Transcriptional regulatory networks play a central role in optimizing cell survival. How DNA binding domains and cis-regulatory DNA binding sequences have co-evolved to allow the expansion of transcriptional networks and how this contributes to cellular fitness remains unclear. Here we experimentally explore how the complex G1/S transcriptional network evolved in the budding yeast Saccharomyces cerevisiae by examining different chimeric transcription factor (TF) complexes. Over 300 G1/S genes are regulated by either one of the two TF complexes, SBF and MBF, which bind to specific DNA binding sequences, SCB and MCB, respectively. Our data suggests that whilst SBF is the likely ancestral regulatory complex, the ancestral DNA binding element is more MCB-like. G1/S network expansion took place by both cis- and trans- co-evolutionary changes in closely related but distinct regulatory sequences. Replacement of the endogenous SBF DNA-binding domain (DBD) with that from more distantly related fungi leads to a contraction of the G1/S network in budding yeast, which also correlates with increased defects in cell growth, cell size, and proliferation. This indicates that expansion of the G1/S network in budding yeast may represent an evolutionary product of selection for cell cycle fitness.
Calb_mbp1_C6_0
The raw data results of RNA-seq for the sample Calb_mbp1_C6_0
Calb_mbp1_C6_30
The data results of the RNA-seq for the sample Calb_mbp1_C6_30
Delta_swi4_C6_0
The data results of the RNA-seq for the sample Delta_swi4_C6_0
Delta_swi4_C6_30
The data results for RNA-seq for the sample Delta_swi4_C6_30
Klac_mbp1_C6_0
The RNA-seq data results for the sample Klac_mbp1_C6_0
Klac_mbp1_C6_30
The RNA-seq data results for the sample Klac_mbp1_C6_30
Ncra_BD_C6_0
The RNA-seq data results for the sample Ncra_BD_C6_0
Ncra_BD_C6_30
The RNA-seq data results for the sample Ncra_BD_C6_30
RES2_BD_C6_0
The RNA-seq data results for the sample RES2_BD_C6_0
RES2_BD_C6_30
The RNA-seq data results for the sample RES2_BD_C6_30
WT_C6_0
The RNA seq data results for the sample WT_C6_0
WT_C6_30
The RNA seq data results for the sample WT_C6_30
Calb_mbp1_C7_0
The RNA-seq data results for the sample Calb_mbp1_C7_0
Calb_mbp1_C7_30
The RNA-seq data results for the sample Calb_mbp1_C7_30
Delta_swi4_C7_0
The RNA-seq data results for the sample Delta_swi4_C7_0
Delta_swi4_C7_30
The RNA-seq data results for the sample Delta_swi4_C7_30
Klac_mbp1_C7_0
The RNA-seq data results for the sample Klac_mbp1_C7_0
Klac_mbp1_C7_30
The RNA-seq data results for the sample Klac_mbp1_C7_30
Ncra_BD_C7_0
The RNA-seq data results for the sample Ncra_BD_C7_0
Ncra_BD_C7_30
The RNA-seq data results for the sample Ncra_BD_C7_30
RES2_BD_C7_0
The RNA-seq data results for the sample RES2_BD_C7_0
RES2_BD_C7_30
The RNA-seq data results for the sample RES2_BD_C7_30
WT_C7_0
The RNA-seq data results for the sample WT_C7_0
WT_C7_30
The RNA-seq data results for the sample WT_C7_30
Calb_mbp1_C8_0
The RNA-seq data results for the sample Calb_mbp1_C8_0
Calb_mbp1_C8_30
The RNA-seq data results for the sample Calb_mbp1_C8_30
Delta_swi4_C8_0
The RNA-seq data results for the sample Delta_swi4_C8_0
Delta_swi4_C8_30_
The RNA-seq data results for the sample Delta_swi4_C8_30
Klac_mbp1_C8_0
The RNA-seq data results for the sample Klac_mbp1_C8_0
Klac_mbp1_C8_30
The RNA-seq data results for the sample Klac_mbp1_C8_30
Ncra_BD_C8_0
The RNA-seq data results for the sample Ncra_BD_C8_0
Ncra_BD_C8_30
The RNA-seq data results for the sample Ncra_BD_C8_30
RES2_BD_C8_0
The RNA-seq data results of the sample RES2_BD_C8_0
RES2_BD_C8_30
The RNA-seq data results for the sample RES2_BD_C8_30
WT_C8_0
The RNA-seq data results for the sample WT_C8_0
WT_C8_30
The RNA-seq data results of the sample WT_C8_30