GAS5 protects against osteoporosis by targeting UPF1/SMAD7 axis in osteoblast differentiation
Data files
Apr 23, 2024 version files 688.99 MB
-
Figure_1.rar
185.41 MB
-
Figure_2.rar
144.75 MB
-
Figure_3.rar
104.17 MB
-
Figure_4.rar
9.78 MB
-
Figure_5.rar
70.41 MB
-
Figure_6.rar
92.57 MB
-
Figure_7.rar
72.34 MB
-
README.md
2.39 KB
-
Supplementary_Figure.rar
9.57 MB
Abstract
Osteoporosis is a common systemic skeletal disorder resulting in bone fragility and increased fracture risk. It is still necessary to explore its detailed mechanisms and identify novel targets for the treatment of osteoporosis. Previously, we found that an lncRNA named GAS5 in humans could negatively regulate lipoblast/adipocyte differentiation. However, it is still unclear whether GAS5 affects osteoblast differentiation and whether GAS5 is associated with osteoporosis. Our current research found that GAS5 was decreased in the bones and BMSCs, a major origin of osteoblast, in osteoporosis patients. Mechanistically, GAS5 promotes osteoblast differentiation by interacting with UPF1 to degrade Smad7 mRNA. Moreover, a decreased bone mass and impaired bone repair ability were observed in Gas5 heterozygous mice, manifesting in osteoporosis. The systemic supplement of Gas5-overexpressing adenoviruses significantly ameliorated bone loss in an osteoporosis mouse model. In conclusion, GAS5 promotes osteoblast differentiation by targeting the UPF1/Smad7 axis and protects against osteoporosis.
https://doi.org/10.5061/dryad.9cnp5hqfj
By studying the GAS5 gene in MSCs cells and animals, we found that GAS5 can promote MSCs osteoblast differentiation and protect against osteoporosis by targeting UPF1/SMAD7. This dataset includes the study data and original images of the research.
Description of the data and file structure
This dataset is divided according to each Figure. The raw data for each Figure corresponds separately to the folder in it. Images of the supplementary data included in the study are also presented in this dataset.
Staining Data
- ALP: alkaline phosphatase staining
- ARS: alizarin red staining
- HE: hematoxylin-eosin staining
- MASSON: Masson staining
- COL1: COL1A1 immunohistochemical staining
- OCN: BGLAP or Bglap immunohistochemical staining
Statistic Data
The statistical data of each experiment have been put in Excel tables. The information represented by the variables is as follows:
Figure 1:
- Normal: Normal population
- Dysplasia: Patient with developmental dysplasia of the hip
- Osteoporosis: Patient with osteoporosis
- Absorbance: The absorbance of alizarin red stain after extraction
- Cyto: cytoplasm
- Nu: nucleus
Figure 2: shCtrl/shGAS5-1/shGAS5-2 represent controls, GAS5 knocks down viruses 1 and 2, respectively.
Figure 3: Vector/OE-GAS5 represents an empty vector, and GAS5 overexpresses lentiviruses, respectively.
Figure 4: Flag-FL/Flag-CH/Flag-HD/Flag-CH-HD/Flag-HD-SQ represent different segment structure of UPF1.
Figure 5: si stands for knocked down siRNA of different molecules, and OE stands for overexpression of different molecules.
Figure 6: GAS5+/+ represents the wild mouse, and GAS5+/- stands for knocking out heterozygotes.
Figure 7: NS. + Vector means the group of normal saline and empty vector, DXMS + Vector means the group of dexamethasone and empty vector, DXMS+GAS5-OE means the group of dexamethasone and GAS5 overexpression adenoviruses.
The variables in the supplementary figure are consistent with those described above.
Sharing/Access information
There are no other externally accessible datasets for this dataset.
Code/Software
No special code or software is involved in this dataset.
This dataset was obtained through various experiments, and statistical graphs were obtained after statistical analysis by SPSS software.