Data from: Plastic particles adsorb to the roots of freshwater vascular plant Spirodela polyrhiza but do not impair growth

Dovidat L, Brinkmann B, Vijver M, Bosker T

Date Published: August 14, 2019

DOI: https://doi.org/10.5061/dryad.53pp6tt

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Title chlorophyll measurements
Downloaded 1 time
Description Determination of photosynthetic activity by duckweed (Spirodela polyrhiza) exposed to 50nm and 500nm plastic particles in concentrations of 102, 104 and 106 particles mL-1 for 120 hours. The photosynthetic activity, which was measured through chlorophyll contents, of the exposed duckweed was compared to non-exposed control organisms, with 3 replicates per treatment. The experiment measured the endpoints of chlorophyll a, chlorophyll b and total chlorophyll contents after 120 hours, employing spectrophotometrical determination of absorbance.
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Title data_dictionary
Downloaded 2 times
Description Data dictionary for all endpoints
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Title freshweight
Downloaded 1 time
Description Growth inhibition study that experimentally exposed duckweed (Spirodela polyrhiza) to 50nm and 500nm plastic particles in concentrations ranging from 102 to 106 particles mL-1 for 120 hours and compared these to non-exposed control organisms, with 8 replicates per exposure treatment. The fresh weight was determined 0 hours and 120 hours of exposure to plastic particles by carefully patting the individual plants dry and subsequently transferring them to an analytical balance with a precision of four decimals.
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Title frond
Downloaded 1 time
Description Growth inhibition study that experimentally exposed duckweed (Spirodela polyrhiza) to 50nm and 500nm plastic particles in concentrations ranging from 102 to 106 particles mL-1 for 120 hours and compared these to non-exposed control organisms, with 8 replicates per exposure treatment. The frond area was recorded at the beginning (0 hours of exposure) and a the end of the experiment (120 hours of exposure) by taking a vertical photograph of the test wells, which were placed onto millimeter paper for scale purposes, with a Nikon D3100 digital single lens reflex camera (Nikon, Miniato, Tokyo, Japan) and a 18-55mm lens (Nikon, Miniato, Tokyo, Japan). The frond area was determined to a precision of four digits on the acquired images using the image analysis software Fiji (Schindelin et al., 2012). Frond numbers were counted manually on the acquired images.
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Title roots
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Description Growth inhibition study that experimentally exposed duckweed (Spirodela polyrhiza) to 50nm and 500nm plastic particles in concentrations ranging from 102 to 106 particles mL-1 for 120 hours and compared these to non-exposed control organisms, with 8 replicates per exposure treatment. The root length was recorded at 0 hours and 120 hours of exposure by transferring single plants onto millimeter paper and taking a vertical photograph using a USB microscope (Dino-Lite USB, AnMo Electronics Corporation, New Taipei City, Taiwan). The root length was measured with a precision of four digits on the acquired images employing Fiji (Schindelin et al., 2012), and the root number was determined manually.
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Title Dovidat et al Metadata
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Description Metadata file
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Citation is not yet available for this publication from Limnology and Oceanography Letters. It will become available shortly after the publication appears.

Additionally, please cite the Dryad data package:

Dovidat L, Brinkmann B, Vijver M, Bosker T (2019) Data from: Plastic particles adsorb to the roots of freshwater vascular plant Spirodela polyrhiza but do not impair growth. Dryad Digital Repository. https://doi.org/10.5061/dryad.53pp6tt
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