Sewage loading and microbial risk in urban waters of the Great Lakes
McLellan, Sandra; Sauer, Elizabeth; Bootsman, Melinda (2018), Sewage loading and microbial risk in urban waters of the Great Lakes, DataONE, Dataset, https://doi.org/10.15146/R3S962
Theis dataset contains untreated sewage (wastewater treatment plant influent) samples from Milwaukee, WI, USA collected over a two year peroid. These were analyzed by qPCR for human associated indicators human Bacteroides (HB assay) and human Lachnospiraceae (Lachno2 assay), and by qPCR for E. coli and enterococci. Water samples were also collected at the estuary using a Telodyne ISCO sampler to perform automated, high frequnency sampling of 15 minute subsamples. Hourly samples were analyzed by culture for E. coli and enterococci. Composites of 1, 2, or 4 hours were analyzed by qPCR for These samples were analyzed by qCRP for HB, Lachno2, E. coli, and enterococci.
BLD is below the limit of detection
BLQ is below the limit of quantification, whihc is 225 CN/100 ml for all qPCR assays
National Institutes of Health, Award: R01AI091829
Milwaukee Metropolitan Sewerage District, Award: M03029P07