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dc.contributor.author El Meouche, Imane
dc.contributor.author Dunlop, Mary J.
dc.date.accessioned 2018-11-08T19:51:28Z
dc.date.available 2018-11-08T19:51:28Z
dc.date.issued 2018-11-08
dc.identifier doi:10.5061/dryad.n1h9d0d
dc.identifier.citation El Meouche I, Dunlop MJ (2018) Heterogeneity in efflux pump expression predisposes antibiotic-resistant cells to mutation. Science 362(6415): 686-690.
dc.identifier.uri http://hdl.handle.net/10255/dryad.192784
dc.description Antibiotic resistance is often the result of mutations that block drug activity; however, bacteria also evade antibiotics by transiently expressing genes such as multidrug efflux pumps. A crucial question is whether transient resistance can promote permanent genetic changes. Previous studies have established that antibiotic treatment can select tolerant cells that then mutate to achieve permanent resistance. Whether these mutations result from antibiotic stress or preexist within the population is unclear. To address this question, we focused on the multidrug pump AcrAB-TolC. Using time-lapse microscopy, we found that cells with higher acrAB expression have lower expression of the DNA mismatch repair gene mutS, lower growth rates, and higher mutation frequencies. Thus, transient antibiotic resistance from elevated acrAB expression can promote spontaneous mutations within single cells.
dc.relation.haspart doi:10.5061/dryad.n1h9d0d/1
dc.relation.isreferencedby doi:10.1126/science.aar7981
dc.title Data from: Heterogeneity in efflux pump expression predisposes antibiotic-resistant cells to mutation
dc.type Article
dc.contributor.correspondingAuthor Dunlop, Mary J.
prism.publicationName Science

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