Mutations in a novel cadherin gene associated with Bt resistance in Helicoverpa zea
Data files
Aug 28, 2020 version files 5.84 GB
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cad86c_qpcr.xlsx
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Hzea_cad86c_Primer_Barcodes.xlsx
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m54163_180608_183238.subreads.bam
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myHzCAD86c1bALL.fasta
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myHzCAD86c2bALL.fasta
Abstract
Methods
There are three data types associated with this data set.
The first is raw Pacific Biosciences (PacBio) long read sequences produced from PCR amplified cad-86C cDNAs (m54163_180608_183238.subreads.bam). We have also provided the barcodes that can be used to demultiplex these PacBio data using the demulti_bbduk.sh script provided at https://github.com/rguo1990/Hzea_PacBio_sequencing.
The second is a pair of fasta files containing amplicon sequences produced by cad-86C primer pairs 1b and 2b.
The third is an excel spreadsheet containing the raw qPCR data used to test whether cad-86C was expressed in the H. zea larval midgut.
Usage notes
In order to demultiplex the PacBio dataset, please use the barcodes provided in the barcode file along with the demulti_bbduk.sh script provided at https://github.com/rguo1990/Hzea_PacBio_sequencing.