Here we present a statistically rigorous approach to quantifying microarray expression data that allows the relative effects of multiple classes of treatment to be compared and incorporates analytical methods that are common to quantitative genetics. From the magnitude of gene effects and contributions of variance components, we find that gene expression in adult flies is affected most strongly by sex, less so by genotype and only weakly by age (for 1- and 6-wk flies); in addition, sex x genotype interactions may be present for as much as 10% of the Drosophila transcriptome. This interpretation is compromised to some extent by statistical issues relating to power and experimental design. Nevertheless, we show that changes in expression as small as 1.2-fold can be highly significant. Genotypic contributions to transcriptional variance may be of a similar magnitude to those relating to some quantitative phenotypes and should be considered when assessing the significance of experimental treatments.
MFCS15a2 Data Set
Original ScanAlyze file
MFCS15a2.DAT
RR Slide 11b_Cy3
RRSlide11b.Cy3.TIF
MFCS3b Data Set
Original ScanAlyze file
MFCS3b.DAT
MFCS1a Data Set
Original ScanAlyze file
MFCS1a.DAT
RR Slide 16b_Cy3
RRSlide16b.Cy3.Tif
READ ME for Aging
Original ScanAlyze file
READ ME for Aging.doc
MFCS6a Data Set
Original ScanAlyze file
MFCS6a.DAT
MFCS5a Data Set
Original ScanAlyze file
MFCS5a.DAT
MFCS9a Data Set
Original ScanAlyze file
MFCS9a.DAT
MFCS7a Data Set
Original ScanAlyze file
MFCS7a.DAT
MFCS4a Data Set
Original ScanAlyze file
MFCS4a.DAT
MFCS11a Data Set
Original ScanAlyze file
MFCS11a.DAT
MFCS4b Data Set
Original ScanAlyze files
MFCS4b.DAT
UMFOS data set
Gene Expression Profiling Results; this is an excel file with all of the extracted raw fluorescence intensities for Jin et al at the top, log2 at the bottom
UMFOS.xls
RR Slide 10b_Cy5
RRSlide10b.Cy5.TIF
SGA sig Data Set
Gene Expression Profiling Results
SGAsig.xls
MFCS10b Data Set
Original ScanAlyze file
MFCS10b.DAT
MFCS15b2 Data Set
Original ScanAlyze file
MFCS15b2.DAT
RR Slide 2a Data Set
Original ScanAlyze file
RRslide2a.DAT
MFCS11b Data Set
Original ScanAlyze file
MFCS11b.DAT
MFCS10a Data Set
Original ScanAlyze file
MFCS10a.DAT
MFCS16a2 Data Set
Original ScanAlyze file
MFCS16a2.DAT
RR Slide 16b_Cy5
RRSlide16b.Cy5.Tif
MFCS9b Data Set
Original ScanAlyze file
MFCS9b.DAT
RR Slide 16a_Cy3
RRSlide16a.Cy3.Tif
MFCS5b Data Set
Original ScanAlyze file
MFCS5b.DAT
RR Slide 10b_Cy3
RRSlide10b.Cy3.TIF
RR Slide 11b_Cy5
RRSlide11b.Cy5.TIF
MFCS12a2 Data Set
Original ScanAlyze file
MFCS12a2.DAT
MFCS3a Data Set
Original ScanAlyze file
MFCS3a.DAT
Template Data Set
This is a pdf showing the location of the 48 12x8 grids used to collect the data. Note that there are only 8 clones in the last grid of each sector [12, 24, 36, and 28]
Template.pdf
Sex Specific List Data Set
Gene Expression Profiling Results
SexSpecificList.txt
RR Slide 16a_Cy5
RRSlide16a.Cy5.Tif
RR Slide 2b Data Set
Original ScanAlyze file
RRslide2b.DAT
MFCS7b Data Set
Original ScanAlyze file
MFCS7b.DAT
UMFOS text file
Gene Expression Profiling Results; this file contains the normalized relative fluorescence intensities used in the gene-specific models.
UMFOS.txt
MFCS6b Data Set
Original ScanAlyze file
MFCS6b.DAT
MFCS1b Data Set
Original ScanAlyze file
MFCS1b.DAT
Clone LUT Data Set
Gene Expression Profiling Results; an Excel file listing the clones, their identity, and whether the sequence was reconfirmed after arraying
CloneLUT.xls