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dc.contributor.author Kuntz, Steven Gregory
dc.contributor.author Eisen, Michael B.
dc.date.accessioned 2014-07-11T14:13:59Z
dc.date.available 2014-07-11T14:13:59Z
dc.date.issued 2014-04-24
dc.identifier doi:10.5061/dryad.s0p50
dc.identifier.citation Kuntz SG, Eisen MB (2014) Drosophila embryogenesis scales uniformly across temperature in developmentally diverse species. PLoS Genetics 10(4): e1004293.
dc.identifier.uri http://hdl.handle.net/10255/dryad.60698
dc.description Temperature affects both the timing and outcome of animal development, but the detailed effects of temperature on the progress of early development have been poorly characterized. To determine the impact of temperature on the order and timing of events during Drosophila melanogaster embryogenesis, we used time-lapse imaging to track the progress of embryos from shortly after egg laying through hatching at seven precisely maintained temperatures between 17.5°C and 32.5°C. We employed a combination of automated and manual annotation to determine when 36 milestones occurred in each embryo. D. melanogaster embryogenesis takes 33 hours at 17.5°C, and accelerates with increasing temperature to a low of 16 hours at 27.5°C, above which embryogenesis slows slightly. Remarkably, while the total time of embryogenesis varies over two fold, the relative timing of events from cellularization through hatching is constant across temperatures. To further explore the relationship between temperature and embryogenesis, we expanded our analysis to cover ten additional Drosophila species of varying climatic origins. Six of these species, like D. melanogaster, are of tropical origin, and embryogenesis time at different temperatures was similar for them all. D. mojavensis, a sub-tropical fly, develops slower than the tropical species at lower temperatures, while D. virilis, a temperate fly, exhibits slower development at all temperatures. The alpine sister species D. persimilis and D. pseudoobscura develop as rapidly as tropical flies at cooler temperatures, but exhibit diminished acceleration above 22.5°C and have drastically slowed development by 30°C. Despite ranging from 13 hours for D. erecta at 30°C to 46 hours for D. virilis at 17.5°C, the relative timing of events from cellularization through hatching is constant across all species and temperatures examined here, suggesting the existence of a previously unrecognized timer controlling the progress of embryogenesis that has been tuned by natural selection as each species diverges.
dc.relation.haspart doi:10.5061/dryad.s0p50/1
dc.relation.haspart doi:10.5061/dryad.s0p50/2
dc.relation.haspart doi:10.5061/dryad.s0p50/3
dc.relation.haspart doi:10.5061/dryad.s0p50/4
dc.relation.haspart doi:10.5061/dryad.s0p50/5
dc.relation.haspart doi:10.5061/dryad.s0p50/6
dc.relation.haspart doi:10.5061/dryad.s0p50/7
dc.relation.haspart doi:10.5061/dryad.s0p50/8
dc.relation.haspart doi:10.5061/dryad.s0p50/9
dc.relation.haspart doi:10.5061/dryad.s0p50/10
dc.relation.isreferencedby doi:10.1371/journal.pgen.1004293
dc.relation.isreferencedby PMID:24762628
dc.subject developmental clock
dc.subject developmental morphology
dc.subject development
dc.subject Embryogenesis
dc.subject Temperature
dc.subject developmental timing
dc.subject embryonic clock
dc.subject time-lapse imaging
dc.subject Q10
dc.subject growth rate
dc.subject cross-species comparison
dc.title Data from: Drosophila embryogenesis scales uniformly across temperature in developmentally diverse species
dc.type Article
dwc.ScientificName Drosophila ananassae
dwc.ScientificName Drosophila persimilis
dwc.ScientificName Drosophila pseudoobscura
dwc.ScientificName Drosophila willistoni
dwc.ScientificName Drosophila sechellia
dwc.ScientificName Drosophila simulans
dwc.ScientificName Drosophila mojavensis
dwc.ScientificName Drosophila virilis
dwc.ScientificName Drosophila erecta
dwc.ScientificName Drosophila yakuba
dwc.ScientificName Drosophila melanogaster
prism.publicationName PLoS Genetics
dryad.dansTransferDate 2018-04-18T14:31:23.936+0000
dryad.dansEditIRI https://easy.dans.knaw.nl/sword2/container/4fef92ee-0e4e-41de-a99f-0679f80548bd
dryad.dansArchiveDate 2018-04-23T12:57:13.382+0000

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Title D. melanogaster with labelled stages
Downloaded 16 times
Description D. melanogaster development with morphological stages listed. Scaling is 15 minutes of development per second of video.
Download Label_Me_allTemps_75.avi (48.67 Mb)
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Title Drosophila melanogaster, slow version of embryonic developmental stages
Downloaded 15 times
Description Time-lapse of D. melanogaster embryo developing with major stages listed. The pace of this video is reduced to facilitate the observation of each developmental stage.
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Title D. melanogaster at 7 temperatures
Downloaded 7 times
Description D. melanogaster development at 7 different temperatures (17.5˚C, 20˚C, 22.5˚C, 25˚C, 27.5˚C, 30˚C, and 32.5˚C). Scaling is 15 minutes of development per second of video.
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Title 11 species at 17.5C
Downloaded 18 times
Description Development of eleven species of Drosophila at 17.5˚C. Every second of video is 15 minutes of development.
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Title 11 species at 22.5C
Downloaded 9 times
Description Seven species (D. ananassae, D. erecta, D. melanogaster, D. mojavensis, D. sechellia, D. simulans, D. virilis, D. willistoni, and D. yakuba) developing at 22.5˚C. Every second of video is 15 minutes of development.
Download 22.5_allTemps_5.avi (253.6 Mb)
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Title 11 species at 27.5C
Downloaded 6 times
Description Seven species (D. ananassae, D. erecta, D. melanogaster, D. mojavensis, D. sechellia, D. simulans, D. virilis, D. willistoni, and D. yakuba) developing at 27.5˚C. Every second of video is 15 minutes of development.
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Title D. ananassae at 7 temperatures
Downloaded 8 times
Description D. ananassae development at 7 different temperatures (17.5˚C, 20˚C, 22.5˚C, 25˚C, 27.5˚C, 30˚C, and 32.5˚C). Scaling is 15 minutes of development per second of video.
Download Dana_allTemps_25.avi (286.2 Mb)
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Title D. mojavensis at 6 temperatures
Downloaded 7 times
Description D. mojavensis embryonic development at 6 different temperatures (17.5˚C, 20˚C, 22.5˚C, 25˚C, 27.5˚C, and 30˚C). Every second of video is 15 minutes of development.
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Title D. virilis at 6 temperatures
Downloaded 17 times
Description D. virilis development at 6 different temperatures (17.5˚C, 20˚C, 22.5˚C, 25˚C, 27.5˚C, and 30˚C). Scaling is 15 minutes of development per second of video.
Download Dvir_allTemps_25.avi (300.3 Mb)
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Title D. pseudoobscura at 6 temperatures
Downloaded 13 times
Description D. pseudoobscura embryonic development at 6 different temperatures (17.5˚C, 20˚C, 22.5˚C, 25˚C, 27.5˚C, and 30˚C). Every second of video is 15 minutes of development.
Download Dpse_allTemps_25.avi (212.8 Mb)
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