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Programmable liposome organization via DNA origami templates

Zhang, Zhao12; Feng, Zhaomeng2; Zhao, Xiaowei1; Yu, Zhiheng1; Chapman, Edwin12

Published Sep 05, 2025 on Dryad. https://doi.org/10.5061/dryad.k0p2ngfm1

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Sep 05, 2025 version files 2.19 GB

Abstract

Liposomes are essential vehicles for membrane protein reconstitution and drug delivery, making them vital tools in both in vivo and in vitro studies. However, the lack of robust techniques for the precise arrangement of these synthetic vesicles limits their potential applications. Here, we present a modular polymerization platform based on square DNA origami to template the formation and organization of liposomes. By programming the sequence, number, position, chirality, and flexibility of sticky ends on each square, we assemble uniformly sized liposomes into diverse two-dimensional (2D) arrays, as well as finite lattices and rings. Additionally, we demonstrate stepwise assembly and targeted disassembly, enabling dynamic structural control. These complex liposome architectures represent a significant advancement in the fields of biotechnology, nanotechnology, and bottom-up biology.