Data from: Reciprocal changes of H3K27ac and H3K27me3 at the promoter regions of the critical genes for endometrial decidualization

Katoh, Noriko, Juntendo University

Kuroda, Keiji, Juntendo University

Tomikawa, Junko, Juntendo University

Ogata-Kawata, Hiroko, Juntendo University

Ozaki, Rie, Juntendo University

Ochiai, Asako, Juntendo University

Kitade, Mari, Juntendo University

Takeda, Satoru, Juntendo University

Nakabayashi, Kazuhiko, Department of Maternal-Fetal Biology, National Research Institute for Child Health & Development, Tokyo 157-8535, Japan

Hata, Kenichiro, Department of Maternal-Fetal Biology, National Research Institute for Child Health & Development, Tokyo 157-8535, Japan

Published Aug 23, 2019 on Dryad. https://doi.org/10.5061/dryad.97s68c2

Cite this dataset

Katoh, Noriko et al. (2019). Data from: Reciprocal changes of H3K27ac and H3K27me3 at the promoter regions of the critical genes for endometrial decidualization [Dataset]. Dryad. https://doi.org/10.5061/dryad.97s68c2

Abstract

Aim: Decidualization is essential for embryo implantation and placental development. We aimed to obtain transcriptome and epigenome profiles for primary endometrial stromal cells (ESCs) and in vitro decidualized cells. Materials & methods: ESCs isolated from human endometrial tissues remained untreated (D0), or decidualized for 4 days (D4) and 8 days (D8) in the presence of 8-bromo-cAMP and progesterone. Results: Among the epigenetic modifications examined (DNA methylation, H3K27ac, H3K9me3 and H3K27me3), the H3K27ac patterns changed most dramatically, with a moderate correlation with gene expression changes, upon decidualization. Subsets of up- and down-regulated genes upon decidualization were associated with reciprocal changes of H3K27ac and H3K27me3 modifications at their promoter region, and were enriched with genes essential for decidualization such as WNT4, ZBTB16, PROK1 and GREB1. Conclusion: Our dataset is useful to further elucidate the molecular mechanisms underlying decidualization.

Usage notes

Tables for fpkm values

Tables for fpkm values for RNA-seq data calculated using Cufflinks:genes.fpkm_tracking, isoforms.fpkm_tracking, and tss_groups.fpkm_tracking

fpkm_tracking.zip

Peak call files for ChIP-seq data

Peak call files for CHIP-seq data produced by MACS2:EM0409D0_K27ac_macs2_peaks.narrowPeak EM0409D0_K27me3_macs2_peaks.broadPeak EM0409D0_K9me3_macs2_peaks.broadPeak EM0409D4_K27ac_macs2_peaks.narrowPeak EM0409D4_K27me3_macs2_peaks.broadPeak EM0409D4_K9me3_macs2_peaks.broadPeak EM0409D8_K27ac_macs2_peaks.narrowPeak EM0409D8_K27me3_macs2_peaks.broadPeak EM0409D8_K9me3_macs2_peaks.broadPeak EM0519D0_K27ac_macs2_peaks.narrowPeak EM0519D0_K27me3_macs2_peaks.broadPeak EM0519D0_K9me3_macs2_peaks.broadPeak EM0519D4_K27ac_macs2_peaks.narrowPeak EM0519D4_K27me3_macs2_peaks.broadPeak EM0519D4_K9me3_macs2_peaks.broadPeak EM0519D8_K27ac_macs2_peaks.narrowPeak EM0519D8_K27me3_macs2_peaks.broadPeak EM0519D8_K9me3_macs2_peaks.broadPeak

macs2.peakcalls.zip

bigwig_files

"bigwig_files.zip" (md5 checksum: a7cee3f55c6c3d53dd562475aa7fc1f3). The "bigwig_files.zip" file contains the following 25 bigwig files (19 files for ChIP-seq data and 6 files for RNA-sea data). EM0409_D0_H3K27ac.hg19.bw EM0409_D0_H3K27me3.hg19.bw EM0409_D0_H3K9me3.hg19.bw EM0409_D4_H3K27ac.hg19.bw EM0409_D4_H3K27me3.hg19.bw EM0409_D4_H3K9me3.hg19.bw EM0409_D4_input.hg19.bw EM0409_D8_H3K27ac.hg19.bw EM0409_D8_H3K27me3.hg19.bw EM0409_D8_H3K9me3.hg19.bw EM0519_D0_H3K27ac.hg19.bw EM0519_D0_H3K27me3.hg19.bw EM0519_D0_H3K9me3.hg19.bw EM0519_D4_H3K27ac.hg19.bw EM0519_D4_H3K27me3.hg19.bw EM0519_D4_H3K9me3.hg19.bw EM0519_D4_input.hg19.bw EM0519_D8_H3K27me3.hg19.bw EM0519_D8_H3K9me3.hg19.bw RNAseq_EM0409_D0.hg19.bw RNAseq_EM0409_D4.hg19.bw RNAseq_EM0409_D8.hg19.bw RNAseq_EM0519_D0.hg19.bw RNAseq_EM0519_D4.hg19.bw RNAseq_EM0519_D8.hg19.bw