fastq.gz
P6_S8_L001_R1_001.fastq.gz
P6_S8_L001_R2_001.fastq.gz
Within 20 minutes of bisection, 50 anteriors and 50 posteriors were placed in Trizol with as little water leftover as possible. RNA was extracted using a Direct-zol RNA Purification Kit (Zymo), and libraries were prepped using the NEBNext Ultra II RNA Library Prep Kit (NEB) following manufacturer’s instructions. Samples were pooled and submitted for sequencing on an Illumina NovaSeqX (SP300).
See parent entry for more: https://doi.org/10.6078/D1WT6W